Dissertation > Excellent graduate degree dissertation topics show

Guided Tissue Regeneration Membrane Enhance Bone Formation during Distraction Osteogenesis in Rabbits

Author: TangXiaoPeng
Tutor: YuanKuiFeng
School: Shandong University
Course: Clinical Stomatology
Keywords: Distraction osteogenesis guided tissue regeneration membrane mandibular rabbit
CLC: R782
Type: Master's thesis
Year: 2012
Downloads: 35
Quote: 0
Read: Download Dissertation


Background and objective:Distraction osteogenesis (DO) is the process of bone lenghthening by gradual mechanical distracton. It was first described in the field of orthopaedics, and then developed into daily surgical practice for the treatment of different craniofacial anomalies. There are many factors that influence the quality of bone formation during distraction osteogenesis, usually the formation of fibrous tissues at the distraction gap may result from the accumulation of rapidly migrating fibroblasts at the site of an osteotomy, and then influence the bone formation by decreasing the number of osteoblasts. The guided tissue regeneration membrane (GTRM) could theoretically prevent fibroblasts from entering the distraction gap, allowing the osteotomy site to be fillde with only osteogenic cells to enhance the bone formation in distraction zone. Therefore,in this study we firstly establish the mandibular distraction osteogenesis model in rabbits, and then research the ability of guided tissue regeneration membrane in bone formation during distraction osteogenesis. The results of this study will provide valuable informations for GTRM to accelerate callus formation and shorten treatment period during distraction osteogenesis, and lay the foundation for eventual clinical application.Methods:1.Creation of the mandibular distraction osteogenesis model in rabbit (control group):Twenty white New Zealand rabbits were peformed osteotomy in the left mandible and then interal distraction device was positioned and fixed on the mandible. Five days after osteotomy, distraction was carried out at a rate of0.8mm every day(twice a day,0.4mm at8a.m.and8p.m.)for ten days, to produce a distraction gap of approximaately8mm. After distraction was completed, the distraction was left in situ, and went into the consolidation phase. Half of animal randomly chosen from each group were sacrificed at the end of week2and6throughout the consolidation phase. The right mandibular samples were taken out for X-ray and histology examinations.2.The GTRM used in distraction osteogenesis (experimental group):Twenty white New Zealand rabbits were peformed osteotomy in the left mandible and then interal distraction device was positioned and fixed on the mandible with the GTRM inside wrapped around the distraction zone, then came to the process in accordance with method one. Half of animal randomly chosen from each group were sacrificed at the end of week2and6throughout the consolidation phase. The right mandibular samples were taken out for X-ray and histology examinations.3.The distracted mandibles were removed and processed for radiographic,and then measured the bone mineral density (BMD). Eight serial sections of each specimen were made for HE staining and were observed the thickness,number and arrangement direction of the trabecular as well as the number and density of osteoblast in the light. Image analysis software was used to analyze bone histomorphometry. Eight discrete sections were measured for each specimens, and the mean measurement results of the speciments were the parameter values. The bone volumes in the newly formed cortical bone area and in the cancellous boen area of the distraction zones were measured.4.Statistical analysis:All data are expressde as menas±tandard errors in all independent experiments. Statistical differences among groups were evaluated by t-test. p<0.05was considered statistically significant. Results:1.The animals tolerated the surgical procedures and distraction well. Diet,feces and urine were normal, and the mandibular deviation occurred at the end of the distraction. Radiological and histological study confirmed osteogenesis in gaps in animal models. The mandibular were extended in accordance with the intended target. 2.Radiological and histological study confirmed that the group of GTRM (the experimental group) showed better bone formation than the control group. Results of statistical analysis on bone mineral density and histomrorphometry showed the same as the result mentioned above.(p<0.05)Conclusion:1.Our experiments establishde a feasibility,repeatability and steadily modle of rabbits unilateral mandibular distraction osteogenesis by the reformed interal distactors. It was appropriate for large quantities of animal experiments and lay the foundation for eventual clinical application combined with cytokine and material witch promoted bone formation.2.GTRM can more effectively promote new bone formation and mineralization during mandibular distraction osteogenesis under radiographic and histological examinations,suggesting that GTRM is an effective method to promote the mandibular distraction osteogenesis.

Related Dissertations

  1. The Effect of Hemoperfustion in Different Time on the Proinflammatory Cytokines and Survival Time of Sepsis Rabbits,R459.7
  2. MRI Guoded Experimental Cryoablation of Rabbit’s Sciatic Nerve for MRI and Pathology Contrast Study,R445.2
  3. Nutritional Evaluation and Feed Effects of Solidago Canadensis in Rex Rabbits,S829.1
  4. The Study of Rabbit Bone Marrow Mesenchymal Stem Cells Separation Culture and Directional Differentiation,R329
  5. Establishment of Feline Calicivirus (FCV) Reverse Genetics System Using RNA Polymerase Ⅱ and Expression of RHDV VP60 Gene,S852.65
  6. Effects of the Applied External Weak Steady Direct Current Electrical Fields on Diastolic Function after Balloon Injury in the Rabbit Abdominal Aorta,R541.4
  7. Several Low Flat Mandibular Alveolar Ridge to Improve Denture Retention Methods of Clinical Experience,R783.6
  8. Effect of Sciatic Injection Injury on Enzymohistochemistry of Skeletal Muscle in Rabbit’s Legs,R-332
  9. The Study of 3D Co-culture with Fibroblast and Gelatin Sponges,R329
  10. Clinical Analysis with Rigid Internal Fixation in 162 Cases Mandibular Fractures,R782.4
  11. \,I712.074
  12. To Evaluate the Brain Iron Content by Susceptibility Weighted Imaging: Experimental Study and Clinical Application,R445.2
  13. Experimental Study of Expression of XAF1 Following Lung Contusion in Rabbit,R655.3
  14. Experimental Study on Preventive and Therapeutic Effect of Delayed Cerebral Vasospasm after Subarchnoid Hemorrhage by Intracisternal Irrigation Using Recombinant Tissue-type Plasminogen Activator,R743.3
  15. Experimental Study of Silk Fibroin Scaffold Combined with Adipose-derived Mesenchymal Stem Cells Repairing Rabbit Tunica Albuginea Defect,R318.08
  16. Design of Positive Draw-back Motion of Wool Spinning Frame and Comparison of Prediction Models of Worsted Yarns Performances,TP183
  17. Intracisternal Infusion of Rt-PA and Urokonase in the Prevention of DCVS after Subarachnoid Hemorrhage: a Preliminary Study,R743.3
  18. Rabbit knee prosthesis infection and significance of changes in serum PCT levels,R687.4
  19. The Study on "Southwestern Fujian Black Rabbit" Industrial Development in ShangHang,F326.3
  20. The Study of the Expression of Hepatocyte Growth Factor in Mandibular Distraction Osteogenesis,R782
  21. Development of an ELISA for Detecting Cry1Ac Protein,Q78

CLC: > Medicine, health > Oral Sciences > Oral and maxillofacial surgery
© 2012 www.DissertationTopic.Net  Mobile