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The main active ingredients of the Rubiaceae Gardenia iridoid class geniposide ( geniposide ) , protective and choleretic , anti-inflammatory , anti-tumor, anti-mutagenic , anti-oxidation , anti- thrombotic , anti-hypertensive , protection nerve cells to repair damaged tissue , hypoglycemic and pharmacological activity ; hypoglycemic effect in recent years, in particular, are of great concern . Studies have shown that the geniposide most of the biologically active aglycone genipin (genipin) . This topic first investigated geniposide in gardenia fruit distribution , HPLC analysis showed that geniposide mainly exists in the Gardenia nuts , peel content . Second , for the first time using flash extraction method to improve the extraction process of the bitter almond enzyme , and optimization of the process , come to The optimum extraction conditions : 50.0 g skim bitter almond powder 0.0625mol / L pre-cooling ammonia (500mL 450mL), the flash and extracted twice ( 120s 90s ) , gauze filtered, and the filtrate was collected , the acetic acid pH4.0 , filtered off and the hybrid protein , four times the amount of ethanol precipitation bitter almond enzyme and filtered, washed and dried , chilled ie ; with DNS method for the determination of the specific activity of the enzyme bitter almond . Again, the four-factor and five levels of the enzymatic hydrolysis of bitter almond extract geniposide quadratic general rotary trial design to optimize the hydrolysis process indicators to hydrolysis was investigated buffer pH , reaction temperature, reaction time and join the four main factors in the amount of enzyme to process ; determine the appropriate process conditions : buffer pH5.25 , the reaction temperature is 50.5 ℃, the reaction time was 90min , the addition of the enzyme 10 ; through the physical and chemical properties , purity test identified by NMR to determine the hydrolyzate is genipin . Finally, alloxan diabetic mouse model study geniposide and genipin hypoglycemic effect ; results show that , in a certain dose range , geniposide , genipin average can improve the survival rate of mice model ; reduce blood glucose levels of diabetic mice , hypoglycemic effect .
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