Dissertation > Excellent graduate degree dissertation topics show

The Influence on Oxidation and Anti-Oxidation of Chickens Fed with Probiotics after Vaccinated by Infectious Bursal Disease Vaccine

Author: JingLong
Tutor: ZhengShiMin
School: Northeast Agricultural University
Course: Basic Veterinary Science
Keywords: probiotic chickens IBD oxidation anti-oxidation
CLC: S858.31
Type: Master's thesis
Year: 2012
Downloads: 66
Quote: 2
Read: Download Dissertation

Abstract


To reveal the influence of probiotics on oxidation and anti-oxidation of chickens which were vaccinated by IBD vaccine, challenged with virulent IBDV, the methods of histochemistry staining and immunohistochemistry were used to detect the changes of T-SOD, MDA and NO content in serum and some organs, such as heart, liver, spleen, kidney and bursal, and the changes of histopathology were also observed in order to reveal the damage of oxidation. The different types of SOD in organs were detected by PCR. This experiments could provide the scientifitic evidence for the development of probiotics. The results were as follows:1. After one-day-old chickens fed with probiotics, the content of T-SOD in serum and organs was much stronger than that of controls (P<0.05or P<0.01), after challenged with virulent IBDV, the content of T-SOD in serum and organs was much stronger than that of controls and chickens which were just vaccinated by IBD vaccine without probiotics fed (P<0.01). These results indicated that the anti-oxidation enzyme was expressed stronger in chickens fed with probiotics after challenged with virulent IBDV, and, the function of anti-oxidation was inhanced remarkably.2. The content of MDA in serum and organs was much more lower than that of controls and chickens which were just vaccinated by IBD vaccine without probiotics fed (P<0.05or P<0.01). By observing the changes of histopathology, the damage of probiotics fed chickens organs was much more lighter than that in controls and chickens which were just vaccinated by IBD vaccine without probiotics fed. The bursal has the fiber hyperplasia, including reticular fiber, collagenous fiber and elastic fiber which was the least fiber content. These results indicated that the probiotics fed chickens could be better protected from damaging after challenged with virulent IBDV than that in controls and chickens which were just vaccinated by IBD vaccine without probiotics.3. The content of NO in serum and organs including the content of iNOS in organs was lower than that of controls (P<0.01), neverthelessly, it was higher than that in chickens which were just vaccinated by IBD vaccine without probiotics fed (P<0.05or P<0.01). The results indicated that probiotics could stimulate the macrophage proliferation which could result in upregulating the content of iNOS in organs. NO has the anti-virus activity and cytotoxic activity, the latter was more obvious in virulent IBDV infected chickens.4. Probiotics Ⅰ group chickens with Lactobacillus primarily, the protection of anti-oxidation was better than probiotics Ⅱ group chickens with Bacillus primarily.

Related Dissertations

  1. Preparation of Micro-Arc Oxidation Ceramic Coating on Mg Alloy and the Research of Galvanic Corrosion,TG174.453
  2. Study on the Degradation of 2-MIB in Aqueous Solution by ZnOOH Catalized Ozonation,X703
  3. Studies on Extraction, Isolation, Purification and Antioxidation Activity of Flavonoids from the Fruits of Avicennia Marina,S793.9
  4. The Study of NO Low-temperature Catalytic Oxidation Over MnFeO_x Catalyst,X701
  5. The Study on Anti Tumor and Anti-oxidation Activities in Vitro of Extracts from Oxalis Corniculata L,R285
  6. The Impact of Atorvastatin- reduced Glutathione Combination to Anti-oxidation Action and Blood Lipid Level in Rats with Hyperlipidemia,R965
  7. Effects of N Addition on Soil Soluble Nitrogen and CH4 Oxidation of Upland in Subtropical,S714
  8. Effects of Several Immunostimulants on the Growth Performance, Non-specific Immunity and Disease Resistance of Cobia, (Rachycentron Canadum),S963
  9. Solid Oxide Fuel Cell ferrite connect the material properties of,TM911.4
  10. Research on Biological Modification and Protection Activity of R-Phycoerythin from Porphyra Yezoensis,TS201.21
  11. Study on Extraction,purification and Antioxidant Activity of Wheat Germ Polysaccharides,TS210.1
  12. Effect of Pyruvate, Creatine and Creatine-Pyuvate on Profile of Amino-Acids and Relevant Enzyme Activity in Broiler Chickens,S831.5
  13. Invasion of Human Shigella to Chicken and Intestinal Epithelial Cell and the Expression of CD44 in Developing Chicken Intestine,S855.1
  14. The Dynamic Distribution of Shigella Isolated from Chicken in Infected Chickens,S858.31
  15. Effects of Creatine Pyruvate on Lipid and Protein Metabolism in Broiler Chickens,S831.5
  16. The Anti-oxidation Activity and Microencapsulation of the Extract from Eucommia Ulmoide Oliver Leaves,R285.5
  17. The Research of Fast Integrated Physicochemical Technology for Emergent Treatment of Organic Contamination Accidents,X703
  18. Effects of in Ovo Equol Injection on Posthatch Growth and Meat Quality of Broilers and Mechanism Research,S831
  19. Treatment of Tannery Wastewater by Combined Process of Two-stage H/O and Ecological Pond: Study and Practice,X794
  20. Study on the Effect of Natural Antioxidant and Edible Film for Dry-Cured Meat Products,TS251.6
  21. Equipment Research of the Ship Sewage Treatment,X703

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Livestock, poultry, wildlife diseases > Poultry > Chicken
© 2012 www.DissertationTopic.Net  Mobile