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Expression of PI3K and Akt in Salivary Malignant Tumor

Author: ZouDaWei
Tutor: XuBiao
School:
Course: Clinical Stomatology
Keywords: Salivary malignant tumor Immunohistochemistry RT-PCR PI3K/Akt
CLC: R739.8
Type: Master's thesis
Year: 2013
Downloads: 1
Quote: 0
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Abstract


Objective This study is to explore the expression of phosphatidylinositol3-kinase (PI3K) and its downstream serine threonine kinase (Akt) in adenoid cystic carcinoma and mucoepidermoid carcinoma, and its significance in the pathogenesis of salivary malignant tumor.Methods:From July2010to Janurary2013,6specimens of adenoid cystic carcinomas,14mucoepidermoid carcinomas and12benign pleomorphic adenomas were collected routinely from the Department of Oral and Maxillofacial Surgery, Affiliated Stomatological Hospital of Kunming Medical University.10specimens of non-neoplastic salivary gland near the PA (far from tumor) and ACC cell line as control. The expression of PI3K and Akt protein in the specimens were detected using immunohistochemistry and RT-PCR. The data were analyzed using Image Pro-plus6.0and SPSS17.0software package as well as the correlation between the expression of PI3K and Akt and tumor classification and histological classification. P-value less than0.05was considered as statistically significant.Results:PI3K and Akt protein located in the cytoplasm. The positive ratio of PI3K in ACC, MEC and PA were83%(5/6),79%(11/14) and17%(2/12), respectively. The positive ratio of Akt in ACC, MEC and PA were83%(5/6),64%(9/14) and17%(2/12), respectively. Non-neoplastic salivary gland near the PA and negative control groups were negative except of ACC cell line. There was a statistical difference among the expression of PI3K and Akt among the groups, the positive ratio of expression of PI3K and Akt in ACC, MEC are significantly higher than PA and no correlation between the expression of PI3K and Akt and tumor classification of MEC. The expression of PI3K and Akt has no difference of statistics between ACC and MEC.Conclusions:1. There was overexpression of PI3K and Akt in salivary gland tumor.2. The expression of PI3K and Akt in salivary malignant tumor was higher than PA and non-neoplastic salivary gland near the PA.3. PI3K/Akt signal pathway plays an important role in the process of occurrence of salivary malignant tumor.4. The expression of PI3K and Akt was no different between ACC and MEC.5. The expression of PI3K and Akt was not related to tumor classification of MEC.

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CLC: > Medicine, health > Oncology > Oral cavity, maxillofacial tumors
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