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Objective: fluorescence in situ hybridization (FISH) to detect cervical intraepithelial neoplasia (CIN) and cervical cancer in the TERC gene amplification , and its significance in the screening of cervical lesions . Methods: the outpatients cervical disease screening cervical exfoliated cell samples 120 cases , including 20 cases of liquid-based cytology normal , pathological examination of 25 cases of CIN Ⅰ, Ⅱ, Ⅲ and cervical cancer . Using two - color interphase FISH to detect cervical exfoliated cells TERC gene amplification threshold test results to establish normal specimens . Results: ① threshold of 6.27% . CIN Ⅰ, Ⅱ, Ⅲ and cervical cancer in each case the percentage of abnormal cells is greater than the threshold value ( P lt; 0.05 ) , the percentage of abnormal cells with pathological grade increased severity increased ( P lt; 0.01 ) ; 2 2:3 2:4,2:5 and 4:4 type in CIN Ⅰ, Ⅱ, Ⅲ and cervical percentage significantly different (all P lt; 0.01 ) , including cervical cancer and CIN Ⅰ comparison , the proportion of 2:3 type decreased significantly ( P lt; 0.001 ) , more than one type of 2:4,2:5 and 4:4 proportion was significantly higher ( P lt; 0.001 ) . ③ different cytology classification , the percentage of abnormal cells with increased severity increased ( P lt; 0.01 ) , 2:3,2:5 and 4:4 type there are significant differences in the percentage of ASCUS, LSIL and HSIL (all P lt; 0.01) which HSIL and ASCUS compare the proportion of 2:3 type decreased (P lt; 0.01), 2:5 and 4:4 type proportion was significantly higher (P lt; 0.01) ; ④ low - grade and high - grade CIN , cytology LSIL and HSIL detection rates were 10 cases (40% , 10 / 25 ) and 31 patients (62 % , 31 /50 ) , FISH technology detection rate of 100 % , significantly higher than cytology ( P lt; 0.05 ) . Conclusion : CIN Ⅰ, Ⅱ, Ⅲ and cervical cancer have TERC gene amplification , copy number with pathology and cytology grading severity increases . FISH technique detects cervical exfoliated cells TERC gene amplification has a certain value of the screening of cervical lesions and lesions progress forecast .
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