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Capillary electrophoresis technology developed since the 1960s, has gradually become an important means of analytical separation detection, this paper is divided into five chapters to introduce the application of capillary electrophoresis in the detection of the enzyme-linked immunoassay and natural products active ingredients. The first chapter the basic principles of capillary electrophoresis (CE) separation mode detection technology introduction, cited capillary technology progress and prospect; introduced by capillary electrophoresis with electrochemical detection. Capillary electrophoresis electrochemical immunoassay analysis of carbohydrate. Topic and be brief description. Chapter II on the basis of capillary electrophoresis - electrochemical detection research, the application OPD-H 2 O 2 -HRP indirect determination of HRP content of the system put forward a series means improvement measures. Combined with a simple and reliable detection interface, to solve the problem of conventional platinum microelectrode capillary docking consuming, inaccurate docking; pool into the sample pool with a buffer solution made of airtight plexiglass instrument, to improve experimental weathering and anti-interference ability. Increase the height of the sample cell, and to solve the problem of the height difference between the sample cell with the substrate; to using homemade polyvinyl alcohol coated pipe, cost savings, and to improve the detection results. Chapter capillary zone electrophoresis with electrochemical detection (CZE-ED) were detected and isolated D-galactose, lactose, sucrose, glucose, arabinose. Use of cyclic voltammetry to five sugar standard was detected potential of 0.7V; ScS electrophoresis buffer concentration, separation voltage and the injection time of the D-galactose, lactose, glucose, sucrose, arabinose electrophoresis migration behavior, to finalize the copper disk electrode as the working electrode, the working electrode potential of 0.7V (vs. saturated calomel electrode), the electrophoresis medium of 0.1 mol L -1 NaOH, separation voltage the injection voltage is 15kV, 15kV, the injection time of 10s. Under this condition, the five sugar better able to detect and separation. Chapter by water extraction and alcohol precipitation extraction and purification of Angelica and Codonopsis polysaccharide, the dried polysaccharide extracted with 1 mol · L -1 sulfuric acid hydrolysis in the 100 ° C water bath. Subsequently, the establishment of a rapid determination of Angelica Polysaccharide monosaccharide composition of high performance capillary electrophoresis electrochemical methods. In the second chapter of the optimized conditions, the migration time of the five sugar mixed samples were investigated and determined the monosaccharide composition of Angelica Polysaccharide. Three sugars Angelica hydrolysis samples detected by the standard addition method, and the migration time in the electrophoresis sample spectra for comparison with five sugar of the standard mixed, identified by three monosaccharide is lactose, sucrose, arabinose; Codonopsis hydrolysis the three sugars detected in the sample by the standard addition method, and the migration time in the electrophoresis sample spectra for comparison with five sugar of the standard mixed, identified by three monosaccharide is lactose, D-galactose, sucrose. The establishment of a rapid detection method of Chinese herbal medicine in the sugar component content. Chapter capillary electrophoresis electrochemical method for separation and determination of Cnidium and its preparations coumarin active component. ScS background electrolyte pH buffer solution, the concentration of the surfactant, the kind and concentration of the organic modifier on the separation system. Experimental results show that: at a buffer concentration of 10mmol · L -1 buffer pH 10.5, SDS concentration of 20 mmol · L . -1 , a methanol concentration of 10% when the optimized conditions, Cnidium and its preparations coumarin active component of the baseline separation, and the method has good reproducibility.
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