Dissertation > Excellent graduate degree dissertation topics show

The Clinical Significance of Aberrant Expression of Twist and E-cadherin Proteins in Colorectal Carcinoma

Author: WangYuan
Tutor: GaoZhiAn
School: Liaoning Medical
Course: Pathology and Pathophysiology
Keywords: Immunohistochemistry Twist E-cadherin Colorectal carcinoma Westernblot
CLC: R735.34
Type: Master's thesis
Year: 2013
Downloads: 0
Quote: 0
Read: Download Dissertation

Abstract


ObjectiveDetecting the expression of Twist and E-cadherin proteins, to investigatethe role of aberrant expression of the two proteins in colorectal carcinoma.MethodsImmunohistochemistry(IHC) method was used to detect the expression ofTwist and E-cadherin proteins in78cases of colorectal carcinoma,42cases ofadenoma and20cases of paracancerous mucosa tissues; Twist proteinexpression level was detected by Western blot method in30cases ofcolorectal carcinoma and30cases of adenoma.ResultsIHC results:①the positive rates of Twist protein were56.4%(44/78),26.2%(11/42) and10%(2/20), while the positive rates of E-cadherin proteinwere48.7%(38/78),76.2%(32/42) and100%(20/20) respectively in colorectalcarcinoma, adenoma and paracancerous mucosa. The significantdifferences statistically were found between the groups of paracancerousmucosa,adenoma and carcinoma;②Lower positive rates of Twist proteinwere observed in the groups of well-moderate differentiation(40%) andnegative lymph node metastasis(43.2%) than that in the groups of poorlydifferentiation(78.8%) and positive lymph node metastasis(73.5%), significantdifferences statistically were found between the groups. But no significantdifferences was found between the groups of the degree of infiltration; Higherpositive rates of E-cadherin protein were observed in the groups ofwell-moderate differentiation (62.2%), non-lymph node metastasis (59.1%) and early infiltration (74.1%) than that in the groups of poorlydifferentiation(30.3%), lymph node metastasis (35.3%)and advancedinfiltration(35.3%);③The negative correlation between the expression ofTwist and E-cadherin proteins was obtained in colorectal carcinoma)r=-0.488,p=0.000).Western blot results: Higher expression quantities of Twist protein wasobtained in the group of colorectal carcinoma than that in the groups ofadenoma and paracarcinoma mucosa; In the groups of well-moderatedifferentiation and non lymph node metastasis, lower positive rates of Twistprotein was observed than that in the groups of poorly differentiation andlymph node metastasis, significant differences statistically were foundbetween the two groups, but no significant differences was observed betweenthe groups of the degrees of infiltration.ConclusionsIt was suggested that over-expression of Twist protein and low-expressionof E-cadherin protein might play an important role in pathogenesis andprogression of colorectal adenocarcinoma.

Related Dissertations

  1. Research on BT Toxin Oligomer Formation Induced by Lipid Raft and the Immunodetection Explore of Cadherin,S476.1
  2. Fitness Costs of a Disrupted Cadherin Allele Conferring Cry1Ac Resistance of Helicoverpa Armigera,S435.622.3
  3. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  4. The Study of Kisspeptin/GPR54 System on the Reproductive Regulation in the Hen’s Hypothalamus-Pituitary-Ovary Axis,S831
  5. Study on the Digestive Tube During the Embryonic Stage of Gushi Chicken,S831
  6. A Comparative Study of prawns, crabs, Spiroplasma disease immunohistochemistry and pathology,S945
  7. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  8. Expressions of PRL-3 and p27 in Gastric Carcinoma and Their Clinical Significance,R735.2
  9. Expression of hnRNP A2/B1 and p21WAF1 Proteins in Lung Cancer and Their Clinical Significance,R734.2
  10. The Effects of RIP on the Immune Function of Renewal Rats Without Colostrums,S853.7
  11. Study on the Expression and Correlation of Metallothionein and Matrix Metalloproteinase-2 in Non-small Cell Lung Cancer,R734.2
  12. Expression and Their Significance of Apoptosis Regulatory Factors Survivin、XIAP and c-Jun、NF-κB in Epithelial Neoplasm of Ovary,R737.31
  13. Expression and Clinical Significance of Survivin and YAP in Cervical Carcinoma,R737.33
  14. Expression and Clinical Significance of PTEN and Caspase-7 in Gastric Cancer and Precancerous Lesion,R735.2
  15. Cloning and Expression Analysis of NCCRP-1 AND IL-10 Genes in Grass Carp (Ctenopharyngodon Idellus),S917.4
  16. Expression and Antibody Preparation of sglt1 from Cyprinus CarpioL.,S917.4
  17. The Effect of Hepatic Denervation on Liver Regeneration after Partially Hepatectomy in Rats,R363
  18. Expression and Correlation of Caspase-3 and Cx43 in Myocardial Infarction with Different Narrow Degree Coronary Artery,R542.22
  19. The Expression and Relationships of Bmi-1 and c-myc in Diffuse Large B-cell Lymphoma Tissues,R733.1
  20. Expression of Caspase-4 and Caspase-3 Gene Related Apoptosis in Pathological Changes of Sternocleidomastoid of Congenital Muscular Torticollis and Four Years Clinical Followed-up for Opereated Patients,R726.8
  21. The Feasibility of the Immunohistochemistry Classification to Predicting Prognosis in Breast Cancer,R737.9

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Intestinal neoplasms > Colorectal tumors
© 2012 www.DissertationTopic.Net  Mobile