Dissertation > Excellent graduate degree dissertation topics show

Effects of Transfecting Bcl-2shRNA and Celecoxib on the SGC-7901Cell Line

Author: LiuWeiWei
Tutor: ShenFangZhen
School: Qingdao University
Course: Oncology
Keywords: Bcl-2 small hairpin RNA (shRNA) RNA interference (RNAi) gene transfected celecoxib
CLC: R735.2
Type: Master's thesis
Year: 2012
Downloads: 1
Quote: 0
Read: Download Dissertation

Abstract


Objective:The aim of this study was to study the changes of SGC-7901cells transfecting small hairpin RNA (shRNA) targeted Bcl-2and celecoxib in vitro.Methods:To use the effective siRNA targeted to Bcl-2by Lipofectamine2000, the rate of cell growth inhibition of all groups was detected by MTT method, apoptosis rate was examined by flow cytometry and the expression of Bcl-2was assayed by Elisa.Results:After siRNA was transfected to SGC-7901cells, the rate of cell growth inhibition was increased, the joint group was higher by flow cytometry and the expression of Bcl-2was lower by Elisa.Conclusion:The growth of SGC-7901cells which was transfected siRNA has been inhibited, and the sensitivity to celecoxib has been increased.

Related Dissertations

  1. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  2. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  3. Biological Effects of Soluble CD40 Ligand on Leukemia HL-60 Cells and Its Mechanism,R733.7
  4. Effect of Bufalin on Proliferation and Induce Apoptosis of Vascular Endothelial Cells EAhy926,R285
  5. Effects of Dachengqi Decoction on the Expression Levels of Bcl-2 and Bax Gene of Smooth Muscle Cells of Small Intestine in Rat with Multiple Organ Dysfunction Syndrome,R285.5
  6. Effect of Clove Wei Ling Mixture on the Quantity of GAS and Bc1-2 in Rats Model of Chronic Atrophic Gastritis,R285.5
  7. Anti-cancer Effects of Tanshinone ⅡA Microemulsion for H22 Murine Hepatoma Cells Both in Vitro and in Vivo,R285.5
  8. GM-CSF in the Wound of Diabetic Rats BCL-2 Expression Levels,R644
  9. Neuroprotective Mechanism of NBP Preconditioning in Cerebral Ischemia Reperfusion Injury on Sprague-Dawley Rats,R743.33
  10. Research on Apoptosis of Human Colon Carcinoma Cell HT-29 Induced by NDGA Combined Celecoxib,R735.35
  11. The Studies to Anti-tumor Effect and Its Mechanisms of Nsaids Combined with Sodium Butyrate on Human Colon Cancer Cell Line HCT-116 in Vitro,R735.35
  12. Expression and Pathobiological Significance of Survivin, p53 and Bcl-2 Proteins in NSCLC,R734.2
  13. The Expression and Significance of CD44V6 and Bcl-2 in Eyelid Basal Cell Carcinoma,R739.7
  14. Effects of Atorvastatin Combined with Celecoxib on the Proliferation and Apoptosis of Human Colon Cancer SW-480 Cell,R965
  15. The Studies of Celecoxib Affecting Its Expression Profiles of microRNA Mechanisms on Human Colon Cancer Cell Line HT-29 in Vitro,R735.35
  16. Effect of Thalidomide on Cellular Growth and Angiogenesis of Human Pancreatic Carcinoma Cell Line SW1990,R735.9
  17. Effects Suppression of Bcl-xl Expression by RNAi on Proliferation and Apoptosis of Human Pancreatic Cancer Cells and Chemosensitivity to Gemcitabine,R735.9
  18. Effects of Proliferation and Apoptosis of Gastric Carcinoma Cell Line Sgc-7901 by Cetuximab (Erbitux),R735.2
  19. Effects of Proteasome Inhibitor MG-132 on the Proliferation and Apoptosis of Human Colon Cancer HCT-116 Cell,R735.35
  20. Clinical Significance of Dcr3, Bcl-2 and P53 Protein Expressinon in Esophageal Carcinoma,R735.1
  21. Study of the Protective Effect of Grape Procyanidins on Alcoholic Liver Injury in Mice,R285.5

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Gastric neoplasms
© 2012 www.DissertationTopic.Net  Mobile