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Isolation and Characteristics of Efficient Carbendazim Degradation Bacterium

Author: LinXiu
Tutor: HouZhenAn
School: Shihezi University
Course: Plant Nutrition
Keywords: Carbendazim Biolog Isolation and identification of Soil degradation
CLC: X172
Type: Master's thesis
Year: 2011
Downloads: 41
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Abstract


Objective: Xinjiang arid farmland soil screening carbendazim degrading microorganisms efficiently, and to explore its degradation characteristics, provide a theoretical basis for the arid zone control and remediation of soil contaminated by pesticides. : Broad-spectrum fungicides - carbendazim as the research object, through the indoor simulation experiments, separated from the long-term application of carbendazim vineyards screening of microorganisms capable of degrading carbendazim by Biolog Microbial Identification System 16S rDNA sequences, as well as the system cluster analysis identified strains. Residues of carbendazim was determined by high performance liquid chromatography, and then study the degradation characteristics of carbendazim degrading bacteria. Results: 1, in the long-term application of carbendazim vineyard soil, initial separation, screening 16 strains carbendazim as the sole carbon and nitrogen source growth after carbendazim degradation of the ability to detect degradation the two bacteria degradation strongest, named XJ-D and XJ-H, and 15 d after carbendazim degradation rate of the two strains of 99% and 97.3%, respectively, belong to carbendazim efficient degrading strains. 2 pair and hierarchical cluster analysis of the strains were identified by Biolog Microbial Identification System and 16S rDNA sequences than. Identification results: strain XJ-D Rhodococcus (Rhodococcus erythropolis), strain XJ-H Azospirillum brasilense Azospirillum brasilense. 3 in the medium environment, research carbendazim degradation strains XJ-D and the degradation efficiency of the XJ-H. The carbendazim concentration of 600 mg / L degradation medium strain XJ-D and XJ-H in the 11d degradation rate of carbendazim 99% and 95.6%, the average degradation capacity to 52.87 mg / (L · d)] 52.24 mg / (L · d), when the strains of the number in the 3d has reached the maximum carbendazim degradation rate of 87.24% and 78.1%. (1000 mg / L) in the high concentration of carbendazim degradation liquid, strain XJ-D degradation rate of 27.68%, the average degradation efficiency of 24.83 mg / (L · d), the degradation rate of strain XJ-H 60.4%, with an average degradation efficiency of 54.08 mg / (L · d), although the high concentration of carbendazim inhibited two strains, but strain XJ-H showed better adaptability. In carbendazim degrading strains sterilized soil degradation simulation test, the strain XJ-D showed good degradation characteristics, carbendazim degradation rate is significantly higher than those not inoculated control after vaccination degrading strains XJ-D . In the soil carbendazim concentration of 200 mg / kg, 15 days carbendazim degradation rate of 82.81%, the average degradation efficiency of up to 11.04 mg / (kg · d), the half-life of 6.28d control treatment carbendazim degradation rate of 25.27%, the average degradation efficiency of 3.37 mg / (kg · d), the half-life of 34.58d. Simulation test proved by the soil, the bacteria XJ-D significantly promote the degradation of carbendazim in soil. Strain XJ-H, although from carbendazim contaminated soil, but when inoculated into the soil, may be due to environmental factors in the test soil microenvironment limit strains XJ-H did not survive in the soil of . 5, in the indoor simulation of carbendazim degradation strain soil degradation degradation conditions of the test, get the carbendazim efficient degradation the strains suitable soil XJ-D carbendazim concentration of 200 mg / kg; most suitable inoculum size 109 / g soil; The appropriate temperature for 23 to 28 ° C; soil moisture range of 60% to 80%.

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