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Effects of Transfecting Bcl-2shRNA and Celecoxib on the SGC-7901Cell Line

Author: LiuWeiWei
Tutor: ShenFangZhen
School: Qingdao University
Course: Oncology
Keywords: Bcl-2 small hairpin RNA (shRNA) RNA interference (RNAi) gene transfected celecoxib
CLC: R735.2
Type: Master's thesis
Year: 2012
Downloads: 1
Quote: 0
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Objective:The aim of this study was to study the changes of SGC-7901cells transfecting small hairpin RNA (shRNA) targeted Bcl-2and celecoxib in vitro.Methods:To use the effective siRNA targeted to Bcl-2by Lipofectamine2000, the rate of cell growth inhibition of all groups was detected by MTT method, apoptosis rate was examined by flow cytometry and the expression of Bcl-2was assayed by Elisa.Results:After siRNA was transfected to SGC-7901cells, the rate of cell growth inhibition was increased, the joint group was higher by flow cytometry and the expression of Bcl-2was lower by Elisa.Conclusion:The growth of SGC-7901cells which was transfected siRNA has been inhibited, and the sensitivity to celecoxib has been increased.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Gastric neoplasms
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