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The purpose of the use of proteomics technology and rats with acute spinal cord injury (acute spinal cord injury, ASCI) model ligustrazine treated with saline treatment after spinal cord proteome expression profile differences, the differences in protein identification tetramethylpyrazine role. And preliminary differential protein function analysis, from the level of protein molecules reveal tetramethylpyrazine mechanism for the treatment of acute spinal cord injury in rats. Normal adult SD rats 12, improved ALLEN's weights to combat moderately acute spinal cord injury model 12. Randomly divided into two groups: (1) normal saline (NS group, N group) (n = 6); (2) ligustrazine treated group (TMP group, T group) (n = 6). Two groups of rats postoperative animal survival time 3 days and 7 days group then each divided into two subgroups, each subgroup of three rats. TMP group 30 minutes after injury, rats began to be the Chuanxiong Qin injection by intraperitoneal injection of 200mg/kg, given once a day, 3 days rats medication 3 days, 7 days rats medication for five days. NS rats given saline by intraperitoneal injection, the frequency of administration, and the same as the experimental group. Rats were sacrificed at the corresponding point in time after modeling and cut the spinal cord specimens, taken injury segment as the center of about 6mm each rat spinal cord tissue. Lysis fluid extraction spinal protein, after measuring the concentration of protein samples. 1000ug sample volume, two-dimensional gel electrophoresis, protein gel, using image analysis software using PDQuest-8.0.1 Coomassie blue staining after gel image analysis, locate and cut the differentially expressed protein spots. In situ gel with trypsin digestion, small peptide fragments obtained using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) mass spectrometry analysis. For peptide mass fingerprinting MascotWizard software queries the SWISS-PROT and NCBInr database retrieval and identification of differentially expressed proteins. To retrieve the identification of differentially expressed proteins based on the literature review, preliminary functional analysis. Results obtained by two-dimensional gel electrophoresis of the solid phase PH gradient high-resolution two-dimensional gel electrophoresis pattern, image analysis, and found that there were obvious differences between the two sets of maps, matrix-assisted laser desorption ionization time-of-flight mass spectrometry successfully identified a differences in protein. 3-day group were identified 15 differentially expressed proteins, expression raised the serum albumin precursor, the low molecular weight neurofilament protein, vimentin, nerve fibrillary acidic protein, α-1A microtubule tubulin protein, β-2A β-2C microtubule protein, leucine aminopeptidase, the dihydropyrimidine enzyme associated protein 2 downregulated α-interconnected protein 60kDa heat shock protein 71kDa heat shock protein homologous protein, 70kDa heat shock protein-related protein 2 β-1 heat shock protein, phospholipids binding protein 1; 7 days group identified a total of 19 differentially expressed proteins expression raised a serum albumin precursor, α-1A tubulin, β-2A tubulin, low molecular weight neurofilament medium molecular weight neurofilament protein, aldehyde dehydrogenase, guanine nucleotide binding protein beta subunit, β-actin, vimentin, neural fibrillary acidic protein, downregulated H2 heterosexual nucleoprotein Parkinson's disease protein homologues, vesicle-associated membrane protein B, ubiquitin conjugating enzyme E2N, pyruvate dehydrogenase E1β subunit, isocitrate dehydrogenase α subunit of brain-type creatine kinase, α-interconnected protein, 60kDa heat shock protein. Combination of literature review, differential protein involved in nerve cell proliferation, apoptosis, stress response process. Conclusions Acute Spinal Cord Injury ligustrazine treated group and the saline treatment group proteomic expression profile differences, the differential protein is mainly related to the process of nerve cell proliferation, apoptosis, stress response. Provides a theoretical basis for further clarify the mechanisms of spinal cord injury repair mechanisms and tetramethylpyrazine treatment of acute spinal cord injury in rats.
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