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Research on Preparation of Magnetic Silica Composite Nanoparticles for Immunochromatography Assay

Author: ChenChun
Tutor: YangHai
School: Huazhong University of Science and Technology
Course: Biopharmaceutical engineering
Keywords: silica reverse microemulsion method Stober method magnetic nanoparticles preparation magnetic immunochromatography assay
CLC: TQ127.2
Type: Master's thesis
Year: 2013
Downloads: 12
Quote: 0
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Abstract


Lateral flow immunoassays (LFIAs) is a simple and rapid on-site detectiontechnology, and has been widely used in the field of biomedical testing.Immunochromatography assay labeled by colloidal gold has already realized large-scalecommercial applications in clinical diagnosis, epidemiological screening, environmentaland food monitoring. Colloidal gold labeled immunochromatography technology is amethod based on optical analysis, only signals on the surface of chromatographymembrane can be detected. Signals below the surface of chromatography membrane cannot be measured, and therefore this method based on colloidal gold can not achieve truequantitative analysis.Superparamagnetic nanoparticles have been widely used in biomedical applications.Detection can not be quantified while labeling with colloidal gold. Since the magneticsignals of superparamagnetic nanoparticles can penetrate chromatography membrane,these defects can be overcome. It has advantages of high sensitivity, low interference andquantitative detection while labeling with superparamagnetic nanoparticles. At present,researches on applications of superparamagnetic nanoparticles in immunoassay weremainly focused on magnetic separation immunoassay technology, but relatively fewstudies were involved in magnetic immunochromatography assay.Large size magnetic particles were required in the magnetic separation. However,magnetic composite nanoparticles for immunochromatography assay should have smallerand uniform size (100~300nm), stable dispersion, magnetic response performance and beeasy coupling with biological molecules. These are also the problems needed to be solvedurgently at present. Currently there are no still commercial magnetic compositenanoparticles for magnetic immunochromatography. So the purpose of this research is tooptimize a preparation method of smaller size magnetic composite nanoparticles forimmunochromatography.In this study, the Stober sol-gel method and reverse microemulsion method were usedto optimize the preparation conditions of silica nanoparticles. Influencing factors on silicananoparticles preparation in microemulsion were then investigated to determine an appropriate microemulsion system. Conditions in reverse microemulsion for preparationof core-shell structure magnetic silica nanoparticles were also optimized. The compositeparticle size and size distribution were characterized through the DLS and TEM, and thencompared it with Stober sol-gel method. Finally, immunochromatography experimentswere carried out to validate the feasibility of magnetic silica nanoparticles as labeledparticles for immunochromatography quantitative detection. The experimental resultsshowed that magnetic silica nanoparticles with certain magnetic response prepared by thereverse microemulsion method were about100nm in mean size with high package rate.Through the research on preparation, it was found that it was very important when themagnetic nanoparticles should be added into the microemulsion system. It showed thatmagnetic nanoparticles may be easily combined with primary silica particles (<5nm)afterTEOS hydrolysis. Magnetic immunochromatography results showed that amino modifiedmagnetic silica nanoparticles had immune competition reaction, which was promising inimmunochromatography application as signal material. These studies also provide a newideas for coating magnetic nanoparticles with inorganic material used in immunoassay.

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CLC: > Industrial Technology > Chemical Industry > Non-metallic elements and their inorganic compounds, the chemical industry > Part Ⅳ family of non-metallic element and its inorganic compounds > Silicon and its inorganic compounds
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