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Effects and Mechanism of Epigallocatechin-3-gallate on Alcohol Absorption in Mice with Acute Alcoholism

Author: WangLing
Tutor: JiangLing；WeiWei
School: Anhui Medical University,
Course: Pharmacology
Keywords: Table epigallocatechin gallate Acute alcohol intoxication Locomotor activity Morris Water maze Transfer rod experiment Alcohol dehydrogenase P-gp
CLC: R285.5
Type: Master's thesis
Year: 2010
Downloads: 136
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Abstract

Tea is rich in polyphenols, purine alkaloids, vitamins and amino acid in ancient Chinese medical literature multiple records tea drug hangover. Studies have shown that tea extract has a hangover effect. Table epigallocatechin gallate (epigallocatechin-3-gallate, EGCG) is the main one of the pharmacologically active ingredient of tea polyphenols, but the role of EGCG on acute alcoholism has not been reported. In this study, 56% (v / v) Erguotou 5g/kg IG. Mice to establish the Acute Alcoholism model, observation of EGCG on acute alcoholism mice neurobehavioral indices (locomotor activity test, turn rod test, Morris water maze) blood alcohol concentration and detection of liver and stomach alcohol dehydrogenase (alcohol dehydrogenase, ADH) activity and liver and ileum of P-gp expression to explore the mechanism of EGCG on ethanol absorption may reveal a new mechanism of ethanol absorption to provide a new basis. Objective: To establish a mouse model of acute alcoholism, and to explore the EGCG neurobehavioral markers of acute alcohol intoxication in mice, blood alcohol concentration and its possible mechanism of action. Methods: The mice were randomly divided into normal group, model group, group EGCG 25mg/kg, EGCG 75 mg / kg group, EGCG 225 mg / kg group, Pueraria capsule (180 mg / kg), normal group and model group were normal saline administered group ig given different concentrations of drugs 0.5h, model group and the administration group 5g/kg IG given 56% (v / v) Erguotou the establishment of acute alcoholism model in mice, locomotor activity, Morris water maze, the transfer rod test inspection EGCG in mice of acute alcoholism; headspace gas chromatography assay blood alcohol concentration; ultraviolet spectrophotometry to determine liver homogenates and the protein concentration in the stomach homogenates and alcohol dehydrogenase activity; Western blot assay liver and ileum content of P-gp expression. Results: Compared with normal group, model group mice locomotor activity was significantly increased compared with model group, EGCG (75,225 mg / kg group) lower in the 0.25 to 1h when locomotor activity, which EGCG 225 mg / kg 1h group, locomotor activity was significantly lower (P lt; 0.01). EGCG 225 mg / kg group and Pueraria capsule group were significantly alleviated ethanol-induced mice spatial discrimination barriers (P lt; 0.01) EGCG (75,225 mg / kg group) and Pueraria capsules in 0.75 ~ 2h, can significantly ease the ethanol-induced mouse imbalance phenomenon, the difference was significant (P lt; 0.01) 2. Compared with model group, EGCG (75,225 mg / kg group) when the blood alcohol concentration of 0.5-1h lower, the difference was significant (P lt; 0.05), especially EGCG 225 mg / kg group blood alcohol concentration in the first hour when compared with the model group, the difference was significant (P lt; 0.01). Compared with normal group, model group mouse liver and stomach ADH activity decreased, the difference was significant (P lt; 0.05), 0.25h to 2h when EGCG 75mg/kg, 225 mg / kg group liver ADH activity was significantly enhanced, especially EGCG 225 mg / kg liver ADH activity was significantly increased (P lt; 0.01), EGCG 75 mg / kg and 225 mg / kg group of mice in 0.25h to 2h stomach ADH activity significantly enhanced, The difference was significant (P lt; 0.01), prompted EGCG may be provided by the enhancement of the liver and stomach ADH activity, improve the degree of decomposition of ethanol, to reduce the role of the concentration of alcohol in blood. (3) Compared with normal group, model group mice liver and ileum in P-gp of expression increased, the difference was significant. Compared with model group, 1h P-gp expression in the mouse liver of EGCG three dose groups decreased, the difference was significant, P-of 1h when EGCG 25 mg / kg group and 75 mg / kg group of mice ileum gp expression decreased, the difference was significant (P lt; 0.01), 2h when EGCG 25 mg / kg group and 75 mg / kg of mouse liver and ileum in P-gp the reduced expression of the difference was significant. Suggesting that ethanol may have a role to promote liver and ileum of P-gp expression, EGCG may inhibit liver and ileum of P-gp expression. Conclusion: EGCG may have the role of inhibition of ethanol-induced increased excitability of the central nervous system of mice, to improve the ethanol-induced spatial discrimination in mice disorder and imbalance; EGCG affect ethanol absorption mechanism may be enhanced liver and stomach in ADH activity, enhanced first-pass metabolism of ethanol, the ethanol into the blood, lower blood alcohol concentration, improve the neurological behavioral symptoms of acute alcoholism; ethanol may have enhanced the expression of P-gp in the liver and ileum role, and EGCG may inhibit P-gp expression in the liver and ileum role.

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