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Objective: through the application of high-field liquid NMR spectrum instrument detected in vitro meningioma subtypes, hemangiopericytoma, concentration and brain astrocytoma and glioblastoma Ala, Glu concentration of Glu / Cho, Ala / Cho metabolite variation, and to assess these changes in metabolites in different subtypes of meningiomas and other brain tumors significance. Method :: 36 cases of intracranial tumor specimens and 6 normal brain tissue (weighing 0.5-5g), from cooperative hospital routine surgery. Specimens are quickly frozen in liquid nitrogen for 15 minutes, then placed in a depth of -80 ℃ low temperature refrigerator. Frozen samples were 0.5mmol / L perchloric acid extract preparation, the tumor pathology results from the cooperation Hospital anatomical Pathology formulated according to the 2000 WHO diagnostic criteria. Meningioma 24 cases, five cases of astrocytoma cases of hemangiopericytoma, six cases of glioblastoma, and normal white matter of the control group. The Meningioma including clinically more common epithelial type, fiber type and mixed type, gravel Body. Specimen collection method for lesion center section, to avoid bleeding, cystic degeneration. Five cases of fibrous meningioma extract the tumor edge edema in brain tissue. Vitro 1H-MRS data acquisition from the Body 9.4T high-resolution nuclear magnetic resonance spectrum analyzer (Bruker welcomes you in the new Avance 400 MHz). The resulting spectrum in the frequency domain by XWINNMR (Bruker GmBH) software preliminary treatment, apply MestRe-c 4.3 software for further analysis. Results: 1 in vitro meningioma and normal brain tissue, astrocytoma (I-II), hemangiopericytoma there Ala peak, Ala in meningiomas significantly improved, with the normal brain tissue, astrocytoma ( I-Ⅱ) concentration differences exist between Ala significant statistically significant (P <0.05); Ala peaks not seen in the cases of glioblastoma tumor cases of hemangiopericytoma MI peak can be seen. 2, Glu concentration, Glu / cho metabolite concentrations between meningioma subtype not statistically significant (P> 0.05). Ala concentration was statistically significant compared to the other three types of differences in hybrid meningeal. 3, Ala concentration, Glu concentration, Ala / Cho, Glu / cho the fibrous meningioma lesions substantial part of the tumor periphery brain tissue and normal white matter, the difference was statistically significant (P <0.05). The tumor surrounding brain tissue and normal brain tissue between Ala Ala / Cho, Glu, Glu / cho difference was not statistically significant (P> 0.05). 4, Ala concentration, Ala / Cho astrocytoma (I-II) with normal white matter, fibrous meningioma difference was statistically significant (P <0.05). Glu concentration, Glu / cho difference in astrocytoma (I-Ⅱ), glioblastoma, fibrous meningioma normal brain tissue was statistically significant (P <0.05). Conclusion: We believe that the 9.4T vitro high-field magnetic resonance spectrum quantitative detection of brain tumor metabolites able to obtain high-resolution magnetic resonance spectrum in Fig. The experiment suggests a concentration of high-Ala, Glu concentration, Ala / Cho, Glu / cho is characteristic of meningioma concentration Ala, Ala / Cho mixed meningioma compared to the other three types of low may characterized, and its value to be further studies. Concentrations of Ala, Glu concentration, Ala / Cho, of Glu / cho was no significant difference between benign fibrous meningioma surrounding brain tissue and normal white matter, which prompted meningioma surrounding brain tissue edema the mechanism no clear erosion may. Glu concentration, Glu / cho astrocytomas (I-Ⅱ), glioblastoma, normal white matter, fibrous meningioma difference was statistically significant. With the improvement of the degree of malignancy of the tumor, suggesting that the increase in the tumor is not homogeneous, metabolites change increases.
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