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Studies on the Active Constituents of Swertia Nervosa(G.Don) Wall. and Content Determination of Two Bitter Secoiridoid Glycosides

Author: LiChangXi
Tutor: TanGuiShan
School: Central South University
Course: Medicinal Chemistry
Keywords: Selaginella Cushion Selaginella selaginellin Amentotaxus Biflavone Determination
CLC: R284
Type: Master's thesis
Year: 2010
Downloads: 75
Quote: 1
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Abstract


Macroporous resin column chromatography , polyamide column chromatography , silica gel column chromatography, preparative chromatography and other modern technologies, from cushion isolated Selaginella prepared selaginellin (1) and amentoflavone (2) The two characteristic ingredients, and the use of UV, IR, NMR ( including 2D-NMR) and MS ( including HRMS) and other modern spectroscopic techniques identified its structure , HPLC-DAD normalization method to detect the purity of greater than 99.0% . For the first time established a rapid quantitative analysis of HPLC-DAD method - the simultaneous determination of two types of herbs Selaginella characteristic ingredients : a new phenolic compounds selaginellin acetylene and typical biflavonoids amentoflavone, and herbs Selaginella batches GB and cushion as well as non GB Selaginella Selaginella and Jiangnan Selaginella prostrate herbs were determined. System study methodology and different varieties of assay results show that the method is simple, rapid , accurate, sensitive , reproducible, and amentoflavone to selaginellin good controllability for the quality control indicators , specific, and has a strong identification significance Selaginella herbs for quality evaluation and control reference.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical
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