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The Effect of ET-1 on the Renal Tubular Epithelial-mesenchymal Transition in DN Rats and the Impact of Cordyceps Sinensis Thereon

Author: XiaoZhou
Tutor: ZhouQiaoLing
School: Central South University
Course: Internal Medicine
Keywords: Diabetic nephropathy Tubular epithelial transdifferentiation Endothelin Cordyceps
CLC: R285.5
Type: Master's thesis
Year: 2010
Downloads: 108
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Abstract


Objective To observe the high glucose ET-1 expression levels in kidney tissue and renal tubular epithelial transferred differentiation-related protein α-SMA and E-cadherin expression status and Cordyceps dope intervention experiments to observe the changes in the protein expression, and to further explore diabetes nephropathy pathogenesis of renal fibrosis, and Cordyceps sinensis on the mechanism of the protective effect of this pathological process. Methods 10 Wistar rats by intraperitoneal injection of streptozotocin (STZ) (STZ, 60mg/kg), induction into the DM model measured 24-hour urinary protein excretion DM model success after four weeks, including a 24-hour urine protein greater than 30mg that the DN successful model, were randomly divided into the model group, CS group, n = 5. Another five mice aged rats weight match as a normal group. The Cordyceps groups to Cordyceps powder suspension 1.0g / (kg * d), and the control group with normal saline orally. To intervene experiment rats were sacrificed four weeks after each group of five. Measured prior to sacrifice 24-hour urinary protein excretion, were killed after leaving blood specimens measured serum creatinine; renal tissue, HE and MASSON staining to observe the morphological changes of kidney tissue; immunohistochemical methods to detect renal tissue ET-1, α-SMA using RT-PCR method to detect ET-1, α-SMA and E-Cadherin mRNA expression and protein expression of E-Cadherin; Results 1 diabetic nephropathy organizations, ET-1 generates increased α-SMA expression rates decreased expression of E-cadherin (p lt; 0.05 or p lt; 0.01). ET-1 and α-SMA was positively correlated with E-Cadherin was negatively correlated (p lt; 0.01), prompted the ET-1 induced tubular epithelial transdifferentiation promote renal interstitial fibrosis (p lt; 0.01). model group rats 24-hour urine protein, serum creatinine levels, glomerular hypertrophy and renal tissue, inflammatory cell infiltration, extracellular matrix increased renal interstitial fibrosis and pathological changes. (P lt; 0.05 or p lt; 0.01) 3. The Cordyceps intervention in rats with diabetic nephropathy rats significantly reduced urinary protein excretion, elevated serum creatinine delay alleviate diabetic nephropathy renal pathology change (p lt; 0.05 or p lt; 0.01); kidney tissue, reduce the secretion of ET-1, α-SMA downregulated expression of E-cadherin raised (P lt; 0.05 or P lt; 0.01). Conclusion 1.ET-1 induced tubular epithelial transdifferentiation and promote the role of renal fibrosis in diabetic nephropathy. . Cordyceps has some renoprotective effect on diabetic nephropathy and anti-glomerular fibrosis and its mechanism of action may be to inhibit ET-1 secretion leaving the downregulation of α-SMA, E-cadherin upregulation.

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