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Comparison of waaF Gene of Guillain-Barré Syndrome-associated Campylobacter Jejuni Strains

Author: ChenJuan
Tutor: LiZhenZhong
School: Hebei Medical University
Course: Neurology
Keywords: Guillain - Barre syndrome Campylobacter jejuni waaF gene Sequence analysis Secondary structure
CLC: R745.43
Type: Master's thesis
Year: 2010
Downloads: 6
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Abstract


Objective:? Determination of three Guillain - Barre syndrome (GBS) Campylobacter bacteria (Campylobact-er jejuni, C.jejuni) by the waaF gene sequence and the corresponding gene sequences and the GeneBank caused diarrhea C.jejuni NCTC11168 Compare to understand the cause of GBS C. jejuni strain waaF gene sequence of the nucleic acid and amino acid sequence characteristics, to explore C.jejuni caused by GBS mechanism, and analyze the genetic evolutionary relationships. Methods: Hebei Medical University Department of Neurology, patients with GBS isolated from feces and confirmed by animal models for the three C.jejuni caused by GBS strains were cultured to extract DNA sequencing of its genome. Application NCBI Blast, SequenceAlignment the DNASTAR EditSeq, MegAlign bioinformatics software will be three C.jejuni strain genome sequence to the sequence in Genbank NCTC11168 as controls for comparison, identify gene mutations waaF base, and deduced amino acid sequences were compared to identify mutations in amino acids; application DNASTAR Protean software of its encoded protein structure were analyzed to understand the changes in the secondary structure, hydrophobicity, active sites, the research waaF gene GBS patient relationship; MegAlign software, the three C.jejuni waaF gene sequences and deduced amino acid sequence with the sequence alignment to calculate the genetic distances between sequences, to explore its geographical features. Results: 3 caused by GBS strains of C. jejuni waaF gene by 960 base. Compared with NCTC11168 waaF of gene sequences, the strains zhanxing total of 18 base mutation, resulting in five amino acid changes and a total of 14 base mutation; qiaoyuntao strain, resulting in five amino acid changes and a total of 16 base mutation; Lulei strain, resulting in 6 amino acid change. 5 3 caused by GBS strains of the same base mutation sites: 1081162C → T the 1081273T → C the 1081630A → T, of 1,081,384 T → C, 1081462zhanxingT → A Lulei qiaoyuntaoT → G, the same amino acid change: 185 D → E, the point lead to 3 caused by GBS C. jejuni strains alpha helix changed. In addition to the five bases the zhanxing strains and lulei of strains, the same mutation in the nucleotide 3: 1080974A → G the 1081226T → C the 1081129A → G, the same amino acid change: 107 Y → H, the locus result in this 2 caused by GBS strains of C. jejuni random coil structure has changed; the qiaoyuntao strains with lulei of strains as well as the same mutated bases 2: 1081773C → T, 1081808C → T, led to a common amino acid change: 289 T → . These nucleotide and amino acid mutations may Campylobacter bacteria caused by GBS ability to change the relevant. Genetic distance, strains zhanxing and the qiaoyuntao strains to 2.5%, the zhanxing strains with lulei of strains of 2.0%, 1.7% qiaoyuntao strains with lulei of strain; deduced amino acid sequence of the genetic distance: zhanxing the strain qiaoyuntao strains to 1.9%, zhanxing strains with the strains lulei 2.6%, the qiaoyuntao strains with lulei of strains of 2.2%. Conclusion: Compared with diarrhea caused by strains, of GBS related C.jejuni strains waaF gene sequences there are 1,081,462 zhanxing T → A, Lulei the same sites qiaoyuntaoT → G nucleotide mutation and the corresponding 185 D → E amino acid changes, and the The secondary structure of these nucleotide and amino acid mutations may be curved bacteria caused by GBS ability to change with the jejunum related. the zhanxing, qiaoyuntao, lulei 3 strains caused by GBS Campylobacter bacteria waaF the genetic distance is small, reflecting the Hebei region caused by GBS jejunum Campylobacter clustering phenomenon exists in evolution, certain regional characteristics.

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