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Study on Spores and Pollen Breaking Based on Ultrahigh-Pressure Impinging Stream Technology

Author: SunHuiLi
Tutor: TanZuo
School: Southwest Jiaotong University
Course: Biochemistry and Molecular Biology
Keywords: EHV impinging streams Pollen Breakage rate Microscopic observation Particle size distribution Determination
CLC: TQ461
Type: Master's thesis
Year: 2009
Downloads: 27
Quote: 1
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Abstract


EHV impinging stream technology is a dynamic continuous use physical methods for material crushing technology, this technology applied to broken pollen class study and solve pollen broken rate, high cost, easy to cause dust pollution and other issues, is a worthwhile research and development of pollen broken technology. Objective: To establish a new class of broken pollen method - high pressure impinging streams broken law; explore laser particle size analysis method instead of the traditional Chinese medicine microscopic quantitative method to check the feasibility of pollen breakage rate. Methods: collision type, collision in two ways, through single factor, the design L9 (34) orthogonal to breakage rate (respectively medicine microscopic quantitative method and laser particle size analysis method for the determination) as the evaluation index the pollen broken handle for high pressure impinging streams, preferably broken optimum; compared before and after the pollen in microscopic broken, particle size distribution, assay and other aspects of the changes. Results: (1) orthogonal test showed that: collision type broken way, the best solution is broken Bodhisattva A2B3C3, in broken Chinese medicine microscopic quantitation method was 87.8%, laser particle size analysis test for 88.195%; Puhuang broken the best solution for the A2B3C3, in broken Chinese medicine microscopic quantitation method was 72.3%, laser particle size analysis test for 72.046%; canola flower pink broken the best solution for the A2B3C2, to medicine microscopic quantitation method broken rate of 94.6%, a laser particle size analysis test for 93.047%: Collider law broken in the way, the best solution of the above pollen broken, broken rate was 97.6%, 96.379%; 79.5%, 79.064% : 100%, 99.661%. (2) microscopic observation showed that microscopic pollen morphological change significantly before and after broken. (3) laser particle size analysis method broken pollen particle size distribution analysis showed that the cumulative distribution of the volume average particle size, specific surface area with the pollen suspension concentration, broken times, the pressure was a regular change of the measured breakage rate and medicine microscopic quantitative method for detecting breakage rate was consistent, laser particle size analysis basically true reflection of the broken pollen particle size distribution. (4) a preliminary determination that is not broken broken pollen pollen content than content decreased. Conclusion: UHP impinging stream technology to good effect on pollen broken, and the way to the collision-type broken broken rate; laser particle size analysis used to test the broken pollen particle size distribution, the method is simple, fast, with the objective , used in place of conventional medicine quantitative method for detecting microscopic pollen broken rate is feasible; EHV impinging streams broken law, clean, safe, low cost, high efficiency, research and development is a worthwhile break pollen wall technology, and in the medical field tremendous potential applications.

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