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Objective: To detect the block strains (Kunming) mouse secondary oocyte gene expression differences in the levels of the mice with non-blocking strain (B6C3F1); observed fibroblast growth factor (fibroblast growth factor, FGF7) on the KM small mouse implantation the former embryo development and to overcome the 2 - cell block. Methods: 1.Affymetrix-expression microarray detection KM and B6C3F1 mice secondary oocyte gene expression differences, and the use of Real time PCR to verify the part of the differentially expressed genes; 2.RT-PCR and immunofluorescence cytochemistry chromogenic detection fibroblast growth factor receptor (fibroblast growth factor receptor, FGFR) -1,2 and 3 KM mouse oocytes and implanted before the embryo in the expression and distribution; 3. collect KM mice - embryos The droplet culture method continuous culture was observed in M16 medium supplemented with of FGF7 protein in the pre-implantation embryo development, to observe whether overcome the 2 - cell block. Results: 1. Microarray gene expression associated with gene transcription, transcription regulation, protein synthesis and other functions within the KM and B6C3F1 mouse oocytes differences; Real time PCR validation results with the chip test results more consistent, indicating that this sub-chip test results more reliable. 2.RT-PCR detection display, FGFR1, FGFR2 and FGFR3 mRNA expression in the KM mouse oocytes and pre-implantation embryo; immunofluorescence cytochemistry combined with confocal scanning microscopy showed FGFR1, FGFR3 immunoreactive reaction seen in oocytes cells and the periphery of the pre-implantation embryo cytoplasm near the cell membrane; the FGFR2 positive reaction uniformly distributed in the cytoplasm of the oocyte and pre-implantation embryo. 3.Km mouse - embryos cultured in M16 culture medium Add FGF7 protein, 4 - cell embryo development was significantly higher than the blank M16 culture medium (control group), the difference was significant. Conclusion: B6C3F1 mouse oocytes regulated genes regulate gene transcription, oxidative stress, protein synthesis and transport, amino acid phosphorylation, cell cycle, development, suggesting B6C3F1 mouse oocytes with more comprehensive regulation of gene transcription, protein synthesis and transport, antioxidant, blocking and non-blocking strains of mice oocytes maternal gene expression differences may affect mouse preimplantation embryos in capacity; KM mouse oocytes and pre-implantation embryo fibroblasts growth factor receptor 1, 2, and 3 expression; FGF7 promote KM mouse pre-implantation embryo development in vitro, significantly increased 2 - cells to 4 - cell development ratio.
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