Dissertation > Excellent graduate degree dissertation topics show

The Study of Ammonia-oxidizing Bacteria from Sediments in Bohai Sea

Author: TianFang
Tutor: DangHongYue
School: China University of Petroleum Hua Dong.
Course: Environmental Science and Engineering
Keywords: Bohai Sea sediment AOB amoA genes environment factor diversity
CLC: S917.1
Type: Master's thesis
Year: 2012
Downloads: 30
Quote: 0
Read: Download Dissertation

Abstract


Nitrogen is one of the basic elements for essential life activities. Various forms ofnitrogen exist in the biogeochemical cycling. Ocean nitrogen cycle plays an important role.Nitrification is a microbial facilitated process of the oxidation of ammonia to nitrate via nitrite.AmoA gene was widely used in the studies of Ammonia-Oxidizing Bacteria diversity. Tounderstand the composition and structure of Ammonia-Oxidizing Bacteria communities insedimentary environment of Bohai Sea, the diversity of amoA gene from the sediments ofBohai Sea was investigated by using a PCR-based gene cloning approach, combining thePrincipal Coordinates Analysis. A total of563amoA gene clones were obtained from6sampling stations. Restriction analysis of cloned amoA sequences obtained a total of49Operational Taxonomic Units (OTUs) from all samples. The number of OTUs in each stationwas ranged from15to18, which shows a low diversity of AOB in Bohai. The levels ofidentity of the amoA sequences to their best-match known sequences retrieved from theGenBank database varied from97%to100%. Molecular phylogenetic analysis of amoAsequences, being assigned into5Clusters(Clusters I-V), retrieved from the Gulf of Finland,estuary system, sediment of deep-sea、eurotrophic freshwater and coral reef and Jiaozhou Bay.The AOB are Nitrosomonas and Nitrosospira, the latter being the main AOB in B001、B005and B011station. Cluster Environment, Jackknife Cluster and Principal Coordinates Analysis(PCoA) indicated that the Ammonia-Oxidizing Bacteria communities of B001were unique.The Canonical Correspondence Analysis indicated that multi-environment factors, mainlysediment porewater concentration of NH4+-N controlled the community structure ofAmmonia-Oxidizing Bacteria.

Related Dissertations

  1. Chinese television media discourse paradigm of the evolution of the multi-angle analysis,G220
  2. Lake Sediment Accumulation Areas of Yunnan Altiplano Ecological Conditions of Phosphorus and Nitrogen Interactions,X131.2
  3. Survey and Analysis of Mycorrhizal Diversity in Zijin Mountain,S718.81
  4. Comparison of Genetic Diversity of Total Planktonic Bacteria and Active Planktonic Bacteria in Huguangyan Maar Lake in Summer,Q938
  5. Growth Pattern of Marsupenaeus Japonicus and Succesion of Plankton Community in Intensive Model,S968.22
  6. Numerical Study on Phosphorus Distribution in Water Environment of the Three Gorges Reservoir,X832
  7. Endophytic Fungal Diversity and Their Secodary Metabolites of Eleven Dominant Plant Species in the Dry-hot Valley of Yuanjiang, Yunnan Province, China,X172
  8. Shiraia its separation of fungal genetic diversity analysis,S567.39
  9. Genetic Diversity Analyses of Sillago Sihama Using AFLP Marker,S917.4
  10. Growth Rate and Growth Model of the F5 Selected Lines for Four Shell Colors of Pinctada Martensii （Dunker） Cultured in Deepwater Area of Beibu Bay in Zhanjiang and Their Genetic Diversity Analysis Using SSR Molecular Markers,S968.31
  11. Research on Optimization of the Relay Node Location of Cooperative Diversity,TN925
  12. ISSR Analysis of Genetic Diversity on 21 Lotus(Nelumbo Nucifera) Cultivars,S682.32
  13. SRAP and SSR molecular markers of pink flower Dendrobium genetic diversity and population genetic structure,S567.239
  14. The Research of Microsatellite Markers and the Eatablishment of Growth and Development Model in Xianan Cattle and Pinan Cattle,S823
  15. Red Soil Microbial Diversity of Peanut Continuous Cropping and Effects of Microorganisms on Growth of Continuous Cropping Peanut,S565.2
  16. Effect of Simulating Soil Environment on Soil Bacterial Diversity by PCR-RFLP Analysis,S154.3
  17. Characterization of Population Structure and Linkage Disequilibrium of Chinese Soybean Landerace Population and QTL Association Analysis of Traits Related to Breeding for Soybeans,S565.1
  18. Genetic Diversity, Genetic Variance Association Mapping of Fresh Seed Quality Traits QTLs of G.Max in China,S565.1
  19. Establishment of Transgenic Soybean, Maize and Wheat Informationg Platform and Effect of Transgenic Soybean on Soil Microorganisms,S565.1
  20. Numerical Simulation of Submarine Pipeline Self-buried Mechanism,TE973
  21. The Feature of Sediment Yield and Hydrodynamics of Different Underlying Surfaces in the Construction of the Shenfu-dongsheng Coal Field,S157.1

CLC: > Agricultural Sciences > Aquaculture, fisheries > Aquatic basic science > Aquatic Biology > Aquatic Microbiology
© 2012 www.DissertationTopic.Net  Mobile