Dissertation > Excellent graduate degree dissertation topics show

Extraction and Biological Activities Studying of Phospholipids from the Skipjack Brain

Author: ZhangLi
Tutor: HuJianEn
School:
Course: Aquatic Products Processing and Storage Engineering
Keywords: The Skipjack brain phospholipids ultrasonic wave assisted extraction antioxidative immuno-regulation
CLC: TS254.5
Type: Master's thesis
Year: 2013
Downloads: 30
Quote: 0
Read: Download Dissertation

Abstract


The phospholipids are important biologically active substances, have mainfunction in nutrition and health.The phospholipids can prevent and treat highcholesterol and hyperlipidemia, also play an important role in anti-oxidation,anti-aging and so on. Phospholipids containing a hydrophilic group and hydrophobicfatty acid radicals, are a typical amphoteric compound,which have good emulsifyingproperties. Currently, have been widely used in terms of food, medicine, health careproducts.In this study, the extraction technology of phospholipids from the Skipjackbrain was studied by ultrasonic method,and the composition and activity of thephospholipids were studied, the study results as follows:1、Extraction factors of ultrasonic method were optimized by single factorexperiment and orthogonal experiment. The optimal conditions were as follows: theratio of hexane and ethanol is2:1,40min,35℃,1:9of the ratio of material to solvent,ultrasonic power300W,ultrasonicextraction2times, respectively. Under theseconditions, the content of phospholipids from Skipjack brain was19.59mg/g.2、 It contains phosphatidylethanolamine(PE), phosphatidylcholine (PC),sphingomyelin (SM) according to the standards by the thin-layer chromatography(TLC).Phospholipids fatty acids composition of skipjack brain was determined by gaschromatography,which contains high unsaturated fatty acid, such as21.1%DHA and2.4%EPA.3、 The anti-oxidative activity of phospholipids from the Skipjack brain wasstudied.The results showed when the concentration of phospholipids from theSkipjack brain at1000μg/mL that the reducing power is highest, DPPH scavengingradical activity and hydroxyl radical scavenging activity were23.7%and43.3%.Inaddition,anti-oxidation activity of phospholipids from the Skipjack brain on edibleoils was studied using by Schaal oven-storage test.The results showed that thephospholipids from the Skipjack brain are of good anti-oxidant effects on soybean oiland olive oil.When the phospholipids in oils was0.5%,the anti-oxidative effect was asgood as0.02%TBHQ.4、 The phospholipids from the Skipjack brain emulsifying capacity studies haveshown that under the same conditions the phospholipids from the Skipjack brain havea better emulsifying capacity than the soybean phospholipids.5、 The immunomodulating effect of the phospholipids from the Skipjack brainwas studied on the RAW264.7macrophages.The macrophages were stimulated by the phospholipids from the Skipjack and became activated cells.The volume,cellviability and phagocytosis of the cells were increased.The concentration of NO andthe activity of SOD inside the cells were also enhanced.6、 The gene expressions of IL-1α、IL-6、IL-8、IL-10、IL-12、TNF-α mRNAwere assayed by RT-PCR.It suggested that the immunomodulating effect ofphospholipids may be associated with cytokine mRNA expression.

Related Dissertations

  1. The Antioxidative Efficient Component Recognition for Curcuma Longa L. Based on the Quality Control Pattern of Constituent Knock-out/Knock-in,R285
  2. Cadmium, Chromium and Lead Accumlation, Distribution in Aralia Elata Var. Inermia and Their Effect on Leaf Antioxidative System,S792
  3. Study on Preparation and Antioxidative Activity of Tea Residues Polypeptide,TS209
  4. Research Physiological Response and Antioxidative Mechanism of Wheat (Triticum Aestivum L.) Seedings under Copper or Cadmium Stress,S512.1
  5. The Pharmacological Action of Fritillaria Unibracteata Hsiao Et K.C. Hsia,S567.231
  6. The Application of Metabolite Target Analysis and RT-PCR in Disease Research,R541.4
  7. Study on Preparation and Stability of Allicin Lipsomes,TS255.1
  8. Research of the Effect of Dietary High-cholesterol Seafood on Lipid Metabolism in Animals,TS254.1
  9. A Study on Production of Antioxidative Whey Peptides in Tangential Flow Filter Enzymatic Membrane Reactor,TS201.2
  10. Study on Preparation Oface Inhibitory Peptides and Antioxidative Peptides from Oat Protein by Enzymatic Membrane Reactor,TQ464.7
  11. Screening of Antioxidative Lactic Acid Bacteria and Its Application in Aquatic Product Processing and Preservation,S983
  12. AFLP Screening of the Differential Probiotic Genes of Lactic Acid Bacteria,R371
  13. Study on Antioxidative Peptide of Peanut Meal,TS229
  14. The Study on the Prevention and Therapy of the Extractive of Cordyceps on Anti-thymocyte Serum Nephritis Model,R285.5
  15. The Immunoregulation Effects of Bone Marrow Mesenchymal Stem Cells on the Lymphocytes of Rats with Type 1 Diabetes Mellitus in Vitro and in Vivo,R587.1
  16. Effects of Dietary Valine Digestive Ability, Immune Function and Antioxidative Ability of Juvenile Jian Carp (Cyprinus Carpio Var.Jian),S965.116
  17. The Study the Immune Regulation Effect of Haptoglobin of Premature Coronaryartery Disease,R541.4
  18. Study on Antioxidant Activity and Phenolic Composition Analysis of Coconut Inflorescence Sap,R284
  19. Preparation of Active Peptides of Silkwormpupa Protein by Enzymatic Hydrolysis,S886.9
  20. Studies on Immunological Characteristics of the WH Rabbit Erythrocytes at Different Development Stages,S852.4
  21. Immune Effect of Thymosin-α1 on CD4 ~+ CD25~ + Regulatory T Cell in Septic Mice,R459.7

CLC: > Industrial Technology > Light industry,handicrafts > Food Industry > Aquatic products processing industry > Aquatic products
© 2012 www.DissertationTopic.Net  Mobile