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The Biological Effect of 35 GHz Millimeter Wave and Simple Heat Environment Radiate on Mice

Author: LiZhiHui
Tutor: LiuHuZuo
School: Northwest University of Science and Technology
Course: Biochemistry and Molecular Biology
Keywords: Millimeter wave Thermal radiation Survival time RT-PCR hsp70
CLC: Q691
Type: Master's thesis
Year: 2010
Downloads: 6
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Abstract

The millimeter wave is the wavelength of 1 ~ 10 mm, and the frequency of the electromagnetic wave within the range of 30-300 GHz. The millimeter-wave focused at 35 GHz, 60 GHz and 94 GHz the few millimeter wave frequency window, used for rehabilitation medicine (physical therapy) and in the military field. The main characteristics of the millimeter wave is small penetration capability, in distilled water, the penetration depth is only 0.78 ~ 0.23 mm, and a short time when irradiating human and animal, only through the tissue surface shallow reach the dermal papillary layer and network like layer, resulting in damage to the cornea, skin, millimeter wave and vulnerable to the impact of different media in the dissemination process. However, high power millimeter wave within a short period of time (a few seconds) but the skin can produce a burning like tingling, and a turn off the millimeter-wave transmitter or leave millimeter wave irradiation area, this feeling will disappear therefore millimeter wave fast aversion effect to achieve the effect without causing casualties case can quickly disperse the crowd. Therefore, this study millimeter wave preliminary millimeter wave radiation lethal experiments with mice and sustained acute thermal radiation lethal murine experimental study aimed at obtaining high power millimeter wave irradiation lethality in mice of different combinations of threshold parameters (power density lethal irradiation distance and irradiation time parameters), the variation between sustained acute thermal radiation time and the survival time of mice lethal radiation temperature and the corresponding pathological and morphological changes in preliminary elaborated millimeter and pure thermal radiation lethal effect on mice, and provide a reference for the follow-depth study of millimeter wave aversion effect. In order to study the characteristics of the 34.1 GHz high power millimeter wave radiation lethal mouse threshold parameters and histopathological and morphological changes, the mice in a normal posture (back of hypogastric) bound to a wooden plate placed in the operating frequency of 34.1 GHz The average output power of 5 W, 10 W and 12 W high power millimeter wave source vertically below the irradiation distance is 10 mm and 20 mm respectively, continued irradiated mice until death. Anatomy immediately take the skin, lung and brain tissue, quickly rinsed with 0.9% saline, placed in a 10% formaldehyde solution fixed, soaked with paraffin embedding and preparation of tissue sections. HE staining, CCD camera, using the IPP image analysis software, integrated average optical density and area than the the microscopic image quantitative analysis of pathological characteristics of the lungs. The results show that irradiation of HD-320-HA23.16 and HD-320-HA9.92 Model pyramidal horn antenna, the higher average power (12 W) continuous irradiation fastest can cause death within animal 110 sec; while lower The average power (5 W) continuous irradiation can lead to the death of the animals within 30 min. After irradiation the dead animals were seen in lung the obvious bleeding; different parameter combinations millimeter wave irradiation conditions, the degree of pulmonary hemorrhage. Wherein, when the average power of 10 W and 12 W, the degree of bleeding of the lungs in mice is the most serious, and pulmonary bronchi and blood vessels have obvious rupture phenomenon, bronchial also visible in the macrophages. When the average power of 5 W, the degree of pulmonary bleeding is significantly reduced, and no severe bronchial and vascular rupture phenomenon. Thus, the high-power millimeter-wave acute irradiation can lead to bleeding and damage in mouse lungs, lung tissue injury and millimeter wave radiation was significantly positively related to average power greater the more serious lung injury in mice. Because this study used high-power millimeter strong thermal effects, suggesting that acute, rapid thermal effects induced lung injury in mice may be an important cause of animal death. In order to further study the biological effects of the millimeter wave, we use the oven radiation simulation of the thermal effects of the millimeter wave. First, set a different radiation temperature radiation Balb / c mice, until their death. Study the animals were placed in the oven, set ambient temperature of 43 ~ 61 ° C (2 ° C increments) for acute sustained heat radiation, until the mice died. Timely recording of body weight before and after the time of death of the mice and the mice died. Dissected, immediately after the first removal of the small part of the hair of the back of mice skin and brain tissue fast rinsed with 0.9% saline, placed in 10% neutral formalin fixed and soaked in paraffin embedded and preparing tissue sections . HE staining, CCD camera, using the IPP image analysis software to analyze the degree of skin tissue damage and cerebral cortex neurons injury. The results show that presents a very significant exponential relationship between the survival time of mice with the radiation temperature, the R value can be achieved 0.9994; When the preset radiation temperature is not the same before and after radiation, the body weight of mice also have differences; but very significant difference compared with the control group, the mice showed a body weight before and after the irradiation; thermal radiation can also be changed so that the mouse skin structures or damaged, but also make the cerebral cortex neurons pyknosis. In short, the animals survived very significant correlation between the level of the length of time of acute heat radiation temperature. Furthermore, the sustained acute thermal radiation is not only an impact on the animal's weight, and also has a considerable influence on different tissues. Further, in order to verify that the thermal radiation from the molecular level in mice, the first most after removal of the back skin of mice after hair rapid rinsing in 0.1% DEPC water, stored in liquid nitrogen. Extraction of total mRNA and reverse transcription, PCR amplification and heat stress-related genes and gene expression intensity analysis found that the etc. of hsp70, krt6α and thbs1 gene expression results showed a clear upward trend in the different radiation temperatures. Which hsp70 expression is dramatically raised the ambient temperature is below 55 ° C, the upregulation of more than 57 ℃ relatively slow; the upregulation krt6α and thbs1 extent has nothing to do with the temperature changes. Furthermore, hsp105 the expression at a lower temperature (51 ℃), presented as the temperature rises upward trend; while at higher temperatures (above 53 ° C), but no significant differences. Shows different intensity acute thermal radiation significantly affect gene expression in mouse skin heat stress, and the correlation between different gene expression patterns and radiation temperature varies. The same time, according to the twofold PCR analysis results visible, hsp70 can be used as molecular markers to quantify the different intensity of thermal radiation injury, and has laid a good foundation for the identification and quantification of different combinations of parameters millimeter wave radiation damage in mice experiments from the molecular level. In summary, this paper is the use of millimeter wave and oven heat radiation are two ways to mice lethal effect of the lethal combination of different parameters (horn antenna, output power and irradiation distance) millimeter wave mice analysis, preliminary to obtain the lethal threshold parameter mice, in particular, to obtain the characteristics of millimeter wave on the lethal mouse lung damage; oven analog millimeter wave irradiation experiments obtained under different radiation temperature the mouse lethal time and the organizations characteristics of pathological changes, it is more important to get the radiation temperature and survival time between the law and interrelated, and laid the foundation for the subsequent depth study of the biological effects of the millimeter wave irradiation.

The Biological Effect of 35 GHz Millimeter Wave and Simple Heat Environment Radiate on Mice

Abstract

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