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Objective: To form deprivation myopia in guinea pig model of choroidal injection of exogenous TIMP-2 gene was observed early after myopic sclera expression of MMP-2, TIMP-2 study exogenous gene MMP posterior sclera -2 expression. Methods: monocular cover method tricolor guinea pigs 3 weeks of form deprivation, 2w adopted after retinoscopy and axial length measurements confirmed FDM model is successfully established. From the model to build a successful 60 guinea pigs were randomly selected and randomly divided into four groups, namely TIMP-2 group (choroidal injection of exogenous TIMP-2 gene), empty plasmid group (choroidal empty plasmid injection), physical saline group (choroidal injection of saline), control group 15. TIMP-2 group, empty plasmid, saline injection operation on the right to continue to cover the completion of which TIMP-2 group left for their own group; control group continued to cover the eye without any treatment. After injection, respectively, 2d, 7d, 14d posterior sclera specimens collected using RT-PCR and Western Blot assay MMP-2 mRNA and protein expression, statistical analysis between the groups and the differences in the expression of different times. Results: (1) after injection of TIMP-2 group, MMP-2 mRNA and protein expression appeared first decreased and then increased variation after injection 2d and 7d MMP-2 mRNA and protein expression are significantly different (P lt; 0.05) , 7d and 14d expression was no significant difference (P gt; 0.05). After injection 2d, 7d, 14d, TIMP-2 group, self-control group, the comparison between the control group, MMP-2 mRNA and protein expression changes were statistically significant (all P lt; 0.05); empty plasmid group, physiological saline group, the control group, the difference was not statistically significant (all P lt; 0.05). (2) after injection of TIMP-2 group of TIMP-2 mRNA expression increased at first and then decreased changes. 2d and 7d after injection expression changes were statistically significant (P lt; 0.05), 7d and 14d were no significant differences (P gt; 0.05). After injection 2d, 7d, 14d, TIMP-2 group, self-control group, the comparison between the control group, TIMP-2 mRNA expression changes were statistically significant (all P lt; 0.05); empty plasmid, saline, comparison between the control group, no significant changes in expression (both P gt; 0.05). CONCLUSION: Exogenous TIMP-2 gene transfection can inhibit form deprivation myopia posterior sclera MMP-2 mRNA and protein expression, this change has already started to occur early, with the extension of time after transfection appears first and then increased changes.
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