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Application of nanobiotechnology , immunological techniques and modern cell biology research techniques to prepare magnetic nanoparticles and immune nano fluorescent microspheres ( Immunologic the the Nano - Fluorescence beads , INFB ) . The application of magnetic nanoparticles , we can be an effective separation of target cells , immune nano fluorescent microspheres (INFB ) can detect the umbilical cord blood stem cell surface antigen expression . Isolated from umbilical cord blood mononuclear cells ( MNC ) MNC culture , cryopreservation, amplification and differentiation , immune nano fluorescent microspheres , streaming detection hematopoietic stem and progenitor cells \empty \The study found that (1) nano fluorescent microspheres can be used for identification of target cells in vitro fluorescence spectrometry , magnetic nano-particles can effectively isolated from the target cell , INFB cells without biological toxicity . (2) With the prolonged incubation time , the proportion of mononuclear cells in CD34 and CDll7 cells gradually decreased ; CD34 CDl33 competitive binding characteristics of the cell surface antigen . ( 3 ) hematoxylin large Sargentodoxa , Qin Brazil mushroom water extracts and tea polysaccharide induced cord blood mononuclear cells , CD34 , CD33 , CD14, CD119 , CD19 , CD4 positive cells value and more Chinese medicine dose-dependent relationship . DC and NK cells ( 4 ) Chinese medicine polysaccharides Astragalus can effectively induce DC cells and NK cells , and induced biological activity .
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