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Objective use low sodium, potassium, calcium ions irrigation isolated frog heart, observe wenxintang and serum face three kinds of ions perfusion after isolated frog heart the influence of systolic function, Preliminary discuss the Wenxintang wrong rhythm of mechanism, And for clinical treatment rhythm of the heart wrong to provide the basis.Methods12mice randomly divided into two groups:the blank group and medicated serum serum group, preparation blood to spare.Take toad frog conventional preparation heart model random into three groups:including medicine blank serum serum group, and Wenxintang groups, and only10;Blank serum, including medicine serum, and Wenxintang to perfusion low sodium, potassium, calcium ions from isolated frog heart after myocardial contraction activities were compared. From isolated frog heart to heart rate and the myocardial frog tension as observation index, application RM6240way more signal acquisition and processing system detecting heart rate and average frog myocardial tension value of isolated frog heart.Results (1) a low sodium,low potassium ion solution perfusion from the body, when the heart heart rate a frog perfusion added fast before (P<0.01), low calcium ions perfusion solution from the body, when the frog is perfusion heart heart rate before a little slow (P<0.05); Low sodium, low potassium ions perfusion from the body, when the frog is perfusion heart myocardial tension before enhanced obviously (P<0.01), low calcium ions perfusion solution from the body, when the frog is perfusion heart myocardial tension before decreased significantly (P<0.01).(2) Blank group compared with serum, including medicine serum group and Wenxintang all to a low sodium irrigation flow from the body of the heart rate significantly slow heart frog (P<0.01), average myocardial tension decreased significantly (P <0.01); Medicated serum group and Wenxintang group are to make low potassiumperfusion from isolated frog heart after the heart rate significantly slow (P<0.01), average myocardial tension enhanced obviously (P<0.01); Medicated serum group and Wenxintang all to low calcium irrigation flow from isolated frog heart rate increased significantly faster (P<0.01), average myocardial tension enhanced obviously (P<0.01).Conclusion wenxintang and serum Improve low sodium, potassium, calcium induced isolated frog heart arrhythmia of the role, wenxintang and serum mechanism of action may participate in the activities of the cardiac arrest and sodium, potassium, calcium ion relevant.
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