Dissertation > Excellent graduate degree dissertation topics show

Research of Abe Fermentation from Lignocellulose Using Recombinant Clostridium Acetobutylicum

Author: LiuChunYu
Tutor: HuangRiBo
School: Guangxi University
Course: Fermentation Engineering
Keywords: Clostridium acetobutylicum Cellulose Thiolase promoter Sugarcane bagasse ABE fermentation
CLC: TQ923
Type: Master's thesis
Year: 2013
Downloads: 26
Quote: 0
Read: Download Dissertation

Abstract


Clostridium acetobutylicum is a industrial strain that extensively used for production of biobutanol. Although this microorganism contains all the genes of cellulose-degrading enzymes, it cannot grow and ferment with cellulose as feedstocks.In this study, gene cphy3367was cloned from Clostridium phytofermentans genomic DNA by PCR. Recombinant E.coli-Clostridium acetobutylicum shuttle vector pSOS95-cphy3367was constructed and was successfully transformed into Escherichia coli. Results showed that the thiolase promoter initated the expression of gene cphy3367in E.coli by using real-time quantitative PCR and activity detection of recombinant enzyme. In conclusion, the thiolase promoter can be recognized by E.coli host cell.Recombinant plasmids were transformed into ATCC824by electrotransformation, thus recombinant strains ATCC824/pSOS95-cphy3367and ATCC824/pSOS95del were constructed. ABE (Acetone-Butanol-Ethanol) fermentation experiment with filter paper cellulose as the sole carbon source was carried out. Result:ATCC824could not utilizte cellulose as the sole carbon source; ATCC824/pSOS95del could produce ethanol. it means that the empty vector could affect the metabolic pathway in C.acetobutylicum and improve the ABE fermentation; ATCC824/pSOS95-cphy3367could yield acetone, ethanol and butanol with feedstock of cellulose, and the solvent production was higher than ATCC824/pSOS95del. Gene cphy3367enhanced the ability of fermentaion of C.acetobutylicum ATCC824with cellulose feedstocks.Furthermore, ABE fermentation experiment with hydrolyzate of sugarcane bagasse as the sole carbon source was conducted. Result:ATCC824could not ferment this materials to produce ethanol; ATCC824/pSOS95del could ferment and yield ethanol, and the yield of acetone and butanol was slightly lower than ATCC824; ATCC824/pSOS95-cphy3367could ferment and produce acetone, ethanol and butanol(0.78g/L,0.97g/L,1.09g/L, respectively), the yield of them and the total amount of solvent (2.84g/L) were higher than those of ATCC824and ATCC824/pSOS95del, these demonstrated that the recombinated strains with gene cphy3367have a enhanced ability of ABE fermentation with hydrolyzate of sugarcane bagasse as feedstock.

Related Dissertations

  1. The Environmental-Friendly Method and Process of Removing Non-Cellulosic Matters from Bamboo for Textile Fiber,TS652
  2. Sophorose the extraction and purification for the inter- type Neurospora cellulase produced induction of,TQ925
  3. Isolation of Highly Purified Cellulose from Wheat Straw and Its Application,X712
  4. The Preparation and Application of Anti-staining Agent on Denim Cellulose Washing,TS195.2
  5. Preparation of Low Molecular Weight Chitosan,TQ929.2
  6. Study on the Screening of Cellulolytic Strains Degradation Characteristic of Cellulose,TQ925
  7. Research on Preparation and Characterization of Mulberry Bark Sodium Carboxymethyl Cellulose,TQ261.1
  8. Shape Memory cellulose membrane preparation,TS195.55
  9. Microbial modification of plant fiber and its application in biodegradable composites,TB332
  10. The Preparation and Characterization of Functional Composite Humidity-Controlling Materials,TB34
  11. Research on new cellulose solvent system,TQ352
  12. Research on the Degradation of Straw Fiber for Chemical Derivatives Development,TQ352
  13. Prparation of Bacterial Cellulose with Jerusalem Artichoke,TQ352.1
  14. Preparation and Pharmacokinetics in the Beagle Dogs of PTU Sustained-release Tablets,R96
  15. Experimental Study of Biomass Pyrolysis Characteristics Based on Components Analysis,TK6
  16. The Research of Photocatalytic Degradation of Cellulose,TQ352.1
  17. Crop straw cellulose degrading bacteria Isolation and Screening,S216.2
  18. The cellulose fiber used as structural modification and dissolution behavior,TS102.1
  19. The Study of Pyrolysis Mechanism of Biomass,TK6
  20. Low oxygen alkali bagasse pulping system research,TS743
  21. Study on Gelatin/CMC Complex Coacervate Microencapsulation and New Crosslinking Agents,TQ460.1

CLC: > Industrial Technology > Chemical Industry > Other chemical industries > Fermentation industry > Fermentation legal system of higher alcohols and polyols
© 2012 www.DissertationTopic.Net  Mobile