Dissertation > Excellent graduate degree dissertation topics show

Study on the Extraction and Bioactivity of Malus Asiatica Nakai Polyphenols

Author: ShiYunJie
Tutor: XiongHeJian
School: Jimei University
Course: Agricultural Products Processing and Storage
Keywords: Malus Asiatica Nakai Polyphenols purification Preparative HPLC Biological activity
CLC: TQ914.1
Type: Master's thesis
Year: 2012
Downloads: 30
Quote: 0
Read: Download Dissertation

Abstract


This paper took air-dried Malus Asiatica Nakai leaves as experimental material, responsesurface methodology (RSM) was employed to optimize the extraction process of Malus AsiaticaNakai Polyphenols; Evaluated it‘s scavenging free radicals and antioxidant properties;Investigated the effect of Malus Asiatica Nakai Polyphenols on the microorganisms and cancercells in vitro experiments, Discussed the potential of Malus Asiatica Nakai Polyphenols asoil antioxidant; Established analysis and compound purification methods by HPLC. It providestechnical supports for exploitation and process of Malus Asiatica Nakai Polyphenols in the future.The main results are as following:On the basis of single factor experiments, response surface methodology (RSM)experimental design was employed to establish the mathematical models of Malus AsiaticaNakai Polyphenols extraction. The optimum conditions were as follows: ethanol volume ratio:60%, extraction time:60min; liquid/material ratio:13:1. Under this condition, extraction rate ofpolyphenol from Malus asiatica Nakai leaves was (4.61±0.035)%(N=3). It was closed to themodel predicted value4.74%. The RSD between them was0.03%, which revealed the validity ofthe model.Malus Asiatica Nakai Polyphenols exhibited a high scavenging activity against DPPHradicals, superoxide anions radicals and hydroxyl radicals, and the IC50of Malus Asiatica NakaiPolyphenols equivalent to32.2%gallic acid was17.2μg/mL,203.25μg/mL,82.88μg/mLrespectively. The total antioxidant capacity was68.98±3.41U/mg, which imply that it have avery powerful antioxidant capacity. The antibacterial test showed that the Malus Asiatica NakaiPolyphenols can inhibit the growth of Bacillus subtilis and E. coli, however, have no significanteffect on Staphylococcus aureus; Malus Asiatica Nakai Polyphenols with a concentration of1mg/mL promoted the growth of BGC-823and HeLa cells by MTT assay. The increase rate was45.5%and49.61%respectively; The edible oils which added sufficient Malus Asiatica NakaiPolyphenols show non different with iodine value and acid value compared to the blank one inthe simulation of the oxidation test, which indicated that the Malus Asiatica Nakai Polyphenolsmay not suitable as antioxidants additive for edible oil directly. Preparative high performance liquid chromatography condition: Column: Boston GreenODS-5μm-4.6mm ID×250mm. Conditions:280nm.1mL/min. A=0.2%formic acid (v/v) inwater, B=Methanol. Gradients(min/B):-46/100，-31/100，-30/5，0/5，15/30，40/40，60/50，65/55，90/100； Sample: saturated100μL Malus Asiatica Nakai Polyphenols in5%methanol aqueous (v/v) solution. Fraction collection setting: collect by peak; Detection channel:280nm; Delay volume:221μl; Peak start threshold:10.00mAU; Peak start slope:0.500mAU/s;Peak start true Time:1.00s; Peak end threshold:5.00mAU; Peak end slope:-1.000mAU/s;Peak end true time:1.00s; Deriv step:1.00s; Threshold no peak end:2000.00mAU; Under thiscondition, it had collected6fractions of the Malus Asiatica Nakai Polyphenols.

Related Dissertations

  1. Study on Protective Effects of Apple Polyphenol on Immune System Injury Caused by γ-Ray,S661.1
  2. Study on Wastewater Purification Effect of the Free Surface Wetland with Different Substrates and Plant Combination,X703
  3. Penicillium Expansum TS414 Lipase in Pichia Pastori: Expression, Purification and Enantioselective Esterification of (R, S)-naproxen,Q814
  4. Studies on Fermentation Optimization, Purification and Enzyme Characteristics of Lipase from Aspergillus Oryzae FS-1,TQ925.6
  5. Studies on Isolation, Purification and Chemical Structure for Polysaccharides from Camellia Chrysantha(Hu) Tuyama,S567.19
  6. Research of Glycosaminoglycan from Perna Virids Effect on Blood Lipid,R285.5
  7. Study on the Secondary Metabolites of Two Fungi from the South China Sea,R284
  8. Purification of Endogenous Proteases from (Litopenaeus Vannamei) Shrimp Head and Their Enzymology Properties,S985.21
  9. Study on the Preparation of the Low Molecular Weight Fuciodan from Sargassum Henslowianum (C.Agardh) and Anti-tumor Activity,TS254.9
  10. Study on Extraction and Active Function of the Chemical Composition of Cacumen Platycladi,R284
  11. Under microaerobic conditions closed calcium carbide furnace exhaust COS gas adsorption purification,X781
  12. Studies on Extraction and Analysis of Fructooligosaccharides in Smallanthus Sonchifolius,TS255.1
  13. The Antioxidative Efficient Component Recognition for Curcuma Longa L. Based on the Quality Control Pattern of Constituent Knock-out/Knock-in,R285
  14. Experiment Study of the Biogas Purification Process by Zeolite Filling Washing,TQ221.11
  15. Optimization of Fermentation Conditions, Purification, Cloning and Expression of a Cold-active Lipase from Pseudomonas Sp.RT-1,TQ925
  16. Extraction, Purification and Antifungal Activity of Anthocyanins in Drop Fruits of Chinese Baberry,TQ914.1
  17. Preliminary Research on Characterization of Thermophilic Protease Pasey and Helicase Htc16,Q814
  18. Study on the Separation and Purification Way of Arctiin from Fructus Arctii and the Effect of Arctiin on the Growth of Mice C2C12 Cells,S853.7
  19. Purification of Human Respiratory Syncytial Virus Fusion Protein with Immunomagnetic Microsphere Technology,R373
  20. Identification of Marine Sreptomyces Sp. GY-4 and Researches on Its Antimicrobial Substances,Q936
  21. Prokaryotic Expression and Purification of Human Beta-Defensin-9,Q78

CLC: > Industrial Technology > Chemical Industry > Other chemical industries > Chemical processing industry of agricultural products > Chemical process and equipment > Chemical processing of agricultural products
© 2012 www.DissertationTopic.Net  Mobile