Dissertation > Excellent graduate degree dissertation topics show

Study on common probiotics detection and identification using MALDI-TOF MS Technology

Author: FanTieNan
Tutor: ZouJiHong; YuanJieLi
School: Heilongjiang University
Course: Microbiology
Keywords: matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) conventional phenotypic identification 16S rDNA sequence homology analysis strain identification probiotics
CLC: Q93
Type: Master's thesis
Year: 2011
Downloads: 67
Quote: 0
Read: Download Dissertation

Abstract


The screening and identifying methods of the candidate probiotic strains, as an important research part of probiotic industry, has been paid great concerns by researchers. In recent years, Matrix-assisted laser desorption ionization time of flight mass spectrometry, which was applied to screen and identify strains based on protein fingerprints of strains, is widely researched because of its characteristics of accuracy, rapidity and high-throughput.In this research, the optimal protocol of detecting and identifying common probiotic strains was firstly established by investigated the influencing factors of identification process, which includes different analytic methods, different matrix substances, different matrix solvents, different solvents of treating sample, different spotting methods and ratios,etc. It also proved that the identification results of Matrix-assisted laser desorption ionization time of flight mass spectrometry possessed highly stability and excellent repeatability. When colonies exceed 104 cfu, experimental strains can be identified to species level by Matrix-assisted laser desorption ionization time of flight mass spectrometry.In addition, to investigate the applicability of Matrix-assisted laser desorption ionization time of flight mass spectrometry, conventional phenotypic identification methods and 16S rDNA sequence homology analysis were used as the control methods. The results demonstrate that Matrix-assisted laser desorption ionization time of flight mass spectrometry was accurate, rapid, simply, inexpensive and can high-throughput identify strains to the genus, species, subspecies and even strain level. Finally, good application prospect of the method was showed in probiotic industry.

Related Dissertations

  1. Study on the Combined Probiotics and Its Effects on Prodution Performance of Pigs,S828.5
  2. Effect of Probiotics on Growth Performance, Digestive Enzymes Activities, Immunity and Gut Microbiota of Epiniphelus Coioides,S917.4
  3. The Research of Chinese Medical Herbs and Probiotic on Controlling Diarrhea and Weight Gain in Piglet,S828.5
  4. Effects of Solid Ferment Technological Parameter on Ferment Quality of Rapeseed Meal,S828.5
  5. Selection and Biological Assessment of the Probiotic Microorganisms in Hog-feeding,S828.5
  6. Probiotics on growing pigs and fecal microbial indicators of impact,S828.5
  7. Different combinations of probiotics on growth performance and anti- diarrheal effects of,S828.5
  8. Effects of Probiotics and Achyranthes Bidentata Polysaccharides on Growth Performance and Meat Quality of Pigs,S828.5
  9. The Physiological Response of Lactobacillus Casei ATCC 393 under Different Stresses,TS252.1
  10. Screening and Characterization of Probiotic Lactobacillus Strains with Inhibitory Activity to Monoamine Oxidase,TQ460.1
  11. Studies on Microflora of Yellowy Catfish (Pelteobagrus Fulvidraco) Intestine and Probiotics,S917.1
  12. The Screen and Evaluation of the Probiotics of Resistance to Shrimp White Spot Syndrome Virus,S945
  13. Research and Development of a Novel Microbial Ecological Agent,S816.7
  14. Study on the Production of Viable Cells Feed by Lactic Acid Bacteria with Fermentation of Corn Starch Cake,S816
  15. Study on Effects of Probiotics on Production Performance and Immune Function of Weaning Piglets,S828.5
  16. An Experimental Study of Compound Probiotics with Lactobacillus Acidophilus, Lactobacillus Rhamnosus and Bacillus Subtilis in Animal Models of Cow Endometritis,S858.23
  17. Study on the Removal and Mechanism of Microcystin-LR by Probiotics,X172
  18. With angiotensin-converting enzyme inhibitory activity of probiotic screening,Q936
  19. Studies on Anguilla Anguilla Intestine Probiotics for the Prevention and Cure of the Disease,S94
  20. The Effects of Probiotics on Performance and Intestinal Microflora of Growing Pigs,S828.5
  21. Industrial Producing Condition of Selenium-Enriched Probiotics and Its Inhibitory Activity Against Pathogenic Escherichia Coli,S816.72

CLC: > Biological Sciences > Microbiology
© 2012 www.DissertationTopic.Net  Mobile