Dissertation > Excellent graduate degree dissertation topics show

Screening of Strain Producing β-Glucosidase and Studies on the Induction Effects of Corncob

Author: WangChunLi
Tutor: ChenChang
School: Beijing University of Chemical Technology
Course: Biochemical Engineering
Keywords: β - glucosidase Aspergillus niger Protoplast Mutagenesis Breeding Corn cob Induction
CLC: Q933
Type: Master's thesis
Year: 2010
Downloads: 131
Quote: 1
Read: Download Dissertation

Abstract


β-glucosidase enzyme is one of the cellulase components can be removed in the process of cellulose degradation cellobiose inner the endoglucanase and outer endoglucanase inhibited so as to effectively improve the cellulose degradation efficiency. In addition, the enzyme was also used in the juice flavoring food ingredients detoxification. In this study, by UV mutagenesis and protoplast Breeding of Aspergillus niger beta-glucosidase producing strains. The mutant was carbon and nitrogen source optimized corn cob can significantly promote the secretion of the enzyme, and preliminary study corncob induced β-glucosidase. The main results obtained are as follows: 1. Plantarum CGMCC Aspergillus niger 3.316 as the starting bacteria, by ultraviolet mutagenesis screening to obtain an enzyme activity increased by 15% of Aspergillus niger 3-3M passaged good stability. 2 to determine the conditions of release and regeneration of 3-3M protoplasts of Aspergillus niger. The mycelium using solid cellophane plate method after 24 h of culture, 0.6 M ammonium chloride / 0.1 M, pH6.0 sodium citrate buffer as osmotic stabilizer. Broken enzyme combination the snail enzyme cellulase the concentrations are 5mg/mL, hydrolysis temperature 32 ° C treatment for 4 h. Aspergillus niger, 3-3 M protoplast regeneration with the flat plate the dumping law hypertonic Czapek medium culture, the regeneration rate of 90% can be obtained. 3-3M Aspergillus niger as the original strain by protoplast UV mutagenesis, screened strains of β-glucosidase activity was 23.4 IU / mL mutagenic strains 60B-3D, its enzyme activity than the 3-3M 23 %. Proven its genetic stability. Study of Aspergillus niger fermentation characteristics of 60B-3D and comparison with the original strain (CGMCC 3.316 and Aspergillus niger 3-3M), the results show that: the excellent characteristics of 60B-3D Aspergillus niger fermentation 60h revealed than the starting strain has more protein secretion capacity. Preliminary optimization of Aspergillus niger 60B-3D carbon and nitrogen sources, the results show that the best carbon source for the corn cob, the best nitrogen source for ammonium sulfate and yeast beta-glucosidase activity was the highest reached 63.86 IU / mL. 5 compares the different carbon sources, the results show that the effect of the secretion of beta-glucosidase from Aspergillus niger CGMCC 3.316 corncob of the best, which is consistent with the conclusion in Aspergillus niger 60B-3D. On this basis, explore a corn cob induced β-glucosidase. The results show that the water soluble component (WS) in the corn cob can be significantly induced the secretion of β-glucosidase, little influence of the concentration of WS its effects; water insoluble component (WIR) is also conducive to the synthesis of β-glucosidase enzyme.

Related Dissertations

  1. Study on DSP-Based Four-Quadrant Control System of Induction Motor,TM346
  2. Research on Vector Control System of Induction Motor Based on DSP,TM346
  3. Study on Rice-Frog and Melon-Frog-fish Ecological Cultivation and Breeding Mode and the Nutrient Balance,S-0
  4. Breeding of High-Yield Pigment Production Monascus and Studies on Pigments’ Qualities,TS202.3
  5. Study on Rapid Propagation of Energy Plant Euphorbia Tirucalli,Q949.93
  6. Expression of Glucose Oxidase Gene from Aspergillus Niger Z-25 in Pichia Pastoris,Q78
  7. Research on the Quility of Pear and Soil Physical and Chemical Properties in Crop-Animal Mixed Orchard,S661.2
  8. An Experiment Study About Isolation、culture of Mouse Adipose-derived Stem Cells in Vitro and Factors’ Secretion Related to the Hematopoietic Regulation of It and After It’s Induced to Osteoblasts,R329
  9. Research on the Mutation Breeding of Flavomycin and Regulation of Fermentation Techniques,TQ920.6
  10. Evaluation of Heavy Metals in Livestock and Poultry Breeding Industry Abstract,X830
  11. Association Analysis between SSR Markers and Major Agronomic Traits in Cultivated Soybean (Glycine Max L. Merr.),S565.1
  12. Studys of the Protein Extract and Utilization of Producing Protein Feed from Tea Residues,S816
  13. Mutation Inducing Breeding of Avilamycins Production and Optimization of Fermentation Conditions,TQ927
  14. Breeding of High-Yield Nosiheptide Strain and Studying of Its Formation Condition,TQ936.16
  15. Breeding and Utilization of the Genetic Male Sterile Line in Purple Stalk Chinese Cabbage,S634.6
  16. Studies on Breeding of Tetraploid in Pakchoi (Brassica Rapa L. Ssp. Chinensis L.) Based on Microspore Culture,S634.3
  17. An Analysis of the Fodder Purchasing Conduct of Egg Farmers in the Food Safety Visual Angle,F326.3
  18. Genetic Diversity in Different Populations of Tilapia (Oreochromis) by Microsatellite DNA Analysis,S917.4
  19. Analysis of Game Logic Reasoning in the Field of Economic,B812
  20. Cloning and Expression of Novel β-Glucosidase Genes from Rhizopus Stolonifer Var. Reflexus,Q78
  21. Investigation on the Freshwater Fish Breeding and Processing Security in Hunan Province,S964

CLC: > Biological Sciences > Microbiology > Microbial Genetics
© 2012 www.DissertationTopic.Net  Mobile