Dissertation > Excellent graduate degree dissertation topics show

Association of the TGF-β1 Gene Polymorphism with the Recurrent Spontaneous Abortion

Author: LiXia
Tutor: HuoZhengHao
School: Ningxia Medical University
Course: Genetics
Keywords: Transforming growth factor- β1 (TGF-β1) Gene polymorphism Haplotype Recurrent spontaneous abortion (RSA) Hui Han
CLC: R714.2
Type: Master's thesis
Year: 2011
Downloads: 24
Quote: 0
Read: Download Dissertation

Abstract


The purpose of transforming growth factor β1 (transforming growth factor-β1, TGF-β1) is a pluripotent cytokine, plays an important role in the development process in a variety of diseases. The four polymorphisms points of this study by the first exon of the TGF-β1 gene promoter region (SNP-800G / A, SNP-509C / T) and the promoter region (SNP 869T / C, SNP 915G / C) detection, analysis of the four SNP loci gene polymorphisms of TGF-β1 gene haplotypes constructed extremely Ningxia Han women with recurrent spontaneous abortion (of Recurrent spontaneous abortion, RSA) correlation. Method case group and 228 cases taken from Ningxia reproductive and genetic Key Laboratory confirmed recurrent spontaneous abortion patients, average age 27.89 ± 4.42 years; choose age-matched normal pregnant women in Yinchuan City, MCH hospital delivery 213 cases as a control group. , with an average age of 27.56 ± 3.60 years. By polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) to detect the TGF-β1 gene the four polymorphism loci genotypes, and the chi-square test and the risk of disease is estimated using the SPSS 13.0 statistical software four SNP loci linkage disequilibrium and haplotype build completed by SHEsis software. Results ① TGF-β1 gene polymorphism four sites genotypic distribution Hardy-Weinberg equilibrium law, selected population group representation. ② TGF-β1 gene SNP-509 C / T TT genotype distribution of frequency in the case group was significantly higher than that in the control group, the difference was statistically significant (P = 0.028, OR = 1.651), while the CC and CT genotypes between the case and control groups, the difference was not statistically significant (P = 0.102, P = 0.633); distribution frequency of the T allele in the case group was significantly higher than that in the control group, the low frequency of the C allele in the case group in the control group, the difference was statistically significant (P = 0.022, OR = 1.363; P = 0.022, OR = 0.734). The three polymorphisms of TGF-β1 gene SNP-800G / A SNP 869T / C SNP 915G / C genotype frequency and allele frequencies between the case and control groups showed no significant difference (P gt; 0.05). (3) between any two of the the four polymorphisms points of the TGF-β1 gene chain imbalance (D 'gt; 0.8). ④ TGF-β1 gene SNP-800G / A SNP-509C / T SNP 869T / C and SNP 915G / C polymorphisms of four points to build haplotype seven protective haplotype in the case group The control group was statistically significant (P lt; 0.05, OR lt; 1); significant difference (P lt; 0.05, OR gt; 1) three risk haplotype distribution in both groups. Conclusion 1, TGF-β1 gene SNP-800 genotype and allele frequencies of the SNP 869, SNP 915 polymorphism of three points in the distribution in the case and control groups had no statistical significance (P gt; 0.05 ), susceptibility and Ningxia Han women with recurrent spontaneous abortion. 2, TGF-β1 gene SNP-509C / T polymorphism with recurrent spontaneous abortion association, its TT genotype may be predisposing factors Ningxia Han women with recurrent spontaneous abortion, and at the same time carrying T allele significantly higher risk of recurrent spontaneous abortion. 3, TGF-β1 gene four mutation sites pairwise linkage disequilibrium. Haplotypes of TGF-β1 gene constructs, seven kinds of single haplotype protective factors Ningxia Han women with recurrent spontaneous abortion, three single haplotype Ningxia Han women with recurrent spontaneous abortion risk factors. Objective To investigate the transforming growth factor-β1 (transforming growth factor-β1 and TGF-β1) gene promoter region SNP-800 (G / A), SNP-509 (C / T) and the first exon of the promoter region SNP 869 (T / C), SNP 915 (G / C) four polymorphisms points in the Ningxia Hui and Han population genotype frequencies and allele frequency distribution characteristics, and compare their distribution in different regions and ethnic differences. Transforming growth factor β1 promoter region (-800 locus method using polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) Hui 210 cases and 308 cases of Chinese Han population, -509 locus) and the first exon region (869 points, 915 points) four polymorphic loci detected. Results Ningxia Hui population the four polymorphisms points of the TGF-β1 gene genotypes and allele frequencies were: -800 (GG: 97.62%; GA: 2.38%; AA: 0%; G: 98.81%; A: 1.19%); -509 (CC: 21.90%; CT: 56.67%; TT: 21.43%; C: 50.24%; T: 49.76%); 869 (TT: 25.24%; CT: 52.38%; CC: 22.38 %; T: 51.43%; C: 48.57%); 915 (GG: 98.10%; GC: 1.90%; CC: 0%; G: 99.04%; C: 0.96%). Han population in Ningxia the four polymorphisms points of the TGF-β1 gene genotype and allele frequencies were: -800 (GG: 99.03%; GA: 0.97%; AA: 0%; G: 99.52%; A : 0.48%); -509 (CC: 27.27%; CT: 50.97%; TT: 21.75%; C: 52.76%; T: 47.24%); 869 (TT: 30.52%; CT: 48.05%; CC: 21.43% ; T: 54.55%; C: 45.45%); 915 (GG: 99.03%; GC: 0.97%; CC: 0%; G: 99.51%; C: 0.49%). Between the two groups were not statistically different (P gt; 0.05). Grouped by gender, the four polymorphism loci genotype and allele frequencies and no statistically significant difference (P gt; 0.05). Conclusion: TGF-β1 gene -800, -509, 869, 915 four polymorphism loci in Ningxia Hui and Han populations and different gender, no significant difference, but a significant difference with other regional and ethnic groups .

