Dissertation > Excellent graduate degree dissertation topics show

The Optimization of Fermentation Conditions of Sacs-Producers and Purification of BS Produced by Bacillus Sp. SAC-11

Author: FangYuZuo
Tutor: ZhangYuZhen
School: Shandong University
Course: Microbiology
Keywords: Surface active compounds lipopeptide production chromatography purification
CLC: TQ423
Type: Master's thesis
Year: 2012
Downloads: 182
Quote: 0
Read: Download Dissertation

Abstract


Biosurfactant is a new type of surfactant which developed in recent years. Besides reducing surface tension as synthesized surfactants, Biosurfactant has superior properties, such as low toxicity, readily biodegradable, good ecological compatibility features etc. With the rapid development of biotechnology and related techniques, Biosurfactants not only in traditional industries will gradually replace chemical surfactants and may exceed the scope of application of traditional surfactants, playing a significant role in pharmacological effects of antibacterial, antiviral, antitumor, and immune function.In this study, a wild biosurfactant producing strain SAC-11was mutated by UV irradiation to obtain the strain SAC-2-02which increasing the surfactant production by38.43%than the original strain under the same conditions. After a series of optimization work of fermentation conditions, we determined the best carbon and nitrogen sources for bacterial fermentation of glucose and inorganic ammonium salt, C/N ratio is20/3, Carbon source concentration is60g/L. Under these conditions, the96h fermentation broth lipopeptide production is1.55g/L. Effects on biosurfactant production of other culture conditions, in addition to medium composition, such as ventilation, incubation temperature, initial pH, inoculum size, and ionic strength were studied by orthogonal experiments, the best theoretical combination of conditions were obtained:200rpm,28℃, initial pH7.0, inoculum size3%and sodium chloride3%. Under the former conditions, the biosurfactant production can reach1.87g/L,2.8times higher than SAC-11in ordinary mineral salts medium. The production is generally higher than generally reported in the lipopeptide surfactant productions.We also extracted the lipopeptide surfactant produced by strain SAC-11by acid precipitation, organic extraction and double column chromatography purification. Full-band UV scanning results of the collection component showed that the surfactant samples have no UV absorption properties. Samples eluted by HPLC with ELSD detector showing four consecutive elution peak. Changing the mobile phase liquids or elution conditions was still difficult to completely separate several peaks. The further purification of the sample may require a separation by semi-preparative high pressure liquid chromatograph.

Related Dissertations

  1. Study on Protective Effects of Apple Polyphenol on Immune System Injury Caused by γ-Ray,S661.1
  2. Study on Wastewater Purification Effect of the Free Surface Wetland with Different Substrates and Plant Combination,X703
  3. Penicillium Expansum TS414 Lipase in Pichia Pastori: Expression, Purification and Enantioselective Esterification of (R, S)-naproxen,Q814
  4. Studies on Fermentation Optimization, Purification and Enzyme Characteristics of Lipase from Aspergillus Oryzae FS-1,TQ925.6
  5. Studies on Isolation, Purification and Chemical Structure for Polysaccharides from Camellia Chrysantha(Hu) Tuyama,S567.19
  6. Discovery and Biological Activities of Natural Michael Addition Acceptors,R284
  7. Research of Glycosaminoglycan from Perna Virids Effect on Blood Lipid,R285.5
  8. Purification of Endogenous Proteases from (Litopenaeus Vannamei) Shrimp Head and Their Enzymology Properties,S985.21
  9. Study on the Preparation of the Low Molecular Weight Fuciodan from Sargassum Henslowianum (C.Agardh) and Anti-tumor Activity,TS254.9
  10. Synthesis and Resolution Property of N-(9-Fluorenylmethoxycarbonyl)-D-Tryptophan and D-Phenylalanine Imprinted Polymer,O631.3
  11. Under microaerobic conditions closed calcium carbide furnace exhaust COS gas adsorption purification,X781
  12. The Study on Rhinoceros Horn and Its Imitation,TS932.2
  13. Studies on Extraction and Analysis of Fructooligosaccharides in Smallanthus Sonchifolius,TS255.1
  14. Research on Melamineanalysis Method in Milk Powder and Establish the System of Rapid Determination,O657.3
  15. Fast Determination Method Research of Bisphenol A in Food Packing Material,TS206.4
  16. The Research on Sulfate Leaching Properties of Cation-exchange Resin Used in Power Plants,TQ425.23
  17. Experiment Study of the Biogas Purification Process by Zeolite Filling Washing,TQ221.11
  18. Optimization of Fermentation Conditions, Purification, Cloning and Expression of a Cold-active Lipase from Pseudomonas Sp.RT-1,TQ925
  19. Extraction, Purification and Antifungal Activity of Anthocyanins in Drop Fruits of Chinese Baberry,TQ914.1
  20. Preliminary Research on Characterization of Thermophilic Protease Pasey and Helicase Htc16,Q814
  21. Study on the Separation and Purification Way of Arctiin from Fructus Arctii and the Effect of Arctiin on the Growth of Mice C2C12 Cells,S853.7

CLC: > Industrial Technology > Chemical Industry > Reagents and the production of pure chemicals > Surfactant
© 2012 www.DissertationTopic.Net  Mobile