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Effect of Exogenous L-carnitine on Activities of Respiratory Chain Complexes in Mitochondria and Free Radicals Metabolism from Mice’s Heart

Author: YuXiaoYan
Tutor: LiJie
School: Northwest Normal University
Course: Human Movement Science
Keywords: L-carnitine heart mitochondria respiratory chain activity of enzymatic complexes free radicals metabolism
CLC: G804.2
Type: Master's thesis
Year: 2009
Downloads: 94
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Abstract

L-carnitine as the only transport for long-chain fatty acid in the mitochondria membrane,which plays an important role in the process of energy metabolism.By means of perfusing L-carnitine into mice’s stomach and exercise training ,applied theory and method of sports athletic physiology and cell biology to find the differences of mitochondrial enzyme complex’s activity and oxygen free radicals content in the rats’heart muscle,to examine the effect of supplying L-carnitine and training on activities of respiratory complexes and try to find a way to improve mitochondrial function by nutrition supplement.methods:40 male wister mice were randomly divided 4 groups after five day’s adaptive feeding.They are control Group（A）, Exerice-Training group （B）,L-carnitine supplementation group（C）,and combing L-carnitine supplementation with Exerice-Training group （D）.The mice from A, and C did no exerice at all while the mice from B and D were give 6 weeks’ treadmill training（speed from 15m/min gradually increasing to 25m/min,20 to 60min/d duration with the slope at 0 degree）.The mice from D and E were perfused L-carnitine（300mg/100g.weight） between 7am and 8am each day while mice from A and C were perfused the same dose of distilled water.All the mice were killed by means of decollation after exhaustive running on the treadmill on Monday in the 7^th week.Agroupkilled without exhaustive running .Measured all SOD and MDA in mitochondria,and activitiec of respiratory complexes I , II ,III ,and IV from heart.Results:（1）After 6 week’s feeding and training , B with A to compare, D with A to compare, D with C compares, the big mouse body weight has the obvious difference, its significance level respectively is （P<0.05、P<0.01、P<0.05）。C with A to compare, non-significance difference （P> 0.05）.（2） The time of mice running to exhaustion showed significance difference between A and B、D, its level of significance respectively be （P< 0.05, P< 0.01）.Uses up between the group for the C with A non-significance difference （P> 0.05）, D with C has the significance difference （P< 0.05）.（3） compared with A,The activity of C I and CIV in mitochondria from mice heart of C revealed a significant rise（P< 0.05）, C II With CIIIActive not obvious change （P> 0.05）; B, D C I - CIV Active remarkable ascension （P< 0.01, P< 0.05, P< 0.01, P< 0.01）; With compares for C, D C I - CIV Active significance ascension （P< 0.01, P< 0.05, P< 0.05, P< 0.05）; Compares with B, D C I With CIV Active significance ascension （P< 0.05）;Compares with C, B CIIIActive significance ascension （P< 0.05）, D C I With CIV Active significance ascension （P< 0.05）.（4） Compared With A, runs to use up instantly the cardiac muscle line plastochondria SOD activeness obvious ascension the Taiwan movement for the medicine group big mouse after the strength （P< 0.05）, the MDA content significance reduces （P< 0.05）.With A to compare, B, D big mouse cardiac muscle line plastochondria SOD activeness obvious ascension （P< 0.05, P< 0.01）.Compares B with D big mouse cardiac muscle line plastochondria SOD activeness obvious ascension （P< 0.05）.Conclusion: （1） There was no effect of L-carnitine supplementation on increase of mice’s weight ,however,exercise-training could reduce the rate of mice’s weight increase.（2） There was no effect of L-carnitine supplementation on prolonging the time of mice running to exhaustion,but exercise-training could increase exhaustive time of mice running so as to improve their sports ability.（3） Supplemented the L-carnitine may obviously enhance cardioid plastochondria breath chain enzyme C I and CIVActiveness, and to enzyme C II and CIIIActiveness has certain benign influence, the promotion fatty acid tricarboxylic acid circulation as well as the oxidized phosphorylation process, for produces more ATP to provide the condition.（4）The efffect of L-carnitine on lipid peroxidation and enzymatic antioxidants,superoxide was evaluated and MDA content was decreased in heart muscle,suggesting that L-carnitine can weaken lipid peroxidation and decrease production of free rasdicals,so as to avoid or reduce the damade induced production of free rasdicals.Endurance-training also can significantly increase the cativity of superoxide dismutase,catalase,and glutathione peroxidase,and decrease MDA content,which indicated that L-carnitine supplementation had geater effect on antioxidant capacity with the cooperation of endurance-training.

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