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Antiviral protein as a potential new class of antiviral drugs has been a research focus at home and abroad, scholars have reported housefly substances containing antiviral activity in vivo. Housefly (Musca domestica L.), belonging to the Insecta (Insecta), Diptera (Diptera), ring crack suborder (Cyclorrhapha), Muscidae (Muscidae). The housefly larvae material extracted maggot protein detection anti-viral and anti-tumor activity, the main findings are as follows: 1. Soxhlet extraction from housefly larvae a lipoprotein, pseudorabies virus (Pseudorabies Virus, PRV) as a target virus, chicken embryo fibroblasts (CEF), viral vectors, and studied Housefly crude extract apolipoprotein antiviral activity. The findings of PRV virus with crude protein (0.1mg/m1) After mixing for 2 hours at room temperature, the titer of from 5.62 × 104 (TCID50 M1-1) decreased to 3.16 × 104, crude protein against PRV virus The presence of inhibitory activity; 1 hour prior to infection and one hour after infection the virus titer of the protein measured Add crude extract were 3.16 × 104 and 1.0 × 104, indicating that the crude protein may be provided to the virus adsorption injection phase sensitive. 2. Crude protein was studied against PRV virus on CEF cells adsorption effect of the injection phase: crude protein was added prior to infection of the virus 1 and 2 hours, the virus infected cell culture bottles supernatant collected after 2 hours, the measured The virus titers were 5.62 × 104 and 2.51 × 104, still contains large amounts of virus in the supernatant, showed that the crude protein was added before viral infection, can affect the PRV the adsorption of the virus on CEF cells injected. Using ion exchange chromatography purified housefly larvae recovered two peaks in the crude protein, A1 and A2. Determination of the antiviral activity of the respective components, respectively, A2 purified protein (0.2mg/m1) after with the mixing action of virus, the virus titer of from 5.62 × 104 to 3.75 × 104, A1 purified protein on the virus titer is not impact. 4. Using the MTT assay, respectively, A1 and A2 purified protein inhibition studies of anti-tumor activity of A549 cells, results showed that the A1 purified protein has no effect on A549 cells, and A2 purified protein was significantly inhibited A549 cells, and with the significantly A2 protein concentration was increased, the inhibition rate rise, indicating that the purified protein A2 is the desired target protein. The experimental the the housefly larvae antiviral protein anti-PRV virus and anti-A549 cell research, anti-viral activity of protein for insects to provide an experimental basis.
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