Dissertation > Excellent graduate degree dissertation topics show

The Effect of Recombinant Hirudin on the Expression of S1P1Receptor in the Brain Tissue of EAE Mice Model

Author: TanQiang
Tutor: YuJuMing
School: North Sichuan Medical College
Course: Neurology
Keywords: Multiple sclerosis Experimental autoimmuneencephalomyelitis Recombinant hirudin S1P1
CLC: R744.51
Type: Master's thesis
Year: 2014
Downloads: 1
Quote: 0
Read: Download Dissertation


Objective: To investigate the effect of recombinant hirudin on theexpression of S1P1(sphingosine1-phosphate-1) receptor in the brain tissue ofmice with experimental autoimmune encephalomyelitis.Methods:64healthy female C57BL/6mice were randomly divided into3groups, respectively,22mice in the normal control group,22in EAE modelgroup and20in recombinant hirudin treated group.With water in oilemulsion antigen made of complete Freund’s adjuvant and MOG35-55,EAEmodel was established by pertussis toxin. On the fourteenth day HE stainingand LFB staining were used to assess EAE model. On the eighteenth day ofthe intervention group was given recombinant hirudin hypodermic (2.0mg/kg)injection, the normal group and the EAE model group were given the samedose of normal saline by hypodermic injection. Their appetite, mental stateand behavior change were observed daily; meanwhile their weight and neuralfunction defect were scored. Mice were sacrificed at the thirty-second day.The changes of S1P1receptor were observed with immunohistochemistrymethod; detection of S1P1mRNA expression level in brain tissue of mice wasdetected by RT-qPCR.Results:1.The normal group wasn’t found any onset of disease.The recombinanthirudin intervention group was significantly lower than the EAE groupof in nerve function defect score (P<0.05). The neural function defect score ofthe normal group and the recombinant hirudin group showed no statisticalsignificance (P>0.05). 2. Immunohistochemical staining showed expression of S1P1in braincells,in all groups.In normal group and the model group the expression ofS1P1receptor mainly located in the cell membrane, but in recombinanthirudin group in cytoplasm. Between the normal group and the EAE modelgroup, the average optical density difference showed no statisticalsignificance (P>0.05). Comparing with the average optical density either inthe normal group or in the EAE model group, the average optical density inthe recombinant hirudin group was significantly lower (P<0.05).3. RT-qPCR detecting showed the expression of S1P1mRNA in braintissue of mice in the recombinant hirudin group was significantly lower thanthat in the EAE group (P <0.05). S1P1mRNA expression in brain tissues ofmice in EAE group was slightly lower than that in the normal group (P>0.05)Conclusion:1. Recombinant hirudin ameliorates EAE mice.2. One of the therapeutic effects mechanisms of thrombin inhibitorhirudin may lies in inhibiting the expression S1P1receptor by intervening thecoagulation pathway.

Related Dissertations

  1. Chinese medicine intervention with multiple sclerosis correlation analysis of the T cell immune function,R277.7
  2. The Clinical and Basic Science Study of B7H1 Immunoregulation in Multiple Sclerosis,R744.51
  3. A Prospective Controlled Opening-Trial on Additive Treatment of Rebif in Chines Multiple Sclerosis,R744.51
  4. The Influence of Innate Immune Molecules and Cells in the Pathogenesis of Autoimmune Disease in Central Nervous System,R392
  5. Brain MRI brain tissue segmentation algorithm injury and multiple sclerosis,TP391.41
  6. The Clinical Application of 1H-MR Spectrocopy in Multiple Sclerosis,R744.51
  7. Establishment of Animal Model for Experimental Autoimmune Encephalomyelitis and Matrix Metalloproteinase-9 Expression on EAE Rats,R-332
  8. Curative Effect Observation of Treating Multiple Sclerosis with Simvastatin and Corticosteroid and Its Effect on Serum Levels of IL-6,R744.51
  9. Antibody to Aquaporin 4 in the Differential Diag-nosis of Multiple Sclerosis and Neuromyelitis Optica,R744.51
  10. Cell-based Assay and Related Titre Test for Aquaporin-4 Antibodies in Neuromyelitis Optica of Chinese Populations,R744.52
  11. The Value of Cerebrospinal Fluid IgG Index and Oligoclonal Bands in the Diagnosis of Multiple Sclerosis,R744.51
  12. The Research on the Association of VDR Gene ApaⅠ and BsmⅠ Polymorphisms with Multiple Sclerosis,R744.51
  13. Prkaryotic Expression’s System Construction of Interleukin-17A to Mice,R392
  14. Amelioration of Experimental Autoimmune Encephalomyelitis Brain and Expression of BCL-2、FAS in Rats by FTY720,R965
  15. The Clinical Characteristics of 105 Patients with Multiple Sclerosis and Treatment Analysis,R744.51
  16. The Research of Determination of Serum KLK1 and KLK6 Levels of Patients with Multiple Sclerosis,R744.51
  17. High-abundant Protein Depletion Method and Complement Related Biomarkers Research of CSF from MS Patients,R744.51
  18. Effects of Atorvastatin on Expression of NF-kB and GFAP in Rat with Experimental Autoimmune Encephalomyelitis,R965
  19. The Brain Diffusion Tensor Imaging and Pathogenesis of Cognitive Impairment in Multiple Sclerosis,R744.51
  20. The Mechanism of the Rho/Rock Signal Pathway, CD8~+T Cells and Fasudil in Experimental Autoimmune Encephalomyelitis,R744
  21. The Impairment of Information Procession Speed and Working Memory on Multiple Sclerosis,R744.51

CLC: > Medicine, health > Neurology and psychiatry > Neurology > Spinal cord disease > Demyelinating disease > Multiple sclerosis
© 2012 www.DissertationTopic.Net  Mobile