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Selection and RAPD Identification of Excellent Auricularia Auricula Strains by Ultraviolet Mutation

Author: ZhaoLi
Tutor: ChenYanQiu
School: Yanbian University
Course: Pomology
Keywords: Auricularia auricula mycelium ultraviolet radiation RAPD
CLC: S646.6
Type: Master's thesis
Year: 2009
Downloads: 49
Quote: 1
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Abstract


Auricularia auricula belong to the Basidiomycotina,Auriculariales, Auricul -ariaceaeAuricularia.It abundance nutrition,odorc delicious,not only the taste mushroom but lnly the officinal mushroom,It is reciqnized health care leechdom all over the world. Auricularia auricula also have the importent economy value fungs,it plant in china for hundred years.Along with Auricularia auricula manufacture development,many fungs reaschers have already seclected many choiceness,But it is not suit for present manufacture,So selected higher production and choiceness fungs is very important task.this experimen adopt ultraviolet radiation the mycelium seqment for different time,the result showed that:The ultraviolet radiation the strain named 00 for lmin and 2min,mother mycelium growth speed is 4.92 mm·d-1 and 4.94 mm·d-1, mother mycelium weight is 0.147 g·25mL-1 and 0.171 g·25mL-1,original mycelium growth speed is 5.80 mm·d-1 and 5.46 mm·d-1, all of them achieve max and markedness excelled orther treatment. The treatment witch Radiation 1min and 2min from inolulation to mushroom form of the calticuture stains ahead 10 days and 11 days, also the fruit body colour is black,the roots is small and the thick of fruit body is 0.54cm and 0.67cm,it have the markedness excelled with orther treatment. So the auricularia auricula strains named 00 through ultraviolet radiation for lmin and 2min is choiceness and aberrance strains,and they are named U01 and U02.The auricularia auricula strains named 01 through the ultraviolet radiation for1min and 2min,the morther mylucim and orignal mylucim growth speed, morther mylucim weight all have the markedness excelled to other treatment, morther mylucim growth speed is 4.66 mm·d-1 and 4.15 mm·d-1, morther mylucim weight is 0.187 g·25mL-1、0.193 g·25mL-1, orignal mylucim growth speed is 5.29 mm·d-1 and 5.34 mm·d-1;when the strains radiation forlmin and 2min caltivation strains the time from inolulate to fruit body form ahead seven days and eight days,and the position where the group form is centraliza the blade,single ear average weight is 1.41g and 1.87g,the biology efficiency is 6.25% and 6.56%,all of this markedness excelled orther treatments. So the auricularia auricula strains named 01 through ultraviolet radiation for lmin and 2min is choiceness and aberrance strains,and they are named U11 and U12. 3、To molecule marked the orginal strains and irratiation strains through the RAPD technology ,the results showed that the gene distance frome orginal strains to irratiation strains between 0.2000~0.7258, gene distance very far so it can determinant U01,U02,U11,U12 is mutagenic strains.

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CLC: > Agricultural Sciences > Gardening > Vegetable gardening > Fungi ( mushroom ) > Fungus ( cloud ear fungus,black fungus )
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