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Influence of Salvia Miltiorrhiza Beg.f.Alba Pharmacological Preconditioning on BCL-2 and Bax of Middle Cerebral Artery Obstruction

Author: WuYuePeng
Tutor: NiuJingZhong
School: Taishan Medical College
Course: Geriatrics
Keywords: Alba Pharmacological preconditioning Middle cerebral artery occlusion model Bcl-2/Bax
CLC: R285.5
Type: Master's thesis
Year: 2011
Downloads: 12
Quote: 0
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Abstract


Objective To observe the water alba root extract pretreatment pretreated with different concentration of focal cerebral ischemia (Middle cerebral artery obstruction, MCAO) neurons of Bcl- 2 and Bax protein expression affect . Explore Salvia miltiorrhiza root aqueous extract dose feasibility of its role as a pharmacological preconditioning was to reveal the mechanism of the neuroprotective effect of Salvia miltiorrhiza . Research methods healthy adult Wistar rats were randomly divided into alba high , medium and low concentration group and the control group , sham operation group , respectively, by alba , the low concentration of water extract and distilled water , distilled water intragastrically 7 days , the rat model of focal cerebral ischemia using the suture method . Middle cerebral artery occlusion model replication after 24h rat brain tissue using immunofluorescence microscopy to detect apoptosis-related proteins Bax and Bcl-2 expression . The immunofluorescence detection results : Bcl -2 protein staining was mainly located in cytoplasm of nerve cells , the nuclear envelope and protrusions for green fluorescence . The fluorescence intensity of the sham group is extremely low ; low fluorescence intensity of the blank control group ; compared with the blank control group , alba high , medium and low concentrations of fluorescence intensity increased (P lt; 0.05). And with the concentration increased , the fluorescence intensity increased gradually between the various concentrations of the same there is a significant difference ( P LT ; 0.05 ) . Bax protein staining was mainly located in the cytoplasm of the nerve cells , the nuclear envelope and protrusions , and for green fluorescence . The fluorescence intensity of the sham group is extremely low ; stronger fluorescence intensity of the blank control group ; compared with the blank control group , alba high , medium and low concentrations of fluorescence intensity lower ( P lt; 0.05 ) . And with the concentration increased , the fluorescence intensity gradually reduce , among concentration groups , there are also significant differences ( P lt; 0.05 ) . Conclusions . Different concentrations of Salvia miltiorrhiza aqueous extract pretreatment significantly reduced the number of apoptotic cells , has a protective effect on focal cerebral ischemia in nerve cells , and a dose-dependent manner . The mechanism of action may be associated with increased expression of Bcl-2 , to reduce the expression of Bax .

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