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Study on the Culturing Technology of the Mini-juvenile-type Scion in Vitro of Hevea Brasiliensis Muell.Arg

Author: GuiMingChun
Tutor: DuanAnAn
School: Southwest Forestry University
Course: Tree Genetics and Breeding
Keywords: Hevea brasiliensis Muell. Arg. Mini-juvenile-typ scion Induction Subculture multiplication
CLC: S794.1
Type: Master's thesis
Year: 2012
Downloads: 1
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Hevea brasiliensisMuell. Arg.belongs to genus Hevea of Euphobiacea family.Originating in the tropocal rainforest of Amazon basin in Brazilian. It is an inportant sourseof natura tubber as its high yield, suprior quality and gather easily.Natural rubber plays asignificant role in the national economy.Graft clonal was the uppermost material in cultivation of Hevea brasiliensis Muell.Arg.on a large scale, but22months taked for breeding Graft clonal. In recently, a newbreeding technology what is rubber seed seedling bud grafting (rubber seed seedlings asthe rootstock, the mini-juvenile-type bud stick as the scion) has tended to mature. With thehelp of the technology, seed seedling bud grafting plantlet may be obtained in about half ayear and grow well after planted. The present mini-juvenile-type bud stick introduced fromChinese Academy of Tropical Agriculture Sciences was not suitable for Yunnan rubberplanting areas as its failure in anti-cold. So it’s in urgent to culture the mini-juvenile-typescion of Hevea brasiliensis Muell. Arg.in vitro which is suitable for Yunnan areas withhigh yield and anti-cold. In order to optimize the conditions and provide guidance oftheoretical and practical for cultivating the mini-juvenile-type scion of Hevea brasiliensisMuell. Arg. by tissue culture, this paper was to study on factors that effect the cultivationof mini-juvenile-type scion by comparison and orthogonal experimental design. The resultswere as follows:1. The optimum temperature for bud induction was at28/26℃(day and night), morethan1.50average of bud number, stronger shoots and less wilt. There was no obviousdifference in replicates. Subculture multiplication would well done at26℃and the rate ofnew shoots reached as high as23.4%.2. Different light intensities were needed for bud induction of different explants. Theinductive rate of base reached to95.3%at1600lx, with average bud of2.62. There weresignificantly difference in all treatments (p<0.01). The inductive rate of apical buds was93.30at1600lx. The inductive rate of stem was93.3%at800lx. There were significantlydifference in all treatments (p<0.05); and500lx was suitable for subculture multiplication,its propagation coefficient was1.62and the maximum elongation rate was31%.3.6-BA was closely related to bud induction, the bud number average was over2when the concentration of6-BA was1.0~2.0mg/L. The induction rate was more than94%and was obviously different with control (p <0.01).4. Adding2iP or ZT in2.0mg/L to the medium was good for bud induction. Theaverage of new shoots as low as1.54and2.18respectively, but they were strong and quick growing, the height averaged2.0cm after cultured for45d.5. NAA test showed that the buds could gain a maximum propagation coefficient(3.45) and a minimum mortality rate (6%) with adding0.2mg/L NAA to the medium.6. The orthogonal experiment results showed that: the best growth regulator was asfollow: for the stem bud induction was6-BA0.5mg/L+NAA0.2mg/L, the rate of newshoot reached to59%while the bud and elongation rate averaged2.33and53%respectively. For the bases was6-BA1.0mg/L+NAA0.2mg/L+KT0.5mg/L; For apicalbuds was6-BA2.0mg/L+NAA0.2mg/L(or0.4mg/L)+GA31.0mg/L; For subculturemultiplication was GA30.6mg/L only.7. The optimum induction for base bud was in medium of WPM, the average of budwas1.63, the rate of new shoot was85%. Stems in MS, the average of bud was1.00, therate of new shoot was60%. Apical in improved MS or WPM, the average of bud was1.25and1.14respectively, the rate of new shoot averaged in50%.

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CLC: > Agricultural Sciences > Forestry > Forest tree species > Special use of broad-leaved class > Rubber tree
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