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Effects on GAS in Gastrointestinal Tissue and Serum of Rats with Functional Constipation Model Treated by Electroacupuncture

Author: YueFengJie
Tutor: LiuZhiBin
School: Shaanxi College of Traditional Chinese Medicine
Course: Acupuncture and Massage
Keywords: Electroacupuncture Functional constipation GAS
CLC: R246
Type: Master's thesis
Year: 2013
Downloads: 54
Quote: 0
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Abstract


Objective: to use certain parameters in the model rats ofelectroacupuncture constipation different acupoints combination, analysissummed up the rat gastric secrete element in serum and gastric tissue (GAS)content changes, and explore the biological regulation mechanism ofacupuncture treatment of functional constipation; And through comparing,curative effect to optimize acupoints combination, and to provide referencefor optimization of acupuncture and moxibustion treatment of functionalconstipation and basis.Methods:60rats were randomly divided into six groups, each group of10,only single only single cage close up, LongXia pad filter paper. Divided intoblank control group, normal temperature water lavage group (22~26℃0.9%NaCI solution) group, model control group, ice water to fill the stomach Ⅰfind group (units), ice water to fill the stomach Ⅱ group (yuan find group),ice water to fill the stomach Ⅲ group (unit+sets yuan find group). Don’tdo any deal with blank control group, normal temperature water lavage groupwas given3ml/22~26℃0.9%NaCI solution only to fill the stomach, modelcontrol group and the ice water to fill the stomach Ⅰ, Ⅱ, Ⅲ group was given0-4℃3ml/only to fill the stomach. Once a day for building14days. Toeliminate the influence of the biological rhythm, at the same time every dayto bondage stress. Stop after lavage group continued to normal feeding food,drink, watch14d, until the end of28d mold maker. Pivot unit find groups:day, e. shu (bilateral); Set yuan find groups: li11, the giant virtual(bilateral); Pivot unit+sets yuan find groups: day, e. yu, li11, the giantvirtual (one sided). Adopt Korean electric acupuncture apparatus (LH202H type) treatment, select the density wave, the frequency of thin wave2hz, densewave15hz, the current strength of1.5mA, in order to attract rats body slightjitter for degrees, retaining needle for20minutes, warm water to fill thestomach does not do the electric acupuncture treatment group and model controlgroup,1time/day,10times for a period of treatment, evaluation of curativeeffect after a period of treatment.The result: ice water to fill the stomach to reduce GAS content in theblood of the rat. Unit find and unit set yuan find group can make the GAS contentin blood of rats was increased, but not back to normal levels, set yuan findgroups can enable higher GAS content in the tissue of rats and close to normal;Ice water to fill the stomach can reduce GAS levels in the organization ofrats, cupping treatment three groups can make the GAS content in blood of ratswas increased, yuan find group can make gastric antrum of rats, higher GAScontent in the colon and close to normal.Conclusion: through this experiment shows that the electricity infunctional constipation has good treatment effect, but the curative effectof acupuncture point method is different, different group of serum and gastrictissue showed that the gastric secrete element changes in RMB set thetherapeutic effect of acupuncture point group best; Combined with relatedliterature and theory of neurology, preliminary discussed the mechanism ofacupuncture treating constipation and closely related to neural and humoralregulation, its core lies in the brain and spinal cord nerve effects ongastrointestinal tract.

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CLC: > Medicine, health > Chinese Medicine > Chinese medicine clinic > Acupuncture, acupuncture therapy > Clinical applications of acupuncture
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