Dissertation > Excellent graduate degree dissertation topics show

Experimental Study on Promoting Bone Consolidation by Platelet-rich Plasma in Different Period During Distraction Osteogenesis

Author: ShiBoWen
Tutor: ShuHengSheng
School: Tianjin Medical University
Course: Surgery
Keywords: Distraction osteogenesis Platelet-rich plasma Bone consolidation Rabbit
CLC: R687.3
Type: Master's thesis
Year: 2013
Downloads: 2
Quote: 0
Read: Download Dissertation


Objective To establish the rabbit model of tibial lengthening using the monolateral external fixation. Injecting platelet-rich plasma (PRP) at different period on distraction osteogenesis, To observe the effect on the distraction osteogenesis. To discuss the effect on the mineralization process of distraction osteogenesis at different period, and provide the theoretical basis on promoting bone consolidation and healing.Methods Forty Japanese rabbits (weighing2.5-2.8kg) were randomly divided into4groups (n=5). The experimental group was performed the tibial osteotomy fixed the monolateral external fixator and began distraction at five days after surgery in a rate of lmm/d for twice a day. In group A,5days after operation, inject PRP500ul1times in the beginning of lengthening; In group B,15days after operation, inject PRP500ul1times in the beginning of; In group C,25days after operation, inject PRP500ul1times at the middle of mineralization period; In the group D (control group), lengthening without injection of PRP. The X-ray films were taken at0,15,25,35days after operation, At37days after operation, the tibial specimens were harvested and HEcoloration and scanning electron microscope(SEM) was used to observe bone regeneration.Results1. X-ray:The X-ray films showed that new bone formation in groups B and C was obviously better than that in groups A and D at35days after operation.2. Measure the trabecular bone area ratio (Tb.AR) in HE staining:select10randomly in100times magnification view, trabecular bone area ratio (Tb.AR) of group B and group C were significantly higher than those of groups A and D (P<0.05); Trabecular bone area ratio (Tb.AR) of group C were significantly higher than those of groups B (P<0.05); There was no significantly difference in Tb.RA between groups A and B (P>0.05).3. The observation of scanning electron microscope (SEM):the cell ultrastructure of groups B and C of were significant different from groups A and D.Conclusion It is well that the rabbit model of tibial lengthening using the monolateral external fixation can imitate the clinical lengthening process in this study. local injection of PRP can promote bone consolidation during distraction osteogenesis effectively, especially in the middle period of distraction osteogenesis.

Related Dissertations

  1. The Effect of Hemoperfustion in Different Time on the Proinflammatory Cytokines and Survival Time of Sepsis Rabbits,R459.7
  2. MRI Guoded Experimental Cryoablation of Rabbit’s Sciatic Nerve for MRI and Pathology Contrast Study,R445.2
  3. Nutritional Evaluation and Feed Effects of Solidago Canadensis in Rex Rabbits,S829.1
  4. Experiments Study of BIO-OSS on PRP Composite Repairing Bone Defect around Metal Nail of Pure Titanium,R783.6
  5. Effect of Sciatic Injection Injury on Enzymohistochemistry of Skeletal Muscle in Rabbit’s Legs,R-332
  6. The Study of 3D Co-culture with Fibroblast and Gelatin Sponges,R329
  7. To Evaluate the Brain Iron Content by Susceptibility Weighted Imaging: Experimental Study and Clinical Application,R445.2
  8. Rabbit knee prosthesis infection and significance of changes in serum PCT levels,R687.4
  9. The Study on "Southwestern Fujian Black Rabbit" Industrial Development in ShangHang,F326.3
  10. GDF-5 and Rabbit Disc in Vitro Model Induction BMSCs of Rabbit Differentiation to the Nucleus Pulposus Cell,R329
  11. Subtalar osteotomy for treatment of old fractures slowly extended research,R687.3
  12. Chinese medicine comprehensive treatment of rabbit intervertebral disc degeneration TNF-α, MMP-3 affect the experimental study,R274.9
  13. Biodegradable Porous Silk Fibroin/hydroxyapatite/Bmp-2 for the Healing of a Rabbit Radial Segmental Bone Defect,R318.08
  14. ~ (18) F-SFB-Annexin B1 probe Experimental evaluation of apoptosis,R817.4
  15. Complex copper nano polymer composites vas deferens contraceptive device contraceptive effect experimental study,R699
  16. Experimental Studies on Fluid Resuscitation at the Early Stage of Septic Shock in Rabbits,R459.7
  17. Influence of Osmotic Loading on Organ Culture Model for Rabbit Intervertebral Disc,R-332
  18. Multiplexed Serological Diagnosis for Viral Hemorrhagic Fever by Suspension Array,R440
  19. The Effect of Subtrochanteric Varus Osteotomy to Vascularization and Endochondral Ossification of the Femoral Head in Rabbit Model of Perthes’ Disease,R687.3
  20. The Effect of Ecdysterone Combined with Mesenchymal Stem Cells on Wound Healing,R641
  21. The Effects of Aav-hbmp-2 Transfection To the Rabbit Degenerated Intervertebal Disces in Vivo on the Nucleus Pulposus of Apoptosis,R681.53

CLC: > Medicine, health > Surgery > Orthopaedic Surgery ( movement system diseases,orthopedic surgery ) > Orthopedic surgery and surgery > Bone surgery
© 2012 www.DissertationTopic.Net  Mobile