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Ning treatment of esophageal reflux esophagitis experimental study

Author: TangSenHai
Tutor: GanAiPing
School: Hubei University of Chinese Medicine
Course: Chinese medical science
Keywords: Reflux esophagitis Esophageal Ning Proliferating cell nuclear antigen Experimental study
CLC: R571
Type: Master's thesis
Year: 2010
Downloads: 49
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Abstract

Objective: To observe the anti-ning esophageal reflux esophagitis rat model of esophageal histopathological changes and PCNA (Proliferating cell nuclear antigen, PCNA) effects, which studies Ning esophageal reflux esophagitis on the role and mechanism of action , explore its modulating reflux esophagitis scientific basis. Methods: The first step: modeling and treatment of clean grade 8 week-old male SD rats were 40, weight (200 ± 20) g, were randomly divided into four groups, each 10. Take a group as a blank control group (A), normal feeding 7 days. 7 days after the remaining three groups according to the literature and improved modeling, OK \Control group with normal saline 14 days; remaining three groups were known as the model control group (B), esophagus Ning Tang treatment group (C group), omeprazole treatment group (D group), group and continue to feed 7 days after administration. Group B I saline 2m1 gavage once a day; C group I omeprazole suspension 2ml orally once a day (every m1 containing crude drug 0.36mg); D group Ning Tang solution 2m1 esophageal gavage, a once a day (every m1 containing crude drug 1.2g), were administered for 14 days. Rats were observed before and after treatment general condition, body weight and food intake. Step Two: Indicators detect visually rat esophageal manifestations: treatment rats were sacrificed after 14 days from the gastroesophageal junction on 0.5mc interception at 2cm esophagus to the throat for the naked eye. Light microscope rat esophageal tissue pathological changes: for the naked eye after fixed with 10% formalin, sliced, doing light microscope. Ning on rats observed esophageal esophagus before and after treatment of the organizational structure. PCNA immunohistochemical examination: Immunohistochemical staining was observed esophageal Ning PCNA rats before and after treatment of the esophagus. Results: 1. General: blank control group activities sensitive, coat shiny, normal diet. After modeling after the rats have different levels of activity due to quick, slow movement, hair loose, shiny weakened, dull, eating less, bloating, big poo thin, weight stagnating or even reduced. Esophagus Ning group and omeprazole group given treatment, the symptoms and signs gradually improved, but the speed of recovery is faster than western medicine group, group, and ultimately esophageal Ning group, omeprazole rats were returned to normal. (2) the detection results of the indicators: 2.1 Macroscopic observation of esophageal mucosa Points: Points of view from the naked eye, the model control group was significantly higher than the control group, there was a significant difference (P lt; 0.01). Two weeks after the treatment of esophageal Ning Tang omeprazole group and the control group compared with the model group was significantly lower, there were significant differences (P lt; 0.01), compared with the control group, there was no difference (p gt; 0.05 ). Esophagus Ning Tang group no difference compared with omeprazole (gt; P0.05). Tip esophagus rather to improve anti-reflux esophagitis rat model of esophageal mucosa situation. 2.2 esophageal mucosa light microscope Points: Points from the pathological point of view, the model control group was significantly higher than the control group, there was a significant difference (P lt; 0.01). Ning Tang esophagus omeprazole group and model group and the control group, was significantly lower after two weeks of treatment, there were significant differences (P lt; 0.01). Esophagus Ning Tang group and omeprazole group compared with the control group, there were no differences in the two weeks after treatment (p gt; 0.05). Esophagus Ning Tang group and comparison group showed no difference (P gt; 0.05). Tip esophagus may be improving Ning rats esophageal mucosa structure the RE onset. 2.3 esophageal mucosa PCNA immunohistochemical staining of gray values: From PCNA immunohistochemical staining gray value point of view, the model control group was significantly higher than the control group, there was a significant difference (P lt; 0.01), esophagus Ning Tang group and omeprazole in the treatment of two weeks after its PCNA immunohistochemical staining gray value compared with the model group was significantly lower, there is a significant difference (p lt; 0.01); compared with the control group, both still high compared with omeprazole group is still a significant difference (p lt; 0.05), but the esophagus Ning Tang group with no significant difference (p gt; 0.05). Esophagus Ning Tang group and omeprazole group had significant difference compared to (p gt; 0.05). Tip esophagus rather may be inhibited PCNA expression in rat esophageal mucosa increased and the RE onset. Conclusion: Treatment of reflux esophageal Ning esophagitis have good effect, can improve the rat model of esophageal mucosa pathological conditions, inhibition of PCNA expression in esophageal mucosa increased, indicating that PCNA in reflux esophagitis rats play an important role.

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