Dissertation > Excellent graduate degree dissertation topics show

Studies of Different Therapeutic Methods on Airway Inflammation and Remodeling in the Mice Asthmatic Model

Author: HanZhengLi
Tutor: QuZhengHai
School: Qingdao University
Course: Pediatrics
Keywords: Matrix Metalloproteinases-9 IL-4 INF-γ Ginseng-Schisandrae Drug decoction Xiao qinglong decoction
CLC: R725.6
Type: Master's thesis
Year: 2011
Downloads: 21
Quote: 0
Read: Download Dissertation

Abstract


Objective To study the effect of different theraputic methods on airway inflamma-tion and remodeling in the mice asthmatic model.Methods 60 healthy female Kunming mice were divided into six groups randomly, 10 mice of each group, normal control group (A),asthma group (B),Pulmicort group (C),Ginseng-Schisandrae Drug decoction group(D),Xiao qinglong decoction group (E),two step sequential method group(F).The mice of B、C、D、E、F groups were established the models of asthma using ovalbumin,after building,each group was given different therapy.Then observe the pathological change in lung tissue.Finally assay the levels of IL-4、INF-γin BLAF with enzyme-linked immunosorbent assay (ELISA) and detect the level of MMP-9 and TIMP-1 of lung tissue with immunohis-tochemical analysis.Results①There were no wheezing symptoms in group A while there were wheezing symptoms in other groups and the wheezing symptom of group B was more serious than the treatment groups.There was no significant difference among the treatment groups.②Pathological analysis of the lung showed that group A had a little inflammatory cell infiltration,which was significantly lower than that of other groups(P<0.05) and group B had a large number of inflammatory cells infiltrating around tracheas, which was significantly higher than other groups(P<0.05); There was no significant difference among the treatment groups(P>0.05).③there was no significant difference between group A and group F(P>0.05); there was significant difference among other groups (P<0.05);④The level of IL-4 in BALF was significantly lower in group A than that of other groups (P<0.05) while the level of IL-4 in BALF was significantly higher in group B than that of other groups(P<0.05) and there was no significant difference among the treatment groups(P>0.05).⑤The level of TIMP-1 of lung tissue was lower in group A than that of group B、D (P<0.05) and group B was higher than that of other groups(P<0.05), group D was higher than group E(P<0.05), which had significant difference among the above groups (P<0.05); there was no significant difference among other groups(P>0.05).⑥The level of MMP-9 of lung tissue and the ratio of MMP-9 and TIMP-1 were lower in group A than that of other groups while that of group B were higher than other groups (P<0.05); there was no significant difference among the treatment groups.Conclusions①The model of asmatic mouse stimulated by OVA may be resultd from the unbalance of Th1/Th2 by detecting the unbalance of IL-4 and INF-γwhile the unbalance of MMP-9/TIMP-1 may demonstrate that there was airway remolding at early period.②Traditional chinese medicine may reduce airway inflammation and airway remolding by adjusting the expression of the unbalance of Th1/Th2 and MMP-9/TIMP-1 respectively, which can be got the same result by using pulmicort.

Related Dissertations

  1. Inhibition of RAW264.7 Macrophage Inflammatory Cytokines Release by Guggulsterone,R285
  2. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  3. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  4. Cloning and Expression Analysis of NCCRP-1 AND IL-10 Genes in Grass Carp (Ctenopharyngodon Idellus),S917.4
  5. Study on Introduction of Oncolytic Adenovirus with IL-24 Gene into Acute Myeloid Leukeima Stem Cell Through CD123,R733.7
  6. Relationships with primary hypothyroidism and inflammatory mediators in serum Aβ40 and brain dysfunction,R581.2
  7. Clinical Applying of Urinary IL-18 Level in Preterm Neonates with acute Kidney Injury,R722.6
  8. The Therapeutic Role of Adenovirus Vector Mediated Rat Interleukin 10 (rIL-10) Gene to Streptozotocin Induced Type 1 Diabetes Mellitus of KM Mice,R587.1
  9. Effect of AMSCs Compound Chitosan/Gp/collagen Ⅰ Transplantation on TNF-α 、IL-1β Content in Early Degenerated Intervertebral Disc of Rabbit,R681.53
  10. Regulating qi and blood Tonga catgut implantation treatment of chronic urticaria clinical research and for IL-2, IL-4 levels in,R275.9
  11. The Cross-talking between Decidual γδ T Cells and Trophoblasts in Human Early Pregnancy,R339.22
  12. Construction of the Recombinant of HSV-2 gD Minotope and IL-18 DNA Vaccines and Its Inducing Immune Respons in Mice,R392
  13. Circulating Th17 Cells Frequency is Associated with the Disease Progression in HBV Infected Patients,R512.62
  14. The Study of Levels of Toll-like Receptor 9 and IL-10、 IFN-α in Patients with Systemic Lupus Erythematosus,R593.241
  15. The Roles and Mechanisms of Methotrexate or Simvastatin on Th17/IL-17 in Peripheral Blood Mononuclear Cells from Rheumatoid Arthritis in Vitro Study,R593.22
  16. Association between Serum Fetuin-A and Proinflammatory Cytokines in Patients with Chronic Renal Failure.,R692.5
  17. Experiment Studies on the Effects of MMP-2 Expression Induced by Titanium Particles in MC-3T3-E1 Cells in Vitro,R687.4
  18. Local Administration of Interleukin-1 Receptor Antagonist Intervening Achilles Tendon Contracture in Rats: A Empirical Study,R686
  19. Glucocorticoid treatment of bronchial asthma patients serum IL-18, IL-33 levels in,R562.25
  20. Osteopontin through the MAPK signaling pathway and increased MMP9 expression correlation with embryo implantation,R321
  21. PNS treatment of rabbit lung contusion in rats,R285.5

CLC: > Medicine, health > Pediatrics > Children within the science > Department of pediatric respiratory and chest diseases
© 2012 www.DissertationTopic.Net  Mobile