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Fbrication of Graphene Functional Film and Patterned Stuctures for Cell Culture

Author: YangZhi
Tutor: LiuShaoQin
School: Harbin Institute of Technology
Course: Biomedical Engineering
Keywords: Graphene Layer-by-Layer self-assembly technique Nanocompositematerial Micro/nano processing Biocompatibility
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Type: Master's thesis
Year: 2012
Downloads: 7
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Abstract


As the basic structural unit of various allotropes of carbon,graphene hasattracted wide interest of scientists in the fields of science and engineering owing toexcellent electrical, optical, thermal, mechanical properties. In this paper, apatterned functional nanofilms was fabricated by LBL and nanoimprint techniquesusing graphene-based materials and co-cultured with HepG2cells to confirm thebiocompatibility of the nanofilms.The main achievements include:（1） PSS-stabilized graphene sol wassuccessfully prepared by Hummer’s method. PSS-stabilized graphene and PAH werethen self-assembled alternatively onto the ITO glass, slides, quartz and siliconsubstrates to constructed （PEI/PSS）2（PAH/PSS-G）100self-supporting multilayersnanocomposite films with layered structures by cross-section SEM images. Thenanocomposite film with average thickness and roughness of approximately90nmand5nm exhibited good mechanical strength and hardness, Young’s modulus andhardness were55GPa and2.5GPa respectively.（2） Novel UV light-aided catalytical synthesis method was developed toprepare graphene/polyaniline composites; and mophology of the gaphene/PANicomposites was regulated successfully via changing the concentration of anilinemonomers. When aniline comcentration wasn’t less than3mmol/L, nanorods withhundreds of nanometers in diameter,20m in length were obtained, otherwisenanoparticles with a diameter of about200nm1000nm were formed.（3） Based on miro/nano processing principles, patterns were fabricated in thefilms, and then biocompatibility was discussed through HUVEC cells toxicity testsin vitro and co-culturing with HepG2cells, which showed that the founctional filmwas no obvious toxic to HUVEC cells and was convenient for HepG2cells culturing.

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