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DNA barcode technology is a hotspot of biodiversity research in recent years, namely the use of short of universal DNA fragments species rapid and accurate identification of molecular biology methods. The Zingiberaceae is the monocots Zingiberales important pan-tropical sub-families of 52 genera in the world, more than 1,300 species, mainly distributed in tropical Asia, Africa and the Americas. Of Zingiberaceae 21 genera, 216 species, many of which are important medicinal, ornamental and spice plants, producing southwest to the southeast provinces and autonomous regions, the most abundant types of Guangdong, Guangxi, Yunnan. The Zingiberaceae system classification, origin and evolution has been controversial, morphological identification more difficult. Zingiberaceae 26 genera, 314 kinds of materials, using a variety of analytical methods screening for identification of the plants DNA barcode sequence, explore new methods of identification of medicinal plants of Zingiberaceae: 1, ginger families, 26 genera and 314 species of plants eight candidate sequences for PCR amplification, sequencing, using Clustal X software (3 coding sequence: rbcL,, matK, rpoB; 5 non-coding sequence trnH-psbA psbK-PSBI atpB - rbcL,, ITS, and ITS2 of) sequence alignment, comparison results with MEGA 4.1 variable sites, information sites and K-2P genetic distance. 2, the use of computer programming characterization of interspecific and intraspecific variation of the six parameters, namely: \\Taxon DNA Rudolf Meier designed software, the different sequences of interspecific and intraspecific variation distribution for statistical analysis, and build interspecific, intraspecific variation distribution histogram, in accordance with the evaluation of different sequences of the Merey CP definition exists interspecific and intraspecific boundaries Barcoding Gap \4, with reference to Howard A. Ross findings using Blast and Distance method to calculate the success rate of the species identification of the different sequences respectively, through computer programming, testing 8 sequence in Zingiberaceae species level identification reliability. Results: amplified using universal primers ITS, ITS2 sequence fragment length of 450 bp and 700 bp the interspecific variation .1007 and .0776 (K-2P distance), intraspecific variation of 0.0050 and 0.0036, respectively, interspecific variation and intraspecific variation between the more obvious \plant identification bar code sequence. Amplified matK to use 390F/1326R primer sequences, fragment length 890 bp interspecific variation 0.0188, intraspecific variation 0.0013 certain \matK sequence full-length as ITS2, ITS sequences complementary sequence used for the Zingiberaceae the DNA barcode identification. 5 sequence, sequence variation is small, \
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