Dissertation > Excellent graduate degree dissertation topics show

The Expression and Clinical Significance of Nrf2and HO-1in Renal Carcinoma Tissues

Author: XuPeng
Tutor: LiZuo
School: Hebei Medical University
Course: Surgery
Keywords: Renal cell carcinoma Nrf2 HO-1 Immunohistochemistry RT-PCR
CLC: R737.11
Type: Master's thesis
Year: 2014
Downloads: 0
Quote: 0
Read: Download Dissertation

Abstract


Objectives: Renal cell carcinoma(RCC) is a common tumor urinarysystem,70%-80%of clear cell carcinoma, its incidence has increased in recentyears. To find a new target for early detection and prognosis and treatment ofrenal cell carcinoma has a very important significance. Oxidative stress andinflammatory response was closely connected with tumor formation and cellproliferation, and it has become a hot research for scholars of domestic andforeign, genes were related with oxidative stress pathways, may be potentialdrug targets for the treatment of cancer. The current study found that manydrugs play antioxidant and anti-inflammatory effects by regulating Nrf2/HO-1signaling pathway. To explore the Nrf2and HO-1with renal cell carcinomarelations, and to detect Nrf2and HO-1level in renal cell carcinoma andParacancerous normal tissues by immunohistochemistry SABC and RT-PCR,by immunohistochemistry SABC and RT-PCR method, discussion the role ofNrf2and HO-1in renal cell carcinoma occurrence and development.Immunohistochemistry: Protein expression levels detected Nrf2andHO-1level from paraffin embedded tissues by immunohistochemicaltechniques, in which51cases of Renal carcinoma tissues specimens,23casesof normal kidney tissue specimens. Using semiquantitative method doublescore to score, comprehensive stats staining positive rate and staining intensity,the results were analyzed by the SPSS17.0software, Nrf2and HO-1positiveexpression rate of difference between renal carcinomatissues and normalkidney tissue. Adopt χ2test, P <0.05considered statistically significant therelationship between Nrf2and HO-1adopt Pearson correlation analysis.RT-PCR detection: according kit instructions of Trizol to extract totalRNA in the frozen tissue, add RT reaction system tube, Synthesized cDNA byreverse transcription reaction, established β-actin gene fragment as internal reference of Nrf2and HO-1gene expression, and amplified in the RT-PCRMiriam, taken amplified products of each sample to make agarose gelelectrophoresis, DNA Marker (DL2000) as standard of labeled fragment, afterelectrophoresis with ultraviolet Transilluminators observed, the purposes ofelectrophoresis bands was analysised by Quantity One gel image analysissoftware, electrophoresis band of β-actin as reference, the results wasexpressed by two absorbance ratio. Absorbance ratio of the two groups adopt ttest to analyze. P <0.05was considered statistically significant.Results:1Immunohistochemistry results: Nrf2is mainly expressed in thecytoplasm of tumor cells. The expression rate of Nrf-2was82.4%in renal cellcarcinoma,34.8%in the normal renal tissue with the methods ofimmunohistochemistry. The difference was statistically significant(P=0.00,P<0.05). HO-1is mainly expressed in the cytoplasm of tumor. The expressionrates of HO-1were76.5%and39.1%in renal cell carcinoma and normal renaltissue. The difference was statistically significant(P=0.02, P<0.05).A positivecorrelation was between the expression of Nrf-2and HO-1in renal cellcarcinoma(r=0.515,P<0.05).2RT-PCR results: The expression quantity of Nrf2was0.747±0.101and0.374±0.071in renal cell carcinoma and normal renal tissue. The differencewas statistically significant(P<0.05). The expression quantity of HO-1was0.853±0.078and0.410±0.085in renal cell carcinoma and normal renaltissue. The difference was statistically significant(P<0.05).Conclusions:1The expression of Nrf-2and HO-1was higher in renal cell carcinomacompared with the normal renal tissues, which might be involved in thehappen and development of renal cell carcinoma, and played an important rolein the evolution process.2The expression of Nrf2, HO-1protein were positively correlated inrenal cell carcinoma.3The high expression of Nrf2, HO-1protein can be as valuable markers to assess the growth of renal cell carcinoma,and may be potentialtherapeutic target.

Related Dissertations

  1. GIH cDNA Cloning and Mrna Expression in Litopenaeus Vannamei,S917.4
  2. Gene Prediction and Sequence Analysis of Insect OBP CSP and sid-1,Q78
  3. Isolation, Identification and Molecular Characteristics of Japane Encephalitis Virus in Henan Province,S852.65
  4. Studies on the Difference of PRRSV Infectious and PRRSV ADE,S858.28
  5. cDNA-cloning and Expression analysis of Phosphate Stress Induced Purple Acid Phosphatase Genes and transcription Factor Genes from Seeding Wheat,S512.1
  6. Study on Key Technology and Its Physiological Basis of Virus Elimination and Rapid Propagation of Phalaenopsis,S682.31
  7. Molecular Mapping of Downy Mildew Resistant Gene and Expression of Rs-AFPs in Radish (Raphanus sativus L.),S631.1
  8. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  9. The Study of Kisspeptin/GPR54 System on the Reproductive Regulation in the Hen’s Hypothalamus-Pituitary-Ovary Axis,S831
  10. Study on the Digestive Tube During the Embryonic Stage of Gushi Chicken,S831
  11. A Comparative Study of prawns, crabs, Spiroplasma disease immunohistochemistry and pathology,S945
  12. Proliferation and Differentiation Effects of Different Culture Methods and Cell Factors on Mouse Spermatogenic Cells in Vitro,R329
  13. The Detection and Significance of CK 19 mRNA and MUC1 mRNA in Peripheral Blood in Cervical Cancer Patients.,R737.33
  14. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  15. Expressions of PRL-3 and p27 in Gastric Carcinoma and Their Clinical Significance,R735.2
  16. Expression of hnRNP A2/B1 and p21WAF1 Proteins in Lung Cancer and Their Clinical Significance,R734.2
  17. Cross-Talk between Ethylene and Heme-Heme Oxygenase Signal System in Primary Root Growth Inhibition of Etiolated Tomato Seedlings,S641.2
  18. The Effects of RIP on the Immune Function of Renewal Rats Without Colostrums,S853.7
  19. Study on the Expression and Correlation of Metallothionein and Matrix Metalloproteinase-2 in Non-small Cell Lung Cancer,R734.2
  20. Effect of HCO3-, K+ and HSO3- on rbcL and rbcS Gene Expression and Photosynthesis of Soybean Seedlings,S565.1
  21. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828

CLC: > Medicine, health > Oncology > Genitourinary tumors > Urinary tumors > Kidney,renal pelvis tumor
© 2012 www.DissertationTopic.Net  Mobile