Dissertation > Excellent graduate degree dissertation topics show

The Protective Effect of Trans-resveratrol on Rats Hippocampus Injury by Aβ25-35

Author: WangShunWang
Tutor: XuPing
School: Zunyi Medical College,
Course: Neurology
Keywords: Trans- resveratrol (TR) Rats caspase-8 caspase-9 Inducible nitric oxide synthase
CLC: R285.5
Type: Master's thesis
Year: 2010
Downloads: 26
Quote: 0
Read: Download Dissertation

Abstract


Objective To observe the protective effect of rat hippocampus trans-resveratrol (TR) Aβ25-35 injury . Method rat Morris water maze training , were randomly divided into five groups: (1) sham group ; (2) the model group ; ( 3 ) - ( 5) were TR high , medium , low dose group . Injection of Aβ25-35, the molding with TR intervention designed to observe the TR Aβ25-35 -induced neurotoxicity in the rat hippocampus . Rats in each group using the water maze spatial discrimination learning and memory ; 5 hippocampal apoptosis TUNEL assay ; using real-time RT-PCR assay hippocampal and cortical caspase -8 9 , inducible nitric oxide synthase (iNOS) mRNA expression . Results hippocampus of rats injected with Aβ25-35 molding escape latency in the water maze and search to find distance significantly longer , hippocampal caspase -8 , 9 mRNA , iNOS expression increased hippocampal tissue increased apoptotic cells . While the the TR intervention group decreased expression of apoptotic cells also reduced . ( 1 ) group to group of caspase-9mRNA the expression of the PCR method detection of caspase-8mRNA the expression levels were 0.27 ± 0.09,1.34 ± 0.87,0.24 ± 0.09,0.29 ± 0.06,0.64 ± 0.15; ( 5 ) group were 0.34 ± 0.07,0.98 ± 0.17,0.32 ± 0.13,0.44 ± 0.18,0.91 ± 0.25; groups iNOS mRNA expression were 0.23 ± 0.90,1.23 ± 0.91,0.34 ± 0.18 , 0.48 ± O.12 0.87 ± 0.49. All the intervention group compared with the control group (P lt; 0.01) were different . TUNEL assay hippocampal specimens hippocampal cells under a fluorescence microscope apoptotic index were 6 ± 3,24 ± 3,7 ± 2,11 ± 4,20 ± 3 (%), the intervention group and the control group compared to (P lt; 0.01) differences . TUNEL assay ( 1 ) to (5 ) group, the apoptosis index ( after 11 days) were : 3.23 ± 0.52,21.32 ± 5.02,5.72 ± 1.56,7.21 ± 1.22,10.93 ± 3.25 ( %) ; ( surgery after 11 days) were 3.21 ± 0.29,16.31 ± 4.27,5.13 ± 2,7.12 ± 1.78,9.84 ± 2.66 (%), no differences ( P gt; 0.05 ) compared with the apoptotic index . Conclusion TR reduce Aβ25-35 - induced memory impairment in rats , and its mechanism may be partly reduced hippocampal caspase -8 , 9 , iNOS mRNA expression reducing hippocampal apoptosis related .

Related Dissertations

  1. Effects of CrPyr on Protein Metabolism in Rats,S865.12
  2. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  3. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  4. Initial Research on the Expression and the Significance of Cx-43、 Caspase-3 and MMP-2 in Endothelial Cells in Atherosclerosis in Human Coronary Artery,R541.4
  5. Expression and Clinical Significance of PTEN and Caspase-7 in Gastric Cancer and Precancerous Lesion,R735.2
  6. Expression and Correlation of Caspase-3 and Cx43 in Myocardial Infarction with Different Narrow Degree Coronary Artery,R542.22
  7. Expression of Caspase-4 and Caspase-3 Gene Related Apoptosis in Pathological Changes of Sternocleidomastoid of Congenital Muscular Torticollis and Four Years Clinical Followed-up for Opereated Patients,R726.8
  8. Mushroom C 91-3 fermentation broth preliminary identification of protein purification and anti-tumor mechanism,R730.5
  9. Roles of Human Heart Developmental Candidate Genes ZNF382 and CXXC5,Q987
  10. Study of Expression and Significance of Tumor Suppressor Gene Maspin and Caspase-3 and MVD in Epithelial Ovarian Cancer,R737.31
  11. inhibitory Effect of Immunosuppressive Agent FTY720 on Hepatocellular Carcinoma Hepal-6 in Vitro Experimental Study,R735.7
  12. Polymorphism of Caspase-3 -928A/G Associated with the Risk and Aggressiveness of Colorectal Cancer in Chinese Population,R735.3
  13. The Neuroprotection of Recombinant Human Granulocyte Colony-Stimulating Factor Given Intranasally to Central Nervous System on Brain Injury Secondary to Subarachnoid Hemorrare,R743.3
  14. The Effects of Atorvastatin on Expression of Osteopontin in Kidney of the Diabetic Rats Its Molecular Mechanism,R587.2
  15. The Relation between Expression of IL-8、 ICAM-1 in Tissue Surrounding the Hematoma and Brain Edema in Experimental Intracerebral Hemorrhage and the Intervention Role of Injection of Ginkgo Biloba Leave Extract,R743.34
  16. The Effects of Acid-sensing Na +/h + Exchanger Inhibitor Cariporide on LPS-induced Acute Lung Injury in Rats,R965
  17. The Death Pattern of Bovine Kidney(mdbk) Cells Induced by Foot-and-mouth Disease Virus (fmdv)Lproand Molecular Mechanism,S852.65
  18. Different to Stroke Hemiplegia Supersession Acupuncture Rats Spasticity PKA Content of Impact Study,R246
  19. Chronic visceral hyperalgesia and acupuncture to ease the neurobiological mechanisms of visceral pain,R245
  20. Of tea polysaccharide of glucose and lipid metabolism , metabolic syndrome rats and Mechanism,R285.5
  21. Gossypol on human fibrosarcoma HT1080 cells apoptosis and its related mechanism,R285

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
© 2012 www.DissertationTopic.Net  Mobile