Dissertation > Excellent graduate degree dissertation topics show

Zuogui hypoglycemic Yishen Decoction on mice with type 2 diabetic nephropathy podocyte structure and nephrin, podocin expression

Author: YinHongAi
Tutor: YuZuo
School: Hunan University of Traditional Chinese Medicine
Course: Chinese and Western medicine combined with clinical
Keywords: diabetic nephropathy MKR mice ZuoGui JiangTangYishen Decoction podocyte nephrin podocin
CLC: R285.5
Type: Master's thesis
Year: 2014
Downloads: 3
Quote: 0
Read: Download Dissertation


Objective To explore the possibility of progressive diabetic nephropathy in mice model by high fat diet feed and unilateral nephrectomy and investigate the effects of Zuogui Jiangtang Yishen decoction (ZGJTYS) on the structure of podocyte and expression of nephrin, podocin in podocyte of MKR mice with diabetic nephropathy (DN).Methods Firstly, establish the model of diabetic nephropathy:Sixty transgenic MKR mice (8weeks old) who expressed dominant-negative IGF-1receptor and insulin receptor in skeletal muscle were divided randomly into three groups as follows:ordinary feed group, high fat feed group, high-fat diet feed and mono-nephrectomy model group. The body weight, fasting blood glucose (FBG), micro albumin of urine (UmAlb) were detected at at1st,2nd,4th,6th,8th week. Eight weeks later, all mice were put to death, then blood and kidney were prepared to detect the serum BUN and Cr and observe the pathologic changes. Secondly, the part of drug intervention:Forty MKR mice (8weeks old) were divided randomly into four groups as follows:negative control group (group A), DN model group(group B), ZGJTYS group(group C) and positive control group(group D, Gliquidone and benazepri). All mice from group B, C and D were received high-fat diet feed and unilateral nephrectomy. Four weeks after operation, all mice were received drug intervention, and four weeks later, all mice were put to death. The levels of micro albumin of urine (UmAlb) were measured with double-antibody sandwich ELISA, the serum BUN and Cr with biochemical, and the fasting blood glucose (FBG) with electrochemical detection method. The morphological structure changes of the podocytes were observed with transmission electron microscopes. The nephrin and podocin mRNA expression were measured by RT-PCR, the protein expression by western blotting.Results The part of establishing the model of diabetic nephropathy:As compared with ordinary feed group, FBG in the mice of high fat feed group increased significantly. However, there was no difference in terms of FBG between high fat feed group and model group. BUN, Cr and urine UmAlb in the mice from model group increased significantly compared with ordinary feed group and high fat feed group (P<0.01). Compared with ordinary feed group, the kidney weight (KW) and the kidney/body weight (KW/BW)of mice in model group increased,and the renal pathological lesions of mice in model group were obvious compared with ordinary feed group; The part of drug intervention:As compared with group A, FBQ BUN, SCr and urine UmAlb in the mice of group B increased significantly (P<0.01), the expression level of nephrin and podocin mRNA and protein were markedly decreased(P< 0.01). After intervention of ZGJTYS, the renal pathological lesions of mice in group C and group D were significantly reduced compared with group B, all biochemical indicators above in the mice of group C and D significantly decreased (P<0.01), the expression level of nephrin and podocin mRNA and protein were markedly increased(P<0.01) compared with group B. However, there was no statistical significance in terms of all indicators above between group C and group D (P>0.05)Conclusion This model established by high-fat diet feed and mono-nephrectomy is ideal. ZuoGui JiangTang YiShen decoction exerts reno-protective effect via reinstating nephrin and podocin expression to repair the damaged podocyte.

Related Dissertations

  1. Low Dose of Spironolactone Reduces Oxidative Stress and Prevents the Development of Diabetic Nephropathy in Patients with Type 2 Diabetes Mellitus,R587.2
  2. Human adipose -derived mesenchymal stem cells to treat diabetic nephropathy rat studies,R692
  3. Effect of Angiopoietin-1 on Expression of TSP-1 and PEDF in the Kidney of Diabetic Rats,R692
  4. Herbal Drugs Attenuated DN by ERK and TGF-β-signaling Pathway,R277.5
  5. The Changes of T Lymphocyte Subsets and Costimulatory Molecules Before and After Shuxuetong Treatment in Type 2 Diabetes Subjects with Diabetic Kidney Disease,R692
  6. The Levels and Clinical Significance of Plasma ADMA and TGF-β1 in Patients with Type 2 Diabetic Nephropathy,R587.2
  7. The Study of Klotho Gene Expression Level in Debetics Nephometic Patients in Vivo,R692.9
  8. Correlation of type 2 diabetes serum TGF-β1 and PDGF-BB levels and urinary albumin excretion rate in patients with kidney disease,R587.2
  9. The Influence of Tripeterygium Wilfordii on Urine Podocyte Injury in Diabetic Kidney Disease Patients,R587.2
  10. Modified Zhulingtang treatment of diabetic nephropathy Ⅳ stage clinical study,R692.9
  11. The Significance and Level Changes of Urinary Albumin/creatinine Ratio and Serum Cystatin C on Type 2 Diabetic Nephropathy Patients,R587.2
  12. Effect of Gypenosides on the Expression of Nephrin、VEGF in the Kidney of Diabetic Nephropathy Rats,R692.9
  13. Knock-down of Cullin-5 Expression on Alteration of Morphology and Proliferation Ability in Glomerular Podocytes,R726.9
  14. The Curative Effect Comparison between Hemodialysis and Continuous Ambulatory Peritoneal Dialysis on End Stage Diabetic Nephropathy,R587.2
  15. Study on the Effects of the Mothod of Invigorating the Spleen and Kidney Detoxification and Vigoration of Blood Circulation on Early Diabetic Nephropathy and Flt-1 Expression on Rat Models,R259
  16. Dexamethasone on glomerular podocytes CD2AP/TRPC6 expression and distribution of,R726.9
  17. Children with nephrotic proteinuria and urinary podocyte nephrin related molecules detection,R726.9
  18. Investigate the Correlation between the Residual Renal Function and Cardiovascular Disease in Peritoneal Dialysis Patients,R54
  19. Kidney and spleen, Xiezhuo stasis treatment of chronic renal failure in clinical and experimental research,R277.5
  20. Rhubarb and Aconite different dose groups Asarum Decoction clinical observation of chronic renal failure and the impact of the of urinary podocytes flag protein PCX,R277.5
  21. Effect of YISHENTANG on Cell Cycle Regulatory Protein p27 in the Kidney of Diabetic Rats,R285.5

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
© 2012 www.DissertationTopic.Net  Mobile