Dissertation > Excellent graduate degree dissertation topics show

Genetic Diversity of Magnaporthe Grisea from Rice in Jiangsu and Identification of Resistance Rice Blast Gene

Author: YangHao
Tutor: ZhouYiJun;ChengZhaoBang
School: Nanjing Agricultural College
Course: Plant Pathology
Keywords: Magnaporthe grisea fingerprints code formula diversity identification of resistance rice blast gene
CLC: S435.111.41
Type: Master's thesis
Year: 2010
Downloads: 13
Quote: 0
Read: Download Dissertation


Rice blast caused by Magnaporthe grisea is the most important disease in the rice production and seriously influenced the yield and cause serious economic losses. The current selection and use of resistant varieties is still the most cost-effective method to control disease. Because of pathogenic differentiation of M.grisea resistance of rice vatieties with high resistance is easily overcome in 3-5 years. Therefore, the pathogenicity of M. grisea Variation and population genetic structure for in-depth understanding of their biological characteristics, disease resistance breeding guide on the use of resistant varieties or production of disease prevention and treatment layout have great significance.Pot2-rep-PCR technique was used to analyze the diversity of M. grisea from Jiangsu in 2007 and 2008. They were divided 14 lineages and JSL5 was dominant. The results showed that the rice blast fungus are rich in diversity in Jiangsu, but when compared with the group structure of 1999-2002, it tended to be more simple; By analyzing temporal and spatial distribution of M. grisea, we found that Population structure of M. grisea varied significantly between years and diferent rice cultivation areas of Jiangsu, but there were some M. grisea have the same fingergroup.Based on the Pot2-rep-PCR fingerprinting research into the 199 M.grisea isolates ampled from Jiangsu five rice growing areas between 2007and 2008, we analyzed the solates taking advantage of the encoding program established by Yijunzhou in 2000. The result turned out that rich polymorphism existed in the tested isolates for the two consecutive years. Significant differences were found for the tested isolates in terms of encoding programs as compared to the result obtained by Juanbai (2003).174 M.grisea isolates ware collected from rice in Jiangsu in 2007-2008, Pathpgenicity were studied with 3 sets of differentials. The results indicated that the Chinese race differentials, the Tsunematru mono-resistance-gene lines from Japan and the LTH-NILs could differentiate the 44 isolates into 10、41 and 13 pathogenic types respectively, Fl and G1 was also dominant among china-races. Comared to 67 reaction types in 2000 about Japan-race, it significantly reduced, As a whole, the blast pathogen population structure tended to be more simple. In this paper, we analyzed the genetic diversity of Magnaporthe grisea sampled from the same ecological system in Ganyu county, Jiangsu province. The results suggested that the number of code formula from a single variety was obviously less than that of the surrounding ones, moreover. M.grisea isolates under different conditions, however, shared the same dominant lineage and code formula by taking on more close origination relationship. While in the present study, significant differences were still found in the types of code formula across all the varieties.337 rice varieties were identified by Using 22 standard strains about resistance genes. Preliminary results revealed that the incidence frequency of Pik、Pia were 62.65% and 60.84%, respectively, while the frequency of Pi9、Pita2、Pikm、Pikh more then 5%. the incidence frequency of the four genes and other resistance genes was also low at the same time, they could be developed and in future research, and also could be considered to make use of in Japonica rice areas.

Related Dissertations

  1. Chinese television media discourse paradigm of the evolution of the multi-angle analysis,G220
  2. Survey and Analysis of Mycorrhizal Diversity in Zijin Mountain,S718.81
  3. Comparison of Genetic Diversity of Total Planktonic Bacteria and Active Planktonic Bacteria in Huguangyan Maar Lake in Summer,Q938
  4. Growth Pattern of Marsupenaeus Japonicus and Succesion of Plankton Community in Intensive Model,S968.22
  5. Endophytic Fungal Diversity and Their Secodary Metabolites of Eleven Dominant Plant Species in the Dry-hot Valley of Yuanjiang, Yunnan Province, China,X172
  6. Shiraia its separation of fungal genetic diversity analysis,S567.39
  7. Genetic Diversity Analyses of Sillago Sihama Using AFLP Marker,S917.4
  8. Growth Rate and Growth Model of the F5 Selected Lines for Four Shell Colors of Pinctada Martensii (Dunker) Cultured in Deepwater Area of Beibu Bay in Zhanjiang and Their Genetic Diversity Analysis Using SSR Molecular Markers,S968.31
  9. Research on Optimization of the Relay Node Location of Cooperative Diversity,TN925
  10. The Study of Winter Waterbirds’ Diversity and Habitat Selection in the West Xinghua Bay Fujian Province,Q958
  11. Preliminary Study on Bacterial Diversity in Yunnan Non-ferrous Metals Mining,TD926.4
  12. Pathogenic Differentiation and Genetic Diversity of Wheat Sharp Eyespot in Henan Province,S435.121
  13. ISSR Analysis of Genetic Diversity on 21 Lotus(Nelumbo Nucifera) Cultivars,S682.32
  14. Genetic Diversity and Ultrastructural Studies of Southern Corn Rust in China,S435.131.4
  15. SRAP and SSR molecular markers of pink flower Dendrobium genetic diversity and population genetic structure,S567.239
  16. The Research of Microsatellite Markers and the Eatablishment of Growth and Development Model in Xianan Cattle and Pinan Cattle,S823
  17. Red Soil Microbial Diversity of Peanut Continuous Cropping and Effects of Microorganisms on Growth of Continuous Cropping Peanut,S565.2
  18. Effect of Simulating Soil Environment on Soil Bacterial Diversity by PCR-RFLP Analysis,S154.3
  19. Effect of Organic Nutritiveadditives on Soil Micro-ecological Restoration and Its Mechanism.,S143
  20. Ecological Control of Tobacco Bacterial Wilt in Continous-cropping Field and the Microecological Mechanisms,S435.72

CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Crop pests and diseases and their prevention > Cereal crop pests and diseases > Rice pests and diseases > Disease > Transgression ( pass ) an infectious agent harmful > Rice blast
© 2012 www.DissertationTopic.Net  Mobile