|
In this study, the mitochondrial DNA of the cytochrome b gene sequences surrounding areas in Guizhou six goat breeds 72 individuals cytochrome b gene was amplified by PCR , sequencing , analysis, we get the following conclusions: 1. Guizhou surrounding area six goat breeds 72 individual mtDNA Cyt b gene sequences length is 1140bp, are based on the start codon ATG start , with no insertions / deletions to the end of the stop codon AGA . A T the average content of 58.7%, G C average content of 41.3% . 2 Guizhou vicinity six goat breeds 72 individual mtDNA Cyt b gene haplotypes have 18 kinds , produced a total of more than 30 polymorphic sites , of which two single nucleotide polymorphic loci 17 , two bases variation between parsimony informative sites for the 13 ; base substitutions in 30 loci observed 74 non-synonymous mutations occur at codon 66 of the first one, two occurred in the second codon bit , six times in the codon first and second bits are mutated . 3 six goat breeds haplotype diversity was 0.505-0.808 , nucleotide diversity was 0.025% ~ 0.246% , both in the mid to upper level, indicating that Chinese goat breeds rich diversity . 4 According to mtDNA Cyt b gene haplotypes 18 kinds of phylogenetic analysis , the observed three branches , namely branches A, B, and lineage lineage C, the frequency of occurrence were: branch A93.06% ( 67 / 72 ) , branch B5.55% (4/72) and lineage C1.39% (1/72), showed that six goat breeds ( groups ) in the vast majority of individuals that originated in a matrilineal lineages A. 5 through six goats mitochondrial DNA cytochrome b gene analysis system origin , indicating that six goats were originated in Southwest Asia bezoar goat (Capra aegagrus). 6 This study found that different genetic relationship among cultivars and varieties Dungeon distribution are closely linked , the more geographically close geographical relationships between the more recent , and vice versa away.
|