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Stable and efficient regeneration system for transgenic technology is the foundation, but the peach is Prunus (Prunus spp.) Fruit trees in vitro regeneration more difficult species, impact studies to become a key factor of regeneration. In this study, peach cultivar 'Qin honey', 'sand red', 'sweet peach king', 'Golden Boy on the 5th' and '97 -8-4 '(Jingyu × early Red No. 2) hypocotyls explants body of different genotypes, the basic medium and plant growth regulator combinations on hypocotyl adventitious shoot regeneration in vitro effects; and peach cultivars 'fabric mesh Early', 'sweet peach king', 'Rui Jiang' and '97- 8-4 '(excellent grades), subculture plantlets leaves as materials research impact peach leaf buds regeneration factor. The main results are as follows: 1. Different varieties of peach hypocotyl regeneration rate is different genotypes obvious. The same culture conditions' sweet peach king ',' Golden Boy on the 5th 'and '97 -8-4' regeneration was significantly higher than 'Qin honey' and 'red sand'. (2) basic medium and growth regulators on the regeneration of peach hypocotyls have a greater impact. 'Sweet peach king', 'Golden Boy on the 5th' and '97 -8-4 'are in the LP medium regeneration rate and the highest number of shoots, MS medium followed, G regeneration medium worst effect; hypocotyl regeneration, plant growth regulating substances used 2.0 mg / L BA with 0.02 mg / L NAA regenerate better, 'sweet peach king', 'Golden Boy 5' and '97 -8-4 'next embryo Axis regeneration rate reached 65.79%, 68.44% and 68.98%. 3 genotype on peach leaf callus induction and shoot regeneration has a great influence. '97 -8-4 'And' sweet peach Wang 'leaf callus induction rate of up to 100%, adventitious bud regeneration rate was also higher, respectively, up 18.33% and 16.67%;' fabric mesh Early 'regeneration rate is low, 10%; 'Ruijiang' can not be regenerated adventitious buds. 4 The most basic suitable callus induction medium for MS, the most suitable for shoot regeneration of the basic medium for LP. '97 -8-4 'Leaves in MS, LP and G in three media can induce callus, and the highest rates of MS in the induction, LP followed, but in the LP, the highest rate of adventitious bud regeneration can be up to 18.33%, significantly higher than MS and G. 5 leaves of different maturity adventitious shoot regeneration ability of different, fully expanded leaves callus induction works best shoot regeneration rates were the highest, up to 96.67% and 18.33%. Not fully expanded leaves callus induction rate is higher, reaching 83.33%, can be regenerated adventitious buds. Unexpanded leaves and mature leaves callus induction rate, and not longer give birth buds. 6 leaf buds produced in different parts of different capacities, the highest rate of shoot regeneration petiole, leaf base and leaves the middle, followed by the upper blade can not be regenerated adventitious buds. 7 cytokinin concentration is fixed, with NAA concentration, leaf callus induction rate and the amount of each of the blades produced callus also showed an upward trend overall, the higher the concentration the more compact callus structure. 8 leaves dark cultured optimal growth regulator combination 2.0 mg / L BA 0.2 mg / L NAA; optical cultured optimal growth regulator combinations of 4.0 mg / L BA 0.1 mg / L NAA or 6.0 mg / L BA 0.2 mg / L NAA. '97 -8-4 ',' Sweet peach king 'and' cloth mesh Early 'fully expanded leaves in the optimal culture conditions shoot regeneration rate was 18.33%, 16.67% and 13.33%.
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