Dissertation > Excellent graduate degree dissertation topics show

Effects of SCF on Morphology and Electrophysiology of Interstitial Cells of Cajal-like Cells in the Bladder in the High Glucose Environment

Author: LiuXinJun
Tutor: WangQinZhang
School: Shihezi University
Course: Surgery
Keywords: Interstitial Cajal -like cells SCF Calcium Ultrastructure
CLC: R694
Type: Master's thesis
Year: 2011
Downloads: 16
Quote: 0
Read: Download Dissertation

Abstract


Objective: To observe the stem cell factor (stem cell factor, SCF) guinea pig bladder after the intervention of high-sugar environment Cajal-like interstitial cells (the interstitial cells of Cajal, ICCs) morphological, ultrastructural and electrophysiological changes discussed SCF high-sugar environment under the guinea pig bladder ICCs-like cells. Method: experimental collagenase digestion method separation cultured guinea pig bladder ICCs-like cells into normal control group (glucose concentration of 5 mmol / L), high glucose group (glucose concentration of 15mmol / L and 30mmol / L), medium culture after 72h high glucose group was divided into high-sugar control group, SCF50ng/mL the group, SCF100ng/mL group, cultured for 24h and 72h. Using transmission electron microscopy ultrastructural changes in the groups different time periods ICCs-like cells; using laser scanning confocal microscope to measure the length of ICCs-like cells of different time periods in each group and record changes in intracellular calcium fluorescence intensity values. Results: With the incubation time, by transmission electron microscopy and laser scanning confocal microscopy showed ICCs-like cell ultrastructure of the normal control group, no significant differences in calcium fluorescence values ??and cell length. 15mmol / L high glucose group: high-sugar control group than the normal control group, the number of ICCs-like cells organelles significantly reduced mitochondrial swelling, of varying sizes, vacuolar degeneration or even dissolve, endoplasmic reticulum, cytoplasmic widely dissolved extracellular weeks processes shorter and even disappear, a significant decrease in intracellular calcium fluorescence value, cell length was significantly shorter (P lt; 0.01). SCF group with incubation time and SCF concentration ICCs-like cells ultrastructure gradually improved, an increase in the number of organelles, morphology tends to the normal control group, the protrusion number of cellular weeks. ICCs-like cells calcium fluorescence value SCF50ng/mL for 24h and cell length higher sugar control group no difference (P gt; 0.05), SCF50ng/mL role in cells after 72h increased calcium fluorescence values ??higher sugar control group ( P lt; 0.05), the calcium fluorescence values ??SCF 100ng/mL role 24h after a high-sugar control cells increased cell length increased (P lt; 0.05), SCF 100ng/mL role of intracellular calcium fluorescence after 72h. high-sugar control group significantly increased cell length was significantly increased (P lt; 0.01). 30mmol / L glucose group: high-sugar control group compared with 15 mmol / L high-sugar control group, the number of intracellular organelles further reduced mitochondrial swelling, dissolution, endoplasmic reticulum dilated, wide cytoplasm 'Pan dissolved, and a large number of vacuolization, cell weeks protrusions disappear. Compared with the normal control group, a significant reduction in intracellular calcium fluorescence value, cell length was significantly shorter (P lt; 0.01). SCF group ICCs-like cells ultrastructure higher sugar control group no significant changes in the the SCF50ng/mL role of 24h and 72h and SCF100ng/mL of role after 24h ICCs-like cells calcium fluorescence value and cell length higher sugar control group, no difference ( P gt; 0.05), SCF100ng/mL role after 72h increased calcium fluorescence values ??higher sugar control group (P lt; 0.05). Conclusion: exogenous SCF interventions after the environment caused by high sugar ICCs like cell morphology, ultrastructure and electrophysiological abnormalities improvement or reversal of role, for the rich diabetic bladder disease (diabetic cystopathy, DCP) pathogenesis provides a theoretical basis for looking for new treatments.

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. Study on the Procees for the Preparation of Prohexadione and Its Analogs,TQ452
  3. Coparative Analyses of Carbon and Nitrogen Metabolism of Flue-cured Tobacco Growing in Henan and Yunnan,S572
  4. Kinase Activities Analysis and Localization of ZmCCaMK Induced by Abscisic Acid in Leaves of Maize Plants,S513
  5. Dynamic Effect of Cold Restraint Stress on Damage of Ultrastructure and Expression of Heat Shock Protein in Rat Tissues,S858.91
  6. The Effect of High-level Copper and Zinc Addition on the Performance, Digestibilities and Gastrointestinal Enzyme Activities of Weaned Piglets,S828.5
  7. Effects of Long-term Located Fertilization on Forms Distribution of Soil Calcium in Protected Field,S158
  8. Changes of Cell Ultrastructure and Physiological Indexes and Physical Properties of Tobacco Leaves during Bulk Flue-Curing,S572
  9. Effects of Ginsenoside Rg1 on Cell Proliferation, Secretion Function and ERK、p38 Signal Molecular in Human Umbilical Cord Blood Mesenchymal Stem Cells under Optimized Culture Condition,R285.5
  10. Study on Knowledge, Attitude, and Behavior to Calcium Nutrition Among Patients with Hypertension in a Community,R473.2
  11. Roles of Small Conductance Calcium-activated Potassium Channels in Mice with Alzheimer’s Disease,R749.16
  12. Different rice varieties to brown planthopper resistance mechanism Preliminary Study,S511
  13. Lily obstacles to overcome before fertilization methods and anther chloroplast development and fertility related research,S682.29
  14. The Difference Analysis of gene Expression in Lonicera Confusa DC.under Calcium-rich Environment by GeneFishing,S567.79
  15. Effect of Hydrogen Peroxide and X-ray on Calcium Oscillation in Airway Epithelial Cells and Pulmonary Macrophages,R563.9
  16. The Effect of Smear Layer on the Antibacterial Activity of Garlicin and Calcium Hydroxide in Vitro,R783.5
  17. A Study on Marketing Research of Calcium Food in Xinjiang Area and Marketing Development Strategies of TF Co.,F426.82
  18. Influence of Different Leves of Dietary Potassium on Digestibility and Metabolism of Nutrients and Serum Calcium Balance Status of Xinjiang Finewool Sheep,S826.5
  19. Mutagenic Effect of Satellite Carrying for Trifolium Repens,S541.2
  20. Study on A Novel Injectable Vertebral Bone Cement of Calcium Phosphate and Calcium Sulfate,R318.08
  21. Nitric oxide donating dihydropyridine derivatives,R914

CLC: > Medicine, health > Surgery > Urology ( urinary and reproductive system diseases) > Bladder disease
© 2012 www.DissertationTopic.Net  Mobile