Related Dissertations

  1. Polymorphisms of Y-STRs in Uygur and Kazak Ethnic in Xinjiang,R394
  2. Chinese Young Men Waist-dimensional CT 4,5 Vertebral Pedicle Morphology and Clinical Significance of the Difference,R687.3
  3. Association of Interleukin-6 Gene Polymorphisms with Susceptibility to Coronary Heart Disease in Asian Populations: a Meta-analysis,R541.4
  4. Chifeng City in parts of Alzheimer's disease and blood lipids preliminary analysis of trace elements,R749.16
  5. Jingmen study Hui and traditional morality,B82
  6. The Research about Hui Nationality of Hebei Genealogy,K248
  7. The Research on the Tactics Comparison of Chinese Dynasty and Northern Ethnics in Qin and Han Dynasty,K232
  8. Folk Beliefs of Mongolian and Han in Immigration Confluence Moving Westward,B933
  9. Research on the Rural Poverty in Linxia Hui Autonomous State,F323.8
  10. Chinese and Uighur Group Financial Control,F406.72
  11. The Investigating Study on Intermarriage between Hui and Hans,C95
  12. Bafang Hui Community Social Assistance Research,D632.1
  13. Urbanization Business Culture Research on Hui Ethnic Group,C95
  14. Study on the Han Traditional Costumes in Areas of Kunming,J523.5
  15. Verification on Quotations from Mao Shi by the Sound and Meaning of the Tripitaka. Compiled by Xuan Ying,I207.22
  16. The Correlation Research between the Gene Polymorphisms of Five CYP450 Isoforms of Enzymes and the Differences of Metabolic Ratio of Cocktail Probes,R969.1
  17. CHD patients with depression nitric oxide synthase gene G894T polymorphism,R749.4
  18. Analysis of Related Factors and Outcomes of Gestational Diabetes Mellitus between Different Nationalities in Xinjiang,R714.256
  19. Polymorphism Study of Tumor Necrosis Factor Alpha (TNF-α) in Dermatomyositis Patients,R593.26
  20. The Relationship Between Tumor Necrosis Factor-βrs1041981 Gene Polymorphisms and Coronary Artery Disease in the Patients of Nanjing Area,R541.4
  21. Polymorphism of Killer Cell Immunoglobulin-like Receptors Gene in Sichuan Han Population, China,R394

CLC: > Medicine, health > Obstetrics and Gynaecology > Obstetrics > Pathological pregnancy ( abnormal pregnancy )
© 2012 www.DissertationTopic.Net  Mobile