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Lentivirus-mediated Pygo2shRNA Inhibits Proliferation and Invasion of Kideny Carcinoma OS-RC-2Cells in Vivo and Vitro

Author: QinXiangCheng
Tutor: LiuRongFu
School: Fujian Medical
Course: Surgery
Keywords: Pygo2 MMP-7 MMP-9 VEGF
CLC: R737.11
Type: Master's thesis
Year: 2013
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Objective To study Lentivirus-Mediated Pygo2shRNA make an effect in proliferation and invasion of kidney cancer OS-RC-2cells in vitro, further Studies to verify that a role of Pygo2in tumor growth and relationship with tumor invasion and metastasis in vivo.Method Lentiviral-mediated reorganization of the three plasmids, to establish the Pygo2silence group, the Pygo2overexpression group and the corresponding control group. Lentiviral was used to infect the renal carcinoma OS-RC-2cells, and the Pygo2shRNA infection efficiency was detected by Western Blot. MTT, colony formation, cell invasion assay were studied to characterize pygo2expression in vitro. Stable cell lines were established by screening cells and four types of renal cell carcinoma OS-RC-2cells were subcutaneously inoculated in nude armpit, continuous observation of the growth of nude mice tumors and lung metastases. IHC detcted that each tumor invasive protein (MMP-9, MMP-7and VEGF).Results Pygo2shRNA effectively decreased cell proliferation, colony formation and invasion in vitro. Pygo2shRNA obviously inhibits tumor growth in nude mice xenograft model in vivo and HE staining confirmed that scattered nodules were metastatic tumors in Pygo2OE group. IHC analysis revealed that pygo2shRNA reduced the levels of matrix metalloproteinase-7(MMP-7) and VEGF in the OS-RC-2cells in vivo, however, the loss of Pygo2can not obviously decreased the expression of MMP-9, compared wih reduction of MMP-7and VEGF expression quantity phase.Conculsion Pygo2can promote the kidney cancer OS-RC-2cell proliferation and aggressive in vivo and vitro.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Urinary tumors > Kidney,renal pelvis tumor
